Antimicrobial resistance patterns of MDR Staphylococcus aureus clinical isolates involved in the lower respiratory tract infections in Egypt

Abstract

Resistance of Staphylococcus (S.) aureus to the currently used antimicrobials has risen dramatically in the past years creating a medical challenge as therapeutic options became very limited. This study aimed to screen and detect the prevalence of some antimicrobial-resistant genes of S. aureus clinical isolates recovered from patients suffering lower respiratory tract infections (LRTI) in Egypt. A total of 231 bacterial isolates were recovered from sputum and bronchoalveolar lavage specimens obtained from patients with LRTI. Thirty-seven isolates (16%) were identified as S. aureus where seventeen isolates (46%) showed resistance to ten or more antimicrobials. The antimicrobial susceptibility testing revealed that all the tested isolates were sensitive to vancomycin and linezolid (0%), however, the lowest resistance was observed to doxycycline (3%), and the highest resistance was observed to ciprofloxacin (51%). Sixteen isolates (43%) were found resistant to cefoxitin and harbored the mecA gene (100%). However, the mepA gene was detected in only 12 isolates (75%). Extended-spectrum β-lactamase (ESBL) including, ctx-m, shv and tem and the aac(6')-Ib genes, were detected in 10 (62%) and 8 (50%) isolates, respectively. None of the carbapenem-resistant genes including kpc, imp, vim, ndm, and oxa, were detected in any isolate. Multiple drug resistance (MDR) is a major health concern limiting the use of common antimicrobials in therapy. Thus, new national guidelines, as well as infection control strategies including antibiotic stewardship, must be implemented in the Egyptian hospitals to limit further spread of antimicrobial resistance.

Preparation and evaluation of a new combined conjugated vaccine against Klebsiella pneumonia and Pseudomonas aeruginosa

“…Pneumonia represents a high reason for morbidity, mortality, hospitalization, and antibiotic use in people of all ages, especially among children and adults above 65 years old (Agmy et al, 2013; Guillamet et al, 2016). It is considered the fifth leading reason for worldwide deaths (Abdelaziz et al, 2019). Furthermore, it is the leading cause of death among infectious diseases and a global cause of death, accounting for 12.8% of deaths worldwide (Liu et al, 2016), with a high prevalence in South Asia and sub‐Saharan Africa, as stated by the World Health Organization (WHO, 2019).…”