Antimicrobials in beekeeping

Reybroeck, Wim; Daeseleire, Els; Brabander, H.F. De; Herman, Lieve

doi:10.1016/j.vetmic.2012.01.012

Cited by 136 publications

(108 citation statements)

References 55 publications

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“…The untreated foul brood disease not only destroys the hive bee population, but also wipes out an apiary because colonies which showed no visible AFB signs may be infected but not yet showing signs of disease. [21] Such a finding is surprising as this was the area where the prevalence of AFB was not expected by beekeepers for several years. The higher level of AFB incidents in district Karak and Bannu (Figure 2) may be due to migratory beekeeping activities in these regions as it has become one of the strongest berry honey production areas in the province of Khyber Pakhtunkhwa (Figure 1).…”

Section: Sequencing Of 16s Ribosomal Dnamentioning

confidence: 96%

Prevalence of American foul brood disease of honeybee in north-west Pakistan

Anjum

Shah

Azim

et al. 2015

Biotechnology & Biotechnological Equipment

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American foul brood (AFB) disease is a deleterious bacterial disease worldwide, caused by spore forming bacterium Peanibacillus larvae that affects honeybee larvae and causes a significant decrease in the honeybee population. Following symptomatical and bacteriological approaches combined with 16S rDNA sequencing, an assessment has been made to evaluate the presence of AFB disease in North-West Pakistan as no record for bee-associated bacterial disease from Pakistan is available. A total of 1276 samples from 1520 bee colonies (15 apiaries) were collected, of which 476 samples (37.30%) were found with symptoms of AFB. Biochemical and 16S rDNA analysis indicated that all these farms have Peanibacillus larvae infection. It is concluded that the prevalence of AFB bacterial disease to such an extent in these regions of Pakistan will devastate the apicultural industries in a large scale across the country.

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Section: Sequencing Of 16s Ribosomal Dnamentioning

confidence: 96%

Prevalence of American foul brood disease of honeybee in north-west Pakistan

Anjum

Shah

Azim

et al. 2015

Biotechnology & Biotechnological Equipment

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“…The handling of unbranded honey is usually poor and does not look presentable but have heath benefits. The extensive use of antibiotics in beekeeping has led to an accumulation of antibiotic residues in honey, thereby leading to decreased quality and difficulty in marketing (Tillotson et al, 2006;Sapna and Nimisha 2010;Orianne et al, 2013;Reybroeck et al, 2012;Mahmoudi et al, 2014). Antibiotics are used in beekeeping as growth promoters and to treat diseases.…”

Section: Honey Is a Sweet Viscous Liquid Produced By Honey Beementioning

confidence: 99%

Antibacterial potential components of Bacillus species and antibiotics residues in branded and unbranded honey samples from Nigeria

Agbagwa¹,

Rani²,

Halami³

2017

Afr. J. Biotechnol.

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Honey is a sweet viscous liquid produced by honey bee, Apis mellifera from the nectar of plants. Honey is a natural product that has been used from ancient times till now as food and for medicinal purpose. This study was carried out to determine the mode of action of Bacillus species and antibiotics residues in branded and unbranded honey samples from Nigeria. Bacilli spp. count was carried out by initially heating diluted honey samples in water bath set at 80°C for 15 min, while total bacterial count was carried out using the pour plate method. Antibacterial activity of identified Bacillus spp on Micrococcus was determined using well-in agar method while the mode of action was carried out by reporter assay method. Detection of tetracycline, gentamycin and streptomycin was analyzed using high performance liquid chromatography (HPLC) column oven L-2300 and Column intensil ODS-3C18 (250 x 4.6 mm). Honey samples (2 g) were extracted for HPLC by deprotenizing using acetonitrile and methanol with flow rate of 1 mL/min and RID detector was used to detect antibiotic residue. Bacilli from honey were characterized physiologically, morphologically and biochemically, they were tentatively identified as Bacilli licheniformis, Bacilli subtilis, Bacilli coagulans, Bacilli cirulans, Bacilli pumilis and Bacilli badius. The most prevalent Bacillus spp. were B. licheniformis and B. subtilis. Total bacteria count for branded honey ranged from 2.2 x 10 2 to 5.5 x 10 3 cfu/g, while Bacilli count ranged from nil to 6.2 x 10 2 cfu/g. For unbranded honey samples, total bacteria count ranged from 7.0 x 10 3 to 3.5 x 10 2 cfu/g, while Bacilli count ranged from 5 x 10 1 to 1.6 x 10 3 cfu/g. Four of the isolates representing branded (SF2 and RW2) and unbranded honey samples (EH2 and TC2) exhibited antibacterial activity against Micrococcus; one isolate (SF2) showed cell wall causing antibacterial activity. Tetracycline was detected more in the unbranded honey samples while gentamycin and streptomycin were detected in just two unbranded honey samples, indicating that tetracycline is used frequently for the treatment of bee diseases that is why it is detected as residue in the finished honey product.

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“…Antibiotics have been used to keep the disease in control. But, the antibiotics can't eliminate the disease exactly (Reybroeck et al, 2012). Also, the use of antibiotics in the treatment of bee diseases has been banned in some countries (Genersch, 2010).…”

Section: Introductionmentioning

confidence: 99%

Plant Essential Oils Used Against Some Bee Diseases

Tutun

Koç

Kart

2018

Turkish JAF Sci.Tech.

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The most common honey bee diseases are American foulbrood (AFB) caused by the bacterium Paenibacillus larvae, Chalkbrood caused by fungus Ascosphaera apis and diseases caused by parasitic mites such as Acarapis woodi, Varroa destructor. These diseases and pests not only cause economic loss but also cause ecological problems related to the role of honey bees, as the most important pollinators on Earth. Synthetic acaricides and antibiotics are used to keep the diseases and mites in control. Use of the drugs lead to the development of drug-resistant organisms, detrimental effect on non-target organisms and the residue problem in bee products. For this reasons, the need for alternative control methods has become compulsory in recent years. It has been known that some plant oils used widely in perfumery and food industry for flavor and smell have been used as repellent to certain insects, bactericide and fungicide. Therefore, intensive studies have been carried out on plants with anti-mites, antibacterial and antifungal potentials and these studies are still going on. Recently, studies in this area have shown that essential oils of plants such as thyme, cloves, mint, lemon grass, cinnamon, grapefruit, rosemary, marigold, are lethal to some mites, bacteria and fungi. In addition, it has been reported that some components, isolated from these plants such as sanguinarine, thymoquinone, capsaicin, carvacrol, citral, eugenol, thymol, show these effects on the organisms. As a result, in countries rich in biodiversity due to endemic plant species, the essential oils used in control of these diseases should be favored instead of or in combination with conventional drugs in integrated the disease management programs because of the lack of harmful effects of essential oils on non-target organisms and environment.

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Antimicrobials in beekeeping

Cited by 136 publications

References 55 publications

Prevalence of American foul brood disease of honeybee in north-west Pakistan

Prevalence of American foul brood disease of honeybee in north-west Pakistan

Antibacterial potential components of Bacillus species and antibiotics residues in branded and unbranded honey samples from Nigeria

Plant Essential Oils Used Against Some Bee Diseases

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