Antiosteoporotic activities of isoquercitrin in ovariectomized rats: Role of inhibiting hypoxia inducible factor-1 alpha

Fayed, Hadeer A.; Barakat, Bassant M.; Elshaer, Shereen Saeid; Abdel-Naim, Ashraf B.; Menze, Esther T.

doi:10.1016/j.ejphar.2019.172785

Cited by 37 publications

(28 citation statements)

References 45 publications

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“…Serum analyses of minerals, osteogenic markers and bone turnover markers revealed higher calcium, phosphorus, osterix, runt-related transcription factor 2 (Runx-2), bone formation markers (ALP and N-terminal propeptide of type 1 procollagen (P1NP)) and lower bone resorption markers (CTX and tartrate-resistant acid phosphatase (TRAP)) in the quercitrin-treated ovariectomised rats [36]. Fayed et al (2019) further delineated the anti-osteoporotic activities of isoquercitrin in ovariectomised rats. The highest dose of isoquercitrin applied in this study (60 mg/kg/day) significantly increased lumbar compression strength.…”

Section: Type Of Animalmentioning

confidence: 91%

“…The highest dose of isoquercitrin applied in this study (60 mg/kg/day) significantly increased lumbar compression strength. Bone turnover markers were reduced in the isoquercitrin-treated ovariectomised rats [ 37 ]. Isoquercitrin was used as previous studies indicated better bioavailability and higher antioxidative activity than quercetin and quercitrin [ 58 , 59 ].…”

Section: In Vivo Evidence On the Effects Of Quercetin On Bonementioning

confidence: 99%

“…The suppression of osteoclastic differentiation and formation induced by flavonoid quercetin is mediated through the inhibition of NF-κB and AP-1 activation [ 74 , 96 ]. In femur bone sections from ovariectomised rats treated with isoquercitrin, immunohistochemical staining of NF-κB in osteoclasts showed lower density than in untreated negative controls [ 37 ].…”

Section: The Underlying Mechanisms Of Action Of Quercetin As a Bonmentioning

confidence: 99%

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Quercetin as an Agent for Protecting the Bone: A Review of the Current Evidence

Wong

Chin

2020

IJMS

143

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Quercetin is a flavonoid abundantly found in fruits and vegetables. It possesses a wide spectrum of biological activities, thus suggesting a role in disease prevention and health promotion. The present review aimed to uncover the bone-sparing effects of quercetin and its mechanism of action. Animal studies have found that the action of quercetin on bone is largely protective, with a small number of studies reporting negative outcomes. Quercetin was shown to inhibit RANKL-mediated osteoclastogenesis, osteoblast apoptosis, oxidative stress and inflammatory response while promoting osteogenesis, angiogenesis, antioxidant expression, adipocyte apoptosis and osteoclast apoptosis. The possible underlying mechanisms involved are regulation of Wnt, NF-κB, Nrf2, SMAD-dependent, and intrinsic and extrinsic apoptotic pathways. On the other hand, quercetin was shown to exert complex and competing actions on the MAPK signalling pathway to orchestrate bone metabolism, resulting in both stimulatory and inhibitory effects on bone in parallel. The overall interaction is believed to result in a positive effect on bone. Considering the important contributions of quercetin in regulating bone homeostasis, it may be considered an economical and promising agent for improving bone health. The documented preclinical findings await further validation from human clinical trials.

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Section: Type Of Animalmentioning

confidence: 91%

Section: In Vivo Evidence On the Effects Of Quercetin On Bonementioning

confidence: 99%

Section: The Underlying Mechanisms Of Action Of Quercetin As a Bonmentioning

confidence: 99%

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Quercetin as an Agent for Protecting the Bone: A Review of the Current Evidence

Wong

Chin

2020

IJMS

143

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“…A pilot study was performed to validate the chosen doses, which were also concurrent with the literature [ 18 , 19 ]. Animals in group 5 (positive drug control) were given 17β-estradiol (E2) dissolved in corn oil (25 mcg/kg/day, s.c.) [ 20 , 21 ] for a period of 12 weeks. Individual body weights were recorded at the end of the experiment.…”

Section: Methodsmentioning

confidence: 99%

Osteoprotective Activity and Metabolite Fingerprint via UPLC/MS and GC/MS of Lepidium sativum in Ovariectomized Rats

et al. 2020

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Lepidium sativum seeds are used traditionally to accelerate healing of bone fracture in addition to its culinary uses. This study aimed to characterize the osteoprotective effect of L. sativum in an ovariectomized rat model at two dose levels (50 and 100 mg/kg) using 17β-estradiol as a positive reference standard. Moreover, a complete metabolite profile of L. sativum via UHPLC/PDA/ESI–MS, as well as headspace solid-phase microextraction (SPME)-GC/MS is presented. Results revealed that L. sativum extract exhibited significant anti-osteoporotic actions as evidenced by mitigating the decrease in relative bone weight concurrent with improved longitudinal and perpendicular femur compression strength. Further, the extract enhanced the serum bone formation biomarkers lactate dehydrogenase (LDH) activity and osteocalcin levels. The extract also inhibited exhaustion of superoxide dismutase (SOD) as well as glutathione peroxidase (GPx) activities and accumulation of lipid peroxides in bone tissues. This is in addition to ameliorating the rise in the markers of bone resorption carboxyterminal telopeptide, type I (CTXI) and tartrate-resistant acid phosphatase (TRAP) and modulating receptor activator of nuclear factor kappa-Β ligand (RANKL)/ osteoprotegerin (OPG) expression. Metabolite characterization suggests that glucosinolates, lignans, coumarins, phenolic acids, and alkaloids mediate these anti-osteoporotic effects in a synergistic manner.

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“…By suppressing NF-κB activation, HIF-1α mRNA levels are reduced, bone histological characteristics are improved, and lumber strength is increased. 103 Apigenin, a flavonoid mainly existing in Apium graveolens L., can improve abnormal glucolipid metabolism by reducing the expression of HIF-1α and subsequent up-regulating PPARα-mediated CPT-1 and PDK-4 expressions and down-regulating PPARγ-mediated GPAT and GLUT-4 expressions. 104,105 In addition to flavonoids, quinones also have inhibitory effect on HIF-1α.…”

Section: Bioactive Componentsmentioning

confidence: 99%