Antimitotic Activity on Sea Urchin Embryonic Cells of Seven Antiparasitic Morita-Baylis-Hillman Adducts: A Potential New Class of Anticancer Drugs

Leite, Jocelmo C. A.; Claudio, G. L.; Silva, Fábio P.L.; Sousa, Suervy C.O.; Vasconcellos, Mário L. A. A.; Marques‐Santos, Luis Fernando

doi:10.2174/1573406411208061003

Cited by 3 publications

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A2CN (3-hydroxy-2-methylene-3-(4-nitrophenyl)-propanenitrile), A3CN (3-hydroxy-2-methylene-3-(3-nitrophenyl)-propanenitrile), and A4CN (3-hydroxy-2-methylene-3-(2-nitrophenyl)-propanenitrile) (>99% pure) were synthesized and provided as described previously [21]. A 20 mM stock solution of A2CN, A3CN, and A4CN was prepared with dimethyl sulfoxide (DMSO) and freshly diluted in culture media for all in vitro experiments and in the control condition, and cells were treated using only the vehicle.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Morita-Baylis-Hillman Adducts Display Anti-Inflammatory Effects by Modulating Inflammatory Mediator Expression in RAW264.7 Cells

Faheina–Martins

Leite

Dantas

et al. 2017

Mediators of Inflammation

View full text Add to dashboard Cite

Inflammatory response plays an important role not only in the normal physiology but also in pathologies such as cancers. The Morita-Baylis-Hillman adducts (MBHA) are a novel group of synthetic molecules that have demonstrated many biological activities against some parasitic cells such as Plasmodium falciparum, Leishmania amazonensis, and Leishmania chagasi, and antimitotic activity against sea urchin embryonic cells was also related. However, little is known about the mechanisms induced by MBHA in inflammatory process and its relation with anticancer activity. The present work investigated the cytotoxicity of three MBHA derivatives (A2CN, A3CN, and A4CN), on human colorectal adenocarcinoma, HT-29 cells, and their anti-inflammatory activities were examined in lipopolysaccharide- (LPS-) stimulated RAW264.7 macrophage cells, being these derivatives potentially cytotoxic to HT-29 cells. Coincubation with A2CN, A3CN, or A4CN and LPS in RAW264.7 cells inhibited NO production, as well as the production of reactive oxygen species (ROS) was also repressed. The mRNA expressions of IL-1β and IL-6 were significantly downregulated by such MBHA compounds in RAW264.7 cells, but only A2CN was able to inhibit the COX-2 gene expression. We also showed that MBHA compounds decreased almost to zero the production of IL-1β and IL-6. These findings display that such MBHA compounds exhibit anticancer and anti-inflammatory activities.

show abstract

Section: Methodsmentioning

confidence: 99%

“…The Morita-Baylis-Hillman adducts (MBHA) have been described as anticancer compounds [20–22]. It has been published that some MBHA molecules exhibited antimitotic activity against sea urchin embryonic cells [21, 22], and recently, our group demonstrated that these molecules have anticancer potential [23]. …”

Section: Introductionmentioning

confidence: 99%

Morita-Baylis-Hillman Adducts Display Anti-Inflammatory Effects by Modulating Inflammatory Mediator Expression in RAW264.7 Cells

Faheina–Martins

Leite

Dantas

et al. 2017

Mediators of Inflammation

View full text Add to dashboard Cite

show abstract

“…The sea urchin is an already well-established marine model organism for developmental biology studies, largely used in drug discovery research for the understanding of cell proliferation processes induced by natural compounds with antimitotic activity [ 18 , 19 ]. In the last two decades, many studies have demonstrated the activation, at the enzymatic and morphological level, of apoptosis in sea urchins [ 20 , 21 , 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

Diatom-Derived Polyunsaturated Aldehydes Activate Similar Cell Death Genes in Two Different Systems: Sea Urchin Embryos and Human Cells

Galasso

Celentano

Costantini

et al. 2020

IJMS

View full text Add to dashboard Cite

Programmed cell death, such as apoptosis and autophagy, are key processes that are activated early on during development, leading to remodelling in embryos and homeostasis in adult organisms. Genomic conservation of death factors has been largely investigated in the animal and plant kingdoms. In this study, we analysed, for the first time, the expression profile of 11 genes involved in apoptosis (extrinsic and intrinsic pathways) and autophagy in sea urchin Paracentrotus lividus embryos exposed to antiproliferative polyunsaturated aldehydes (PUAs), and we compared these results with those obtained on the human cell line A549 treated with the same molecules. We found that sea urchins and human cells activated, at the gene level, a similar cell death response to these compounds. Despite the evolutionary distance between sea urchins and humans, we observed that the activation of apoptotic and autophagic genes in response to cytotoxic compounds is a conserved process. These results give first insight on death mechanisms of P. lividus death mechanisms, also providing additional information for the use of this marine organism as a useful in vitro model for the study of cell death signalling pathways activated in response to chemical compounds.

show abstract

Growth and toxicity of Halomicronema metazoicum (Cyanoprokaryota, Cyanophyta) at different conditions of light, salinity and temperature

et al. 2019

View full text Add to dashboard Cite

Cyanobacteria may live in the water column and in the benthos of aquatic environments, or be symbionts of other organisms, as in the case of Phormidium-like cyanobacteria, known to influence the ecology of freshwater and marine ecosystems. A strain of Phormidium-like cyanobacteria has been recently isolated as a free-living epiphyte of leaves of Posidonia oceanica (L.) Delile in the Mediterranean sea and its biology and ecology are herein investigated. It was identified as Halomicronema metazoicum, previously known uniquely as a symbiont of marine sponges. We cultivated it in a range of light irradiances, temperatures and salinities, to establish the most suitable conditions for the production of allelopathic and toxic compounds. The bioactivity of its spent culture medium was measured by means of standard toxicity tests performed on two model organisms. Our results indicate that at least two bioactive compounds are produced, at low and high irradiance levels and at two temperatures. The main compounds influencing the survival of model organisms are produced at the highest temperature and high or intermediate irradiance levels. The present research contributes to the understanding of critical toxigenic relationships among cyanobacteria and invertebrates, possibly influencing the ecology of such a complex environment as P. oceanica. Future isolation, identification and production of bioactive compounds will permit their exploitation for biotechnologies in the field of ecological conservation and medical applications.

show abstract

Antimitotic Activity on Sea Urchin Embryonic Cells of Seven Antiparasitic Morita-Baylis-Hillman Adducts: A Potential New Class of Anticancer Drugs

Cited by 3 publications

References 0 publications

Morita-Baylis-Hillman Adducts Display Anti-Inflammatory Effects by Modulating Inflammatory Mediator Expression in RAW264.7 Cells

Morita-Baylis-Hillman Adducts Display Anti-Inflammatory Effects by Modulating Inflammatory Mediator Expression in RAW264.7 Cells

Diatom-Derived Polyunsaturated Aldehydes Activate Similar Cell Death Genes in Two Different Systems: Sea Urchin Embryos and Human Cells

Growth and toxicity of Halomicronema metazoicum (Cyanoprokaryota, Cyanophyta) at different conditions of light, salinity and temperature

Contact Info

Product

Resources

About