Antimicrobial silver nanoparticle induces organ deformities in the developing Zebrafish (Danio rerio) embryos

Abstract

Silver Nanoparticles were synthesized by Escherichia coli using Silver nitrate in the growth medium and characterized in X-Ray Diffraction, UVVis Spectrophotometer and Scanning Electron Microscope. They exhibited antimicrobial activity against human pathogens except Escherichia Coli. Nanoparticles were impregnated in yarn and analyzed for their inhibition in the broth culture. The Minimal Inhibitory Concentratio was calculated for the human pathogens in Microtitre plate. The toxicity assessment of the nanoparticles in the embryonic Zebrafish showed many organogensis deformities like cardiac malformations, eye and head edema, tail and trunk flexure were observed in the organ system of the developing embryos for 1 to 5 day post fertilization in different concentrations of Ag Nanoparticles. The Organogenesis disruptive effects were found in 14 -20 ng/ml of silver nanoparticles but the inhibition was found in 4-10ng/ml for the pathogens in vitro and 10ng/ml in embryos. Nevertheless, in Cardiac assay, the Heart Beat rates were calculated as 42 -45 for 15 Sec in the concentrations ranging from 10 -20 ng/ml of Silver nanoparticles. The blood flows, rhythmicity, contractility of heart beat rates were observed normal. The Mean value of blood Cell counting did not showed any notable effects in the Nanoparticle treated Zebrafish embryos and control. The LC50 value for the Biosynthesized nanoparticle was at 22 ng/ml in all the developmental stages of the embryos. Our results shows silver nanoparticles disrupts the normal organogenesis during development and further detailed studies are needed to prove silver nanopartcles are an antimicrobial agent for use in humans.

Effect of silver nanoparticles on maturation of rabbit’s oocytes co-cultured with granulosa cells in vitro

“…3, B). Although the concentrations of nanoparticles in this study was low (0.01-10 µg/ml), but lower concentrations of AgNPs 14-20 ng/ml showed toxic effects on embryos development in Zebrafish embryos [16]. Our results showed that silver nanoparticles with PVP and BSA at the concentration of 10 µg/ml inhibited granulosa cells proliferation, but did not influence oocytes maturation to metaphase-2 ( Fig.…”

Cynodon dactylon and Sida acuta extracts impact on the function of the cardiovascular system in zebrafish embryos

“…Embryos were raised in 10 mmol/L HEPES-buffered E3 medium in a dark incubator at 28°C until 60 hpf. Eggs containing dead or obviously weak embryos were removed[18],[19]. The study protocol was approved by the local institutional review board at the authors' affiliated institution and animal study was carried out in strict accordance with the established institutional guidelines.…”

Rapid neurobehavioural analysis based on the effects of an acetylcholinesterase inhibitor from Tephrosia purpurea in Zebrafish

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