Antinociceptive and Anti-Inflammatory Effects of Zerumbone against Mono-Iodoacetate-Induced Arthritis

Abstract

The fresh rhizome of Zingiber zerumbet Smith (Zingiberaceae) is used as a food flavoring and also serves as a folk medicine as an antipyretic and for analgesics in Taiwan. Zerumbone, a monocyclic sesquiterpene was isolated from the rhizome of Z. zerumbet and is the major active compound. In this study, the anti-inflammatory and antinociceptive effects of zerumbone on arthritis were explored using in vitro and in vivo models. Results showed that zerumbone inhibited inducible nitric oxide (NO) synthase (iNOS), cyclooxygenase (COX)-2 expressions, and NO and prostaglandin E2 (PGE2) production, but induced heme oxygenase (HO)-1 expression in a dose-dependent manner in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. When zerumbone was co-treated with an HO-1 inhibitor (tin protoporphyrin (SnPP)), the NO inhibitory effects of zerumbone were recovered. The above results suggest that zerumbone inhibited iNOS and COX-2 through induction of the HO-1 pathway. Moreover, matrix metalloproteinase (MMP)-13 and COX-2 expressions of interleukin (IL)-1β-stimulated primary rat chondrocytes were inhibited by zerumbone. In an in vivo assay, an acetic acid-induced writhing response in mice was significantly reduced by treatment with zerumbone. Furthermore, zerumbone reduced paw edema and the pain response in a mono-iodoacetate (MIA)-induced rat osteoarthritis model. Therefore, we suggest that zerumbone possesses anti-inflammatory and antinociceptive effects which indicate zerumbone could be a potential candidate for osteoarthritis treatment.

Carbonyl Activation in Electrophilic Polyene Cyclizations: A Toolbox for the Design of Isoprenoid Libraries

“…], [7] and the contribution of the transannularly conjugated dipolar formula 3 to its structure and chemistry, [8] made us wonder whether the presence of the carbonyl group could provide an electronic finesse and/or the necessary preorganization of the cycloundecatriene system to trigger aconcerted transannular cyclization and breach the Eolefin barrier. [9] Since 2 shows potent activity in animal models of human chronic degenerative diseases like cancer, [10] diabetes, [11] and arthritis, [12] and is also of relevance for perfumery, [13] the structural context for probing this concept was not only mechanistically appealing,but also relevant for medicinal and lifestyle related applications. [9] Since 2 shows potent activity in animal models of human chronic degenerative diseases like cancer, [10] diabetes, [11] and arthritis, [12] and is also of relevance for perfumery, [13] the structural context for probing this concept was not only mechanistically appealing,but also relevant for medicinal and lifestyle related applications.…”

“…[4] To avoid the formation of endocyclic E-configured double bonds in unsaturated rings smaller than cyclooctene,asituation topologically impossible, [5] the cyclization of 1,a na ll-E-configured cyclo-undecatriene,m ust follow ap athway that involves all three double bonds in ac oncerted fashion. [9] Since 2 shows potent activity in animal models of human chronic degenerative diseases like cancer, [10] diabetes, [11] and arthritis, [12] and is also of relevance for perfumery, [13] the structural context for probing this concept was not only Figure 1. [4] Ther eady availability of the 8-oxo derivative of ahumulene (zerumbone; 2)f rom at ropical ginger [Zingiber zerumbet (L.) Roscoe ex Sm.…”

Zerumbone promotes proliferation of endogenous neural stem cells in vascular dementia by regulating Notch signalling

“…Cerebral ischemia causes neuronal injury which leads to vascular dementia and the endogenous neuronal stem cell is not able to prevent the neuronal injury [3]. There are several research studies going on the protection of brain injury and still the repair of neuronal injury is a difficult task.…”

Phytochemical analysis and antinociceptive activity of bitter ginger (Zingiber zerumbet) cultivated in Manaus/Amazonas

“…Through this test it was possible to demonstrate, for the first time, that ZER administered via oral route, inhibits acetic acid induced writhing up to 98% within 2 h. In an in vivo assay, an acetic acid-induced writhing response in mice was significantly reduced by treatment with zerumbone. Furthermore, zerumbone reduced paw edema and the pain response in a mono-iodoacetate (MIA)-induced rat osteoarthritis model (Chien et al, 2016). Sulamain et al (2010) tested ZER in doses of 10, 50 and 100 mg/kg with realized inhibitions of 19.3, 40.4 and 64.8%, respectively as compared to the control (acetylsalicylic acid).…”