Antimitotic effect, G2/M accumulation, chromosomal and ultrastructure changes in meristematic cells of<i>Allium cepa</i>L. root tips treated with the extract from<i>Rhodiola rosea</i>roots

Majewska, Agnieszka; Wolska, Ewa; Śliwińska, Elwira; Furmanowa, M.; Urbańska, Natalia; Pietrosiuk, Agnieszka; Zobel, Alicja M.; Kuraś, Mieczysław

doi:10.1080/00087114.2003.10589343

Cited by 54 publications

(32 citation statements)

References 15 publications

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“…In addition it may be due to inhibition of DNA synthesis or blocking of G1, suppressing DNA synthesis or effect of test compound at G2 phase of the cell cycle (Sudhakar et al 2001;ElGhamery et al 2000;Majewska et al 2003). So, we can state the decline of MI in our study can be related to these reasons.…”

Section: Discussionmentioning

confidence: 59%

Potential cytotoxic effect of Anilofos by using Allium cepa assay

et al. 2014

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Cytogenetic effects of Anilofos which was widely used in agriculture, was evaluated in Allium cepa root meristematic cells. In the Allium root growth inhibition test EC 50 value was determined 50 ppm and 1/29 EC 50 (25 ppm), EC 50 (50 ppm) and 2 9 EC 50 (100 ppm) concentrations of Anilofos were applied to onion roots. A negative and positive control were used in the experiment in parallel. According to results mitotic index decreased with increasing the Anilofos concentrations in all application groups and each exposure time, while disturbed anaphase-telophase, choromosome laggard(s), stickiness and anaphase bridge(s) were observed. In anaphase-telophase cells, c-metaphase, disturbed nucleus and binuclear cells were observed in other anomalies. The results were also analyzed statistically by using Dunnett t test (2-tailed) and all concentrations of Anilofos were found significant.

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Section: Discussionmentioning

confidence: 59%

Potential cytotoxic effect of Anilofos by using Allium cepa assay

et al. 2014

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“…Jabee et al (2008) also reported that chromosome bridges may arise due to stickiness or due to formation of dicentric chromosome by breakage and reunion. Inhibition of cytokinesis following telophase is responsible for binucleated cell formation (Majewska et al 2003). Akaneme and Amaefule (2012) reported the occurrence of nuclear lesions which may be due to the inhibitory action of phytochemicals on DNA biosynthesis.…”

Section: Discussionmentioning

confidence: 99%

Genotoxicity assessment of two common curing weeds: Hyptis suaveolens (L.) Poir. and Leucas indica (L.) R. Br.

Sumitha

Thoppil

2015

Cytotechnology

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Hyptis suaveolens and Leucas indica, two common weeds were selected for the present study, to reveal their probable cytotoxic potential. The meristematic root tips of Allium cepa were used for testing the cytotoxic property of the aqueous leaf extracts containing both polar and non-polar compounds, and that containing polar compounds alone, at different concentrations (0.125, 0.25, 0.5, 1 and 2 %) and at different time durations, using distilled water as negative control. Mitotic squash preparations were made using a standard protocol. The mitotic index of the treated root tip cells was found to be decreasing and the abnormality percentage was found to be increasing with increase in extract concentration when compared with the control. Maximum cytotoxicity was observed in the extract containing both polar and non-polar compounds. Both the tested plants were found to be cytotoxic. The abnormalities noticed were of both clastogenic (nuclear lesions, nuclear fragmentation, etc.) and non-clastogenic (aberrant cell wall formation at cytokinesis, ball metaphase, etc.) types. Both plant extracts were found to significantly (P \ 0.05) inhibit root growth of Allium cepa with an EC 50 value of 1.92 % (R 2 = 0.594) for Hyptis and 1.58 % (R 2 = 0.757) for Leucas. The results were also analysed statistically by using analysis of variance followed by appropriate post hoc tests. These two weeds are aromatic plants comprising of essential oils that are volatile, natural complex compounds characterized by a strong odour and formed as secondary metabolites. In nature, essential oils play an important role in the protection of the plants as insecticides by reducing their appetite for such plants. When specifically targeted the concept of effectively exploiting these weeds for the formulation of herbal insecticides/ pesticides may be possible in the near future.

