Antimicrobial Susceptibility and Clonal Relation Between Acinetobacter baumannii Strains at a Tertiary Care Center in Turkey

Abstract

Background:Acinetobacter baumannii is an opportunistic pathogen, related with nosocomial infections such as bacteremia, urinary tract infections, and ventilator-associated pneumonia. Multidrug resistant (MDR) A. baumannii strains are first line causes of infection, especially in patients hospitalized at intensive care units (ICUs). Infection with MDR A. baumannii strains has a longer duration at ICUs and hospitals. There are studies using molecular methods which can differentiate MDR A. baumannii strains at the clonal level. This helps controlling these resistant strains and prevents their epidemy.Objectives:The aim of our study was to investigate the antimicrobial susceptibility and clonal relationship between the A. baumannii strains isolated from our ICU.Materials and Methods:The identification and antimicrobial susceptibility of 33 A. baumannii strains were performed by automatized Vitek version 2.0. The clonal relationship among A. baumannii strains was analyzed using enterobacterial repetitive intergenic consensus (ERIC) polymerase chain reaction (PCR).Results:A total of 33 A. baumannii strains were included in this study. A. baumannii complex strains were classified into seven clusters based on the fingerprint results. Our results revealed that two main clusters were responsible for the prevalence of A. baumannii complex strains at the ICU.Conclusions:MDR A. baumannii strains cause an increment in morbidity and mortality, particularly in ICUs. The use of molecular epidemiological methods can help us with the detection of the pathogen and preventing from spreading of these resistant strains.

Distribution and Genotyping of Aquatic Acinetobacter baumannii Strains Isolated from the Puzi River and Its Tributaries Near Areas of Livestock Farming

“…Despite MLST and PFGE being highly discriminative genotyping methods, PCR-based DNA fingerprinting techniques have advantages of performance ease and economic viability [2,6,12]. To date, there have been many studies describing different PCR-based methods to type MDR-AB [47][48][49]. In general, the ERIC-PCR method is a common, easy, and quick fingerprinting technique for characterising A. baumannii isolates [2,48,49].…”

Characterization of Oxacillinase and Metallo-β-Lactamas Genes and Molecular Typing of Clinical Isolates of Acinetobacter baumannii in Ahvaz, South-West of Iran

“…Clonal dissemination of carbapenem resistant A. baumannii was previously reported in different studies. It has been establish that multidrug resistant of A. baumannii isolates with similar genotype can disseminate among various wards, different hospitals and even among cities ( 4 , 10 , 12 , 49 - 51 ). In the current study, the vast majority of carbapenem resistant isolates (94/97, 96.9%) belonged to one of the four dominant genotypes indicating clonal dissemination of resistant isolates in the studied hospitals.…”

Evaluation of Risk and Prognostic Factors of Carbapenem Resistant Acinetobacter Baumannii Infections in Intensive Care Unit.

“…For this reason, it can cause severe infections in all settings of hospitals especially in intensive care units. Besides the periods in which the compliance with hand hygiene and standard isolation procedures decrease, application of mechanical ventilation to patients, surgical operation, application of invasive procedures, immunosuppressive treatment regimens and long stays give rise to an increase in MDR A. baumannii colonization and infection [4].…”