Antimicrobial susceptibilities and population structure of Staphylococcus epidermidis associated with ovine mastitis

Onni, Toniangelo; Sanna, Giovanna; Larsen, Jesper; Tola, Sebastiana

doi:10.1016/j.vetmic.2010.07.024

Cited by 41 publications

(39 citation statements)

References 22 publications

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“…However, different antimicrobial susceptibilities and diverse virulence factors were also found in CNS species isolated from mastitis in dairy cows ); this may lead to ineffective treatment when using common antibiotics. In addition, an increasing number of CNS has been reported to be resistant to neomycin, penicillin, tetracycline, streptomycin, lincomycin, and ampicillin (Bochniarz and Wawron, 2011;Onni et al, 2011). Further, MDR isolates of S. epidermidis have been identified in the milk from both cows (Sawant et al, 2009;Waller et al, 2011) and women (Delgado et al, 2009), making these isolates a significant threat to public health.…”

Section: Discussionmentioning

confidence: 99%

Potential of berberine to enhance antimicrobial activity of commonly used antibiotics for dairy cow mastitis caused by multiple drug-resistant Staphylococcus epidermidis infection

Zhou¹,

Yang²,

Li³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Berberine is a plant alkaloid with antimicrobial activity against a variety of microorganisms. In this study, the antimicrobial properties of berberine against multi-drug resistant field isolates of Staphylococcus epidermidis were investigated using berberine alone or in combination with a commonly used antibiotics in veterinary clinics, including penicillin, lincomycin, and amoxicillin. The results indicated that the minimum inhibitory concentrations of berberine, penicillin, lincomycin, and amoxicillin against field S. epidermidis isolates were 2-512, 0.8-213, 0.4-1024, and 0.4-256 mg/mL, respectively. Furthermore, the synergistic effects of antimicrobial activity against these multi-drug resistant isolates were observed when the berberine was combined with penicillin, lincomycin, or amoxicillin; no antagonistic effect of the combination was detected in any of the clinical isolates.X. Zhou et al. 9684©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (3): 9683-9692 (2015) These observations were further confirmed using a time-killing assay, in which a combination of 2 agents yielded a greater than 2.03-2.44 log 10 decrease in colony-forming unit/mL compared with each agent alone. These findings suggest that berberine is a promising compound for preventing and treating multi-drug resistant S. epidermidis infected mastitis in dairy cows either alone or in combination with other commonly used antibiotics, such as penicillin, lincomycin, and amoxicillin.

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Section: Discussionmentioning

confidence: 99%

Potential of berberine to enhance antimicrobial activity of commonly used antibiotics for dairy cow mastitis caused by multiple drug-resistant Staphylococcus epidermidis infection

Zhou¹,

Yang²,

Li³

et al. 2015

Genet. Mol. Res.

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“…Although a recent study (Onni et al 2010) reported multi-drug resistance mediated by the mecA gene in S. epidermidis isolated from dairy sheep herds, methicillin resistant Staphylococcus spp. are considered unusual in veterinary medicine (Guérin-Faublée et al 2003).…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial resistance of Staphylococcus spp. from small ruminant mastitis in Brazil

et al. 2012

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Pesq. Vet. Bras. 32(8) The study aimed to determine the antimicrobial resistance patterns and to identify molecular resistance markers in Staphylococcus spp. (n=210) isolated from small ruminant mastitis in Brazil. The antimicrobial resistance patterns were evaluated by the disk diffusion test and by detection of the presence of mecA, blaZ, ermA, ermB, ermC and msrA genes by PCR. The efϐlux pump test was performed using ethidium bromide and bioϐilm production was determined by Congo red agar test along with PCR for detection of the icaD gene. The isolates were most resistant to amoxicillin (50.0%), streptomycin (42.8%), tetracycline (40.4%), lincomycin (39.0%) and erythromycin (33.8%). Pan-susceptibility to all tested drugs was observed in 71 (33.8%) isolates and 41 Staphylococcus isolates were positive for the efϐlux pump. Although phenotypic resistance to oxacillin was observed in 12.8% of the isolates, none harbored the mecA gene. However, 45.7% of the isolates harbored blaZ indicating that beta-lactamase production was the main mechanism associated with staphylococci resistance to beta-lactams in the present study. The other determinants of resistance to antimicrobial agents ermA, ermB, ermC, and msrA were observed in 1.4%, 10.4%, 16.2%, and 0.9% of the isolates, respectively. In addition, the icaD gen was detected in 32.9% of the isolates. Seventy three isolates (54 from goats and 19 from sheep) were negative for all resistance genes tested and 69 isolates presented two or more resistance genes. Association among blaZ, ermA, ermB, ermC and efϐlux pump were observed in 17 isolates, 14 of which originated from goats and three from sheep. The data obtained in this study show the resistance of the isolates to beta-lactamics, which may be associated with the use of antimicrobial drugs without veterinary control.