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“…Inhibition of cytokinesis following telophase was responsible for binucleated cell formation visible in the next interphase of a new cell cycle. In Allium cepa, some authors suggested that phragmoplast inhibition at the early stage of telophase is the responsible disturbance for binucleated cell formation (Rank et al, 2002;Majewska et al, 2003). Effects of pendimethalin and P. resinovorans E20 on leaf protein of V. faba plants Treatment of V. faba with each of the two treatments resulted in some changes in the leaf protein banding patterns as compared with those of the control.…”

Section: Effect Of Pendimethalin and P Resinovorans E20 Bacteria On mentioning

confidence: 99%

Degenotoxicity of Pendimethalin Contaminated Clay Soil by Pseudomonas resinovorans Using Anatomical, Cytogenetic and Biochemical Analysis in Vicia faba Plants

Aboulila¹,

Belal²,

Metwaly³

et al. 2016

Int. J. Curr. Res. Biosci. Plant Biol.

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A b s t r a c t A r t i c l e I n f oBiodegradation of the selective herbicide pendimethalin which is used to control most annual grasses was investigated in water and soil. A pendimethalin degrading bacterial isolate (designated strain E20) was isolated using enrichment technique from wheat soil previously treated with the herbicide pendimethalin on the basis of morphological, physiological and genetical (16s rDNA) tests, this bacterial isolate proved to be identified as Pseudomonas resinovorans E20. Results showed that the optimum pH and temperature for the growth of pendimethalin degrading strain were 7 and 30°C, respectively. Additional carbon sources (glucose, lactose, starch, carboxymethyl cellulose and phenol) decreased pendimethalin degradation. Pseudomonas resinovorans E20 was used to remove pendimethalin from aquatic system with half-life of 5.9 days. Pendimethalin halflife was 49.2 days in untreated mineral liquid medium as control. Pseudomonas resinovorans E20 was also evaluated for degradation of pendimethalin in clay soil with half-life of 5.004 days, its half-life was 66.9 days in untreated clay soil. The obtained results illustrated that pendimethalin treatment reduced all measured plant features (plant height, root length, xylem vessels diameter, thickness of phloem tissue, vascular bundle as well as vascular bundle width) in comparison to the other treatments. On the other side, treatment with Pseudomonas resinovorans E20 led to improving and increasing the above investigated parameters. Evaluation of the genotoxic effects of pendimethalin on the genetic material of Vicia faba plants revealed that, the highest abnormal mitosis percentage (63.28%) was recorded for pendimethalin treated plants. On the other hand, Vicia faba plants treated with the bacterial strain Pseudomonas resinovorans E20 decrease the abnormal mitosis percentage which reached to 21.35% in comparison with the control treatment which gave a percentage of 3.85%. This indicates the degenotoxic effect of this bacterial strain pseudomonas resinovorans E20. At the biochemical genetic level, the two treatments (pendimethalin and pseudomonas resinovorans E20) induced some changes in protein banding patterns of Vicia faba plants. These changes included the disappearance of three protein bands with MW′s of 265, 175 and 40 KDa after treatment with the herbicide pendimethalin alone and appearance of the same bands after treatment with Pseudomonas resinovorans E20 bacterial strain.

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Antimitotic effect, G2/M accumulation, chromosomal and ultrastructure changes in meristematic cells ofAllium cepaL. root tips treated with the extract fromRhodiola rosearoots

Cited by 54 publications

References 15 publications

Potential cytotoxic effect of Anilofos by using Allium cepa assay

Potential cytotoxic effect of Anilofos by using Allium cepa assay

Genotoxicity assessment of two common curing weeds: Hyptis suaveolens (L.) Poir. and Leucas indica (L.) R. Br.

Degenotoxicity of Pendimethalin Contaminated Clay Soil by Pseudomonas resinovorans Using Anatomical, Cytogenetic and Biochemical Analysis in Vicia faba Plants

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