Antioxidant activities and HepG2 cells growth inhibitory capacity of whole plant ethanol extracts (Eclipta alba Hassk and Mesona procumbens Hemsl)

Abstract

This study investigated in vitro antioxidant and hepatocellular carcinoma (HepG2) cells growth inhibitory effect of Eclipta alba Hassk (EH) and Mesona procumbens Hemsl (MH). Extracts from ethanol 50% (E50) were chosen for the assays. Total phenolic and flavonoid content and the antioxidant activities including ABTS 1 and DPPH radical-scavenging system were determined. The identification of phenolic compounds was analyzed by high performance liquid chromatography (HPLC). It was found that higher levels of DPPH and ABTS 1 radical-scavenging activity were detected from the EH extract with IC 50 of 41.91 6 0.08 and 75.49 6 1.15 mg/mL, respectively. The rate of DPPH radical-scavenging activity was significantly influenced by the reaction time. The 4hydroxybenzoic acid, caffeic acid, p-coumaric acid, and rosmarinic acid were the identified compounds in the EH. The 7-hydroxycoumarin, ferulic acid, and rutin being new compounds and two known compounds were identified in the MH. Lactate dehydrogenase released in medium was also evaluated. Extracts of the EH and MH stimulated the apoptotic factors bcl-2, bax, caspase-3, caspase-9 in HepG2. Overall, the EH extract was higher in total phenolic and flavonoid content, antioxidant potential and more effective in inhibiting cancer cell growth.

Functionality and Health-Promoting Properties of Polysaccharide and Plant-Derived Substances from Mesona chinensis

“…The results showed that the polyphenolic compounds found included protocatechuic acid, p-hydroxybenzoic acid, vanillic acid, caffeic acid, and syringic acid [18]. The ethanolic M. chinensis extraction yielded rosmarinic acid, apigenin, 7-hydroxycoumarin, ferulic acid, and rutin [16]. Eleven novel diterpenoids were reported as resulting from the methanolic extraction of M. chinensis after isolation with n-hexane and CH 2 Cl 2 , including seven ent-kauranes, three ent-atisanes, and one sarcopetalane [28].…”

“…However, drought in summer and cold winters lead to poor plant growth or even death, and impact the yield [14], with a reduced gross weight, number of roots, and aerial parts [15]. This herb is normally digested as a functional beverage and a semi-solid sweet dessert and has been used as a traditional Foods 2024, 13, 1134 2 of 16 medicine in China, Vietnam, and Indonesia for thousands of years [2,16,17]. The plant is used to treat heat shock, fever, hypertension, heart attack, diabetes, muscle pains, liver diseases, colon diseases, and inflammatory conditions, and also to alleviate muscle or joint pain [16,[18][19][20][21][22], because of the various active phytochemicals it contains, such as flavonoids and phenolic acid [23].…”

“…This herb is normally digested as a functional beverage and a semi-solid sweet dessert and has been used as a traditional Foods 2024, 13, 1134 2 of 16 medicine in China, Vietnam, and Indonesia for thousands of years [2,16,17]. The plant is used to treat heat shock, fever, hypertension, heart attack, diabetes, muscle pains, liver diseases, colon diseases, and inflammatory conditions, and also to alleviate muscle or joint pain [16,[18][19][20][21][22], because of the various active phytochemicals it contains, such as flavonoids and phenolic acid [23]. Mesona is also utilized to make a popular jelly dessert, due to its high polysaccharide component [24].…”

Screening and comparative study ofin vitroantioxidant and antimicrobial activities of ethanolic extracts of selected Vietnamese plants

“…Extracts of Blumea balsamifera (contained in the commercial product A), Cynara cardunculus var. scolymus, Phyllanthus emblica, Terminalia arjuna (contained in the commercial product B), or Phyllanthus urinaria and Eclipta prostrata (contained in the commercial product C) revealed different activities in the DPPH assay with IC 50 values of 72 µg/mL, [59] 23 µg/mL, [60] 11 µg/mL, [61] 8 µg/mL, [62] 17 µg/mL, [63] and 42 µg/ mL, [37] respectively. However, it is well known that many factors of manufacture (such as the materials, the extraction procedure of plant extracts, the ingredients, etc.)…”