Antimicrobial Resistance of Staphylococcus aureus Strains Acquired by Pig Farmers from Pigs

Oppliger, Anne; Moreillon, Philippe; Charrière, Nicole; Giddey, Marlyse; Morisset, Delphine; Sakwińska, Olga

doi:10.1128/aem.01902-12

Cited by 67 publications

(74 citation statements)

References 41 publications

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“…17,20,26 The 3 MLST types (ST9, ST398, ST5) detected in the present study correspond closely with those reported in studies of commercial pigs in Europe, 1,21 which is significant for 2 reasons. First, this similarity, despite different study designs and considerable temporospatial separation of the study populations, suggests that these lineages may be widely distributed among commercial swine populations.…”

Section: Discussionsupporting

confidence: 89%

The effect of anatomic site and age on detection of Staphylococcus aureus in pigs

et al. 2014

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Abstract. Despite active research into methicillin-resistant Staphylococcus aureus (MRSA) in pigs since 2004, the ecology of the susceptible ancestral organism has been neglected. A longitudinal study of pigs in 2 intensive production systems was conducted to investigate the effects of age and anatomical site on detection of S. aureus. Sampling was replicated in 2 cohorts per farm, with swabs collected from the nares, tonsils, skin (axilla), and rectum in lactating sows, suckling, weaned, and market-age pigs, plus the vagina of sows. No MRSA were isolated, but S. aureus was detected in a least 1 site in 175 (91.1%) out of 192 pigs. Pig-level prevalence did not differ among the age groups, but the proportion of positive samples (all sites) was higher in market-age pigs (75.2%) and nursery-age pigs (63.2%) than in sows (40.7%) and suckling piglets (38%). Prevalence did not differ among nasal (67.9%), skin (62.3%), and tonsil (61.7%) swabs, but was lower in rectal (42%) and vaginal swabs (39.6%). Multiple multilocus sequence typing (MLST) and spa types were found in both production systems, but all isolates were of ST398, ST9, or ST5. These MLST lineages have been variably predominant among reports of MRSA in pigs on 3 continents, and the presence of methicillin-sensitive variants in several countries raises the likelihood that MRSA in pigs has likely resulted from independent acquisition of the mecA gene by multiple S. aureus lineages that have been adapted to swine over the long term, rather than recent introduction of novel clones into swine populations.

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Section: Discussionsupporting

confidence: 89%

The effect of anatomic site and age on detection of Staphylococcus aureus in pigs

et al. 2014

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“…The carriage of S. aureus has been evaluated revealing that S. aureus colonization is common in free-living artiodactyls (Table 1). However, the carriage rates detected in the present study are lower than those reported in different domestic animals such as pigs (36%), small ruminants (from 29 to 64%), donkeys (50%), and rabbits (56%) (26)(27)(28)(29)(30)(31). In contrast to the results obtained for MRSA (17), the detection rate of MSSA in the Eurasian griffon vulture was lower than in the Iberian ibex, red deer, and wild boar, although the differences were not significant (P ϭ 0.057).…”

Section: Discussioncontrasting

confidence: 67%

Carriage of Staphylococcus aureus by Free-Living Wild Animals in Spain

Porrero

Mentaberre

Sánchez

et al. 2014

Appl Environ Microbiol

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eThe presence of methicillin-susceptible Staphylococcus aureus (MSSA) was analyzed in different free-living wild animals to assess the genetic diversity and predominant genotypes on each animal species. Samples were taken from the skin and/or nares, and isolates were characterized by spa typing, multilocus sequence typing (MLST) and antimicrobial susceptibility testing. The proportion of MSSA carriers were 5.00, 22.93, 19.78, and 17.67% in Eurasian griffon vulture, Iberian ibex, red deer, and wild boar, respectively (P ‫؍‬ 0.057). A higher proportion of isolates (P ‫؍‬ 0.000) were recovered from nasal samples (78.51%) than skin samples (21.49%), but the 9.26% of red deer and 18.25% of wild boar would have been undetected if only nasal samples had been tested. Sixty-three different spa types were identified, including 25 new spa types. The most common were t528 (43.59%) in Iberian ibex, t548 and t11212 (15.79% and 14.04%) in red deer, and t3750 (36.11%) in wild boar. By MLST, 27 STs were detected, of which 12 had not been described previously. The most frequent were ST581 for Iberian ibex (48.72%), ST425 for red deer (29.82%), and ST2328 for wild boar (42.36%). Isolates from Eurasian griffon vulture belong to ST133. Host specificity has been observed for the most frequent spa types and STs (P ‫؍‬ 0.000). The highest resistance percentage was found against benzylpenicillin (average, 22.2%), although most of the S. aureus isolates were susceptible to all antimicrobial tested. Basically, MSSA isolates were different from those MRSA isolates previously detected in the same animal species.

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“…Approximately 25%-30 % of healthy humans carry S. aureus in their nasal cavity, and nasal carriage is a recognized risk factor for S. aureus infection [1,2]. Other hosts of S. aureus include pigs, where a similar prevalence of carriage as in humans has been reported in Switzerland [3]. S. aureus can also be found in dogs and cats; however, in dogs a related coagulase-positive staphylococcal specie, S. pseudintermedius, is more prevalent than S. aureus [4].…”

Section: Introductionmentioning

confidence: 98%

spa typing and antimicrobial resistance of Staphylococcus aureus from healthy humans, pigs and dogs in Tanzania

Katakweba

Muhairwa

Espinosa-Gongora

et al. 2016

J Infect Dev Ctries

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Introduction: Staphylococcus aureus is an opportunistic pathogen causing infections in humans and animals. Here we report for the first time the prevalence of nasal carriage, spa typing and antimicrobial resistance of S. aureus in a Tanzanian livestock community. Methodology: Nasal swabs were taken from 100 humans, 100 pigs and 100 dogs in Morogoro Municipal. Each swab was enriched in Mueller Hinton broth with 6.5% NaCl and subcultured on chromogenic agar for S. aureus detection. Presumptive S. aureus colonies were confirmed to the species level by nuc PCR and analysed by spa typing. Antimicrobial susceptibility patterns were determined by disc diffusion method. Results: S. aureus was isolated from 22 % of humans, 4 % of pigs and 11 % of dogs. A total of 21 spa types were identified: 13, 7 and 1 in human, dogs, and pigs, respectively. Three spa types (t314, t223 and t084) were shared between humans and dogs. A novel spa type (t10779) was identified in an isolate recovered from a colonized human. Antimicrobials tested revealed resistance to ampicillin in all isolates, moderate resistances to other antimicrobials with tetracycline resistance being the most frequent. Conclusion: S. aureus carrier frequencies in dogs and humans were within the expected range and low in pigs. The S. aureus spa types circulating in the community were generally not shared by different hosts and majority of types belonged to known clones. Besides ampicillin resistance, moderate levels of antimicrobial resistance were observed irrespective of the host species from which the strains were isolated.

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Antimicrobial Resistance of Staphylococcus aureus Strains Acquired by Pig Farmers from Pigs

Cited by 67 publications

References 41 publications

The effect of anatomic site and age on detection of Staphylococcus aureus in pigs

The effect of anatomic site and age on detection of Staphylococcus aureus in pigs

Carriage of Staphylococcus aureus by Free-Living Wild Animals in Spain

spa typing and antimicrobial resistance of Staphylococcus aureus from healthy humans, pigs and dogs in Tanzania

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