Antioxidant activities of different fractions of polysaccharide purified from Gynostemma pentaphyllum Makino

Wang, Zhaojing; Luo, Dianhui

doi:10.1016/j.carbpol.2006.07.022

Cited by 159 publications

(82 citation statements)

References 22 publications

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“…thus, crude polysaccharides were mostly contained in the WE-Ep fraction, while in the WE-ES fraction, the polyphenol content was marked. plant polysaccharides and polyphenols are antioxidants (18), and they scavenge radicals by inhibiting initiation and breaking chain propagation by suppressing formation of free radicals by binding to metal ions, reducing hydrogen peroxide and quenching superoxide and singlet oxygen. in this study, the WE-Ep and WE-ES fractions exhibited SOd-like activity and dpph radical-scavenging activity, while the WE-ES fraction revealed higher antioxidant activity than WE-Ep.…”

Section: Discussionmentioning

confidence: 99%

Antioxidant and antitumor activities of water extracts from the root of Actinidia kolomikta

Guan

Zhang

Yang

et al. 2010

Experimental and Therapeutic Medicine

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Abstract. the genus of Actinidia is widely distributed throughout the Asian continent. Specific Actinidia species have been used as health foods and medical products for cancer treatment. Actinidia kolomikta is a species of wild plant that grows in the northern part of indochina. however, few studies on its bioactivity have been reported. in this study, the polysaccharide and polyphenol contents, the SOd-like activity and the dpph radical-scavenging activity of water extracts from the root of Actinidia kolomikta produced under different extraction temperatures were investigated. Furthermore, the water extraction-ethanol precipitate (WE-Ep) fraction and the water extraction-ethanol supernatant (WE-ES) fraction were used to test the anti-proliferative action on dld-1 colon cancer cells. Extracts produced using the 100˚C extraction procedure revealed higher extraction yields and antioxidant activities than extracts produced using the 40˚C extraction procedure. the WE-Ep and WE-ES fractions exhibited anti-proliferative effects on the dld-1 cells. moreover, the WE-ES polyphenolenriched fraction possessed more potent anti-proliferative effects on the dld-1 cells by inducing apoptosis compared to the WE-Ep polysaccharide fraction. medicinal plant extracts are generally considered to be relatively non-toxic at low doses and are not thought to cause major side effects compared to those observed with drugs. Wild A. kolomikta may provide an alternative to currently employed cancer therapies, and may be used as a natural health food with antioxidant actions.

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Section: Discussionmