Antimicrobial, α-Glucosidase and Alkaline Phosphatase Inhibitory Activities of Bergenin, The Major Constituent of Cissus populnea Roots

Nyemb, Jean Noël; Djankou, Madeleine Tchoffo; Talla, Emmanuel; Tchinda, Alembert T.; Ngoudjou, David T; Iqbal, Jamshed; Mbafor, Joseph T

doi:10.4172/2161-0444.1000492

Cited by 9 publications

(9 citation statements)

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“…Pavithra et al [ 14 ] reported that methanol extracts of flowers from four angiosperm plant species, W. thyrsoidea , O. dioica , L. speciosa, and B. malabaricum hadn’t inhibitory effect against C. albicans . These results suggest that further studies should be carried out in order to determine a good inhibitory concentration, and this observation was also made by Nyemb et al [ 123 ].…”

Section: Biological and Pharmacological Activities Of Bergeninsupporting

confidence: 76%

“…(−), E. phaeacalis (+) and S. aureus (+) (IC 50 ranging from 3.125 to 6.25 µg/mL) [ 122 ]. Nyemb et al [ 123 ] reported that bergenin isolated from C. populnea roots had an inhibitory effect against four strains of Gram-negative bacteria in vitro: S. typhi (ATCC6539), S. typhi (isolated), P. aeruginosa (ATCC9721) and E. coli (isolated), IC 50 ranging from 8 to 64 µg/mL. These results suggest that bergenin is able to easily cross the complex and multilayer lipopolysaccharide cell walls of Gram-negative bacteria.…”

Section: Biological and Pharmacological Activities Of Bergeninmentioning

confidence: 99%

“…These results suggest that bergenin is able to easily cross the complex and multilayer lipopolysaccharide cell walls of Gram-negative bacteria. In doing so, it weakens them, causing lysis and consequent death [ 123 ]. However, some studies with bergenin had negative results [ 124 , 125 ].…”

Section: Biological and Pharmacological Activities Of Bergeninmentioning

confidence: 99%

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Chemistry and Pharmacology of Bergenin or Its Derivatives: A Promising Molecule

et al. 2023

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Bergenin is a glycosidic derivative of trihydroxybenzoic acid that was discovered in 1880 by Garreau and Machelart from the rhizomes of the medicinal plant Bergenia crassifolia (currently: Saxifraga crassifolia—Saxifragaceae), though was later isolated from several other plant sources. Since its first report, it has aroused interest because it has several pharmacological activities, mainly antioxidant and anti-inflammatory. In addition to this, bergenin has shown potential antimalarial, antileishmanial, trypanocidal, antiviral, antibacterial, antifungal, antinociceptive, antiarthritic, antiulcerogenic, antidiabetic/antiobesity, antiarrhythmic, anticancer, hepatoprotective, neuroprotective and cardioprotective activities. Thus, this review aimed to describe the sources of isolation of bergenin and its in vitro and in vivo biological and pharmacological activities. Bergenin is distributed in many plant species (at least 112 species belonging to 34 families). Both its derivatives (natural and semisynthetic) and extracts with phytochemical proof of its highest concentration are well studied, and none of the studies showed cytotoxicity for healthy cells.

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Section: Biological and Pharmacological Activities Of Bergeninsupporting

confidence: 76%

Section: Biological and Pharmacological Activities Of Bergeninmentioning

confidence: 99%

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Chemistry and Pharmacology of Bergenin or Its Derivatives: A Promising Molecule

et al. 2023

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“…Noteworthy, bergenin and its congeners participate in several mechanisms, such as lipid-peroxidation inhibitory activity and free-radical-scavenging activity [38], and initiate apoptosis and cell-cycle arrest in the G0/G1 phase [39]. Moreover, these compounds are able to easily cross the complex and multilayer lipopolysaccharide of bacteria cell walls, thereby causing lysis and cell death [40]. Thus, it is not unreasonable to speculate that these compounds might have contributed to the anti-shigellosis activity.…”

Section: Discussionmentioning

confidence: 99%

Anti-Shigellosis Activity and Mechanisms of Action of Extracts from Diospyros gilletii Stem Bark