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“…In a study conducted in France, Even et al (2010) isolated 33 strains of Staphylococcus epidermidis from cheese and dry fermented sausages samples, and clinical samples from hospitalized patients, and reported that 73% were resistant to at least one of the antimicrobials: 9.18% to tetracycline, 36% to erythromycin, 39% to penicillin, and 18% to norfloxacin, among other antimicrobial agents. Onni et al (2011), in a study of 131 strains of S. epidermidis isolated from mastitis in goats reared in different geographical areas of the region of Sardinia, Italy, found that 38% of the strains were resistant to penicillin, 7.6% were resistant to tetracycline, and 2.3% resistant to both penicillin and tetracycline.…”

Section: Antimicrobial Resistance Of Bacterial Strains Isolated From mentioning

confidence: 99%

Antimicrobial resistance of bacterial strains isolated from avian cellulitis

Santos

Alcântara

Perecmanis

et al. 2014

Rev. Bras. Cienc. Avic.

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Avian cellulitis is an inflammatory process in the subcutaneous tissue, mainly located in the abdomen and thighs. This problem is commonly observed in poultry at slaughter and it is considered one of the major causes of condemnation of carcasses in Brazil. The aim of this study was to perform the microbial isolation of lesions of avian cellulitis from a processing plant located in the State of Goiás in order to analyze antimicrobial resistance by antibiogram test and to detect resistance genes by polymerase chain reaction. A total of 25 samples of avian cellulitis lesions were analyzed, from which 30 bacterial strains were isolated. There were eleven (44%) strains of Escherichia coli, nine (36%) strains of Staphylococcus epidermidis, seven (28%) strains of Proteus mirabilis and three (12%) strains of Manheimiahaemolytica. The antibiogram test showed that all strains were resistant to at least one antimicrobial. The gene of antimicrobial resistance tetB was detected in E. coli, S. epidermidis and P. mirabilis strains, and was the most frequently observed gene. The gene of antimicrobial resistance Sul1 was detected in all bacterial species, while tetA was found in E. coli and S. epidermidis strains, SHV in E. coli strains, S. epidermidis and P. mirabilis,and cat1 in one P. mirabilis strain. The results suggest a potential public health hazard due to the ability of these microorganisms to transmit antimicrobial resistancegenes to other microorganisms present in the intestinal tract of humans and animals, which may affect clinical-medical usage of these drugs. INTRODUCTIONAvian cellulitis was first described in Britain by Randall et al. (1984). Characterized as an acute purulent inflammation of the subcutaneous tissue, it is routinely encountered in the abdominal region and the legs of poultry (Messier et al. 1993, Gross 1994. Carcasses presenting cellulitis should be partially or totally condemned, as determined by Ordinance No.210 of 10/11/1998 of the Brazilian Ministry of Agriculture (Brazil, 1998), which establishes the Technical Regulations of Technological and Sanitary-Hygienic Inspection of Poultry Meat. Some authors mention that condition as one of the main causes of carcass condemnation in broiler processing plants (Brito et al., 2002;Armendaris, 2006;Santana et al., 2008). Considering the importance of this pathology in the context of poultry meat production, this study aimed at performing microbiological isolation, testing antimicrobial resistance, and detecting antimicrobial resistance genes in microorganisms isolated from avian cellulitis lesions obtained from chicken carcasses stored in the refrigerator of a processing plant located in the state of Goiás, Brazil.14 Santos MM, Alcântara ACM, Perecmanis S, Campos A, Santana AP

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Antimicrobial susceptibilities and population structure of Staphylococcus epidermidis associated with ovine mastitis

Cited by 41 publications

References 22 publications

Potential of berberine to enhance antimicrobial activity of commonly used antibiotics for dairy cow mastitis caused by multiple drug-resistant Staphylococcus epidermidis infection

Potential of berberine to enhance antimicrobial activity of commonly used antibiotics for dairy cow mastitis caused by multiple drug-resistant Staphylococcus epidermidis infection

Antimicrobial resistance of Staphylococcus spp. from small ruminant mastitis in Brazil

Antimicrobial resistance of bacterial strains isolated from avian cellulitis

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