Antimicrobial resistance of Campylobacter isolates from small scale and backyard chicken in Kenya

Nguyen, Tuan Ngoc; Hotzel, Helmut; Njeru, John; Mwituria, Joyce; El-Adawy, Hosny; Tomaso, Herbert; Neubauer, Heinrich; Hafez, H. M.

doi:10.1186/s13099-016-0121-5

Cited by 53 publications

(66 citation statements)

References 40 publications

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“…In the Campylobacteriaceae, C. coli, C. fetus, and C. jejuni were detected in cow rumen fluid and feces (Table 1). Similar findings were reported in Tanzania, Kenya and Ghana by (Kashoma et al, 2015;Nguyen et al, 2016;Karikari et al, 2017) in beef cattle feces, faeces and cloacal swabs of chickens and faeces and carcasses of healthy livestock animals, respectively. Pathogenic bacteria Staphylococcus aureus and Staphylococcus sciuri were detected only at the smallholder farms in Tanzania (Table 1).…”

Section: Blaaccsupporting

confidence: 86%

Metagenomic analysis of enteric bacterial pathogens affecting the performance of dairy cows in smallholder productions systems

Habimana¹,

Bett²,

Amimo³

et al. 2018

Afr. J. Microbiol. Res.

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There is little information about the diversity of bacterial pathogens present in the rumen and feces of healthy cow and the subsequent effects on the performance of the host animal. The objectives of the present study were to genetically characterize the enteric bacterial pathogens found in the rumen fluid and cow feces and to identify the resistant genes responsible for antimicrobial resistance in the detected pathogens. The cow feces and rumen fluid samples (6 rumen fluid and 42 feces) were collected from lactating dairy cows. Using next generation sequencing, the enteric bacterial pathogens detected were screened for antimicrobial resistance genes using ResFinder-2.1 database in the center of Abricate. The characterized enteric bacterial pathogens include Escherichia coli, Salmonella enterica, Streptococcus agalactiae, Streptococcus pyogenes, Campylobacter coli, and Campylobacter fetus among others. Those enteric bacterial pathogens were also drug resistant bacteria except Campylobacter coli. The Campylobacter fetus fetus was identified as the only multidrug resistant bacterial pathogen detected in the cow feces. However, the abundant resistant genes detected confer resistance to tetracycline (17 genes from 209 contigs), beta-lactam (21 genes from 67 contigs), streptomycin (6 genes from 153 contigs), and sulfamethoxazole (2 genes from 72 contigs). This is the first study to identify the diversity of enteric bacterial pathogens from the station based and smallholder dairy cows in Kenya and Tanzania, respectively.

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Section: Blaaccsupporting

confidence: 86%

Metagenomic analysis of enteric bacterial pathogens affecting the performance of dairy cows in smallholder productions systems

Habimana¹,

Bett²,

Amimo³

et al. 2018

Afr. J. Microbiol. Res.

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“…In a study carried out by Padungtod et al (49), fluorogenic PCR was used to detect the molecular mechanisms of the resistance and the mutation was found to be present in the gyrA of the strain with ciprofloxacin MIC of 2 mg/L, although the strain was considered as susceptible since the breakpoint for the resistance was regarded as ≥4 mg/L according to the CLSI. In another study in which breakpoint for resistance was accepted as 0.5 mg/L in accordance with EUCAST, strains with ciprofloxacin MIC of 1 and 2 mg/L were found to have the mutation (40). On the other hand, Hormeno et al (41) did not detect any mutation by MAMA-PCR in a strain with ciprofloxacin MIC of 2 mg/L.…”

Section: Discussionmentioning

confidence: 95%

Molecular and phenotypical investigation of ciprofloxacin resistance among Campylobacter jejuni strains of human origin: high prevalence of resistance in Turkey

İlktaç

Öngen

Humphrey

et al. 2019

APMIS

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Campylobacteriosis is one of the most frequently reported zoonoses worldwide. The well‐documented increase in the ciprofloxacin resistance has increased the importance of rapid detection of the resistance. The incidence of ciprofloxacin resistance was investigated using real‐time PCR. Identification of one hundred and fifty‐eight strains was performed by PCR. Minimum inhibitory concentration (MIC) of ciprofloxacin was determined by Epsilometer test. Following the confirmation of the efficiencies of singleplex real‐time PCR methods using two different probes, a cytosine to thymine point mutation at codon 86 was detected by allelic discrimination. Of the 158 strains, 114 (72.2%) were determined to be resistant to ciprofloxacin. The MIC50 and the MIC90 of ciprofloxacin were found to be 8 and ≥32 mg/L, respectively. By real‐time PCR, the presence of the mutation was confirmed in all, but one, resistant strains and the absence of the mutation was demonstrated in all, but one, susceptible strains. The rate of resistance is high among C. jejuni strains and ciprofloxacin should not be used in the treatment of such infections in Turkey. A cytosine to thymine mutation is the most frequently detected mechanism for the resistance. Real‐time PCR can be used for the quick screening of the resistance.

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“…These isolates showed a high rate of resistance to nalidixic acid, tetracycline and ciprofloxacin of 77.4, 71.0 and 71.0%, respectively. Low resistance (25.8%) was detected for gentamicin and chloramphenicol and 61.3% of C. jejuni isolates exhibited multidrug resistance and 54.5% of the C. jejuni isolates possessed the tet(O) gene whereas all of C. coli had the tet(A) gene [74].…”

Section: Campylobacter Speciesmentioning

confidence: 99%

Antibiotic Use in Poultry Production and Its Effects on Bacterial Resistance

Agyare¹,

Boamah²,

Zumbi³

et al. 2019

Antimicrobial Resistance - A Global Threat

192

167

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A surge in the development and spread of antibiotic resistance has become a major cause for concern. Over the past few decades, no major new types of antibiotics have been produced and almost all known antibiotics are increasingly losing their activity against pathogenic microorganisms. The levels of multi-drug resistant bacteria have also increased. It is known that worldwide, more than 60% of all antibiotics that are produced find their use in animal production for both therapeutic and non-therapeutic purposes. The use of antimicrobial agents in animal husbandry has been linked to the development and spread of resistant bacteria. Poultry products are among the highest consumed products worldwide but a lot of essential antibiotics are employed during poultry production in several countries; threatening the safety of such products (through antimicrobial residues) and the increased possibility of development and spread of microbial resistance in poultry settings. This chapter documents some of the studies on antibiotic usage in poultry farming; with specific focus on some selected bacterial species, their economic importance to poultry farming and reports of resistances of isolated species from poultry settings (farms and poultry products) to essential antibiotics.

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Antimicrobial resistance of Campylobacter isolates from small scale and backyard chicken in Kenya

Cited by 53 publications

References 40 publications

Metagenomic analysis of enteric bacterial pathogens affecting the performance of dairy cows in smallholder productions systems

Metagenomic analysis of enteric bacterial pathogens affecting the performance of dairy cows in smallholder productions systems

Molecular and phenotypical investigation of ciprofloxacin resistance among Campylobacter jejuni strains of human origin: high prevalence of resistance in Turkey

Antibiotic Use in Poultry Production and Its Effects on Bacterial Resistance

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