Nguelo Talla,

Madiesse Kemgne,

Ngouana

et al. 2024

DDC

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Shigellosis is a pathological condition that affects the digestive system and possibly causes diarrhoea. Shigella species, which are responsible for this disease, are highly contagious and spread through contaminated food and water. The increasing development of resistance by Shigella species necessitates the urgent need to search for new therapies against diarrhoea-causing shigellosis. The scientific validation of medicinal plants, such as Diospyros gilletii, which is used for the traditional treatment of diarrhoeal conditions is worthwhile. The present study aims to investigate the antibacterial activity of extracts from D. gilletii against selected Shigella species. Extracts from D. gilletii stem bark were prepared by maceration using various solvents. The antibacterial activity of D. gilletii extracts was evaluated in Shigella dysenteriae, S. flexneri, S. boydii, and S. sonnei using a microdilution method, whereas a cytotoxicity test was performed on Vero and Raw cells using resazurin-based colorimetric assays. Bacterial membrane-permeability studies were evaluated using propidium iodide (PI)- and 1-N-phenyl-naphthylamine (NPN)-uptake assays, whereas inhibition and eradication tests on bacterial biofilms were carried out by spectrophotometry. As a result, methanol, ethanol and hydroethanol (water: ethanol; 30:70, v/v) extracts of D. gilletii inhibited the growth of S. boydii, S. flexneri and S. sonnei, with minimum inhibitory concentration (MIC) values ranging from 125 to 500 µg/mL, without toxicity to Vero and Raw cells. Time-kill kinetics revealed bactericidal orientation at 2 MIC and 4 MIC and a bacteriostatic outcome at 1/2 MIC. The mechanistic basis of antibacterial action revealed that D. gilletii extracts inhibited and eradicated Shigella biofilms and promoted the accumulation of NPN and PI within the inner and outer membranes of bacteria to increase membrane permeability, thereby causing membrane damage. This novel contribution toward the antibacterial mechanisms of action of D. gilletii extracts against Shigella species substantiates the use of this plant in the traditional treatment of infectious diarrhoea.

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“…Saponin was found to be highest in the stem bark [16]. Bergenin, daucosterol, stigmasterol and β-sitosterol have been isolated from the root of C. populnea [17]. Also, Danladi et al [8] reported the isolation of β-sitosterol from the leaf of C. populnea (Figure 2).…”

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of Antimicrobial Constituent(s) from the Stem of Cissus populnea Guill. & Perr.

Alex-Asaolu,

Uba,

Birnin-Yauri

et al. 2024

DDC

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Cissus populnea Guill. & Perr. (Vitaceae) is used in traditional medicine to treat microbial infections, venereal diseases and infertility, among others. The aim of this research is to isolate and characterize the antimicrobial constituent(s) from the stem of C. populnea. The n-butanol fraction of C. populnea, being most active, was subjected to silica gel column chromatography, which led to the isolation of white solid and white crystalline substances coded compounds C1 and C4C5, respectively. Spectral analysis (1D and 2D-NMR) of the isolated compounds and comparison with the literature data indicated C1 to be Bis-(2-ethyloctyl)-phthalate and C4C5 to be a mixture of stigmasterol and β-sitosterol; C4C5 exhibited a zone of inhibition ranging from 24 to 29 mm against the test organisms with Candida albicans being the most sensitive organism while Trichophyton rubrum was the least sensitive organism. Of the standard drugs, ciprofloxacin had 27–37 mm while fluconazole and fulcin exhibited zones of inhibition ranging from 34 mm to 29–32 mm, respectively. The MIC and MBC/MFC values for C4C5 ranges from 12.5 to 25.0 µg/cm3 and 25.0 to 50.0 µg/cm3 against methicillin-resistant Staphylococcus aureus, Staphylococcus. aureus, Escherichia coli, Candida albicans, Trichophyton rubrum and Trichophyton mentagrophyte, respectively. In conclusion, Bis-(-(2-ethyloctyl)-phthalate) and a mixture of stigmasterol and β-sitosterol were identified for the first time from the stem of C. populnea.

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Antimicrobial, α-Glucosidase and Alkaline Phosphatase Inhibitory Activities of Bergenin, The Major Constituent of Cissus populnea Roots

Cited by 9 publications

References 0 publications

Chemistry and Pharmacology of Bergenin or Its Derivatives: A Promising Molecule

Chemistry and Pharmacology of Bergenin or Its Derivatives: A Promising Molecule

Anti-Shigellosis Activity and Mechanisms of Action of Extracts from Diospyros gilletii Stem Bark

Isolation and Characterization of Antimicrobial Constituent(s) from the Stem of Cissus populnea Guill. & Perr.

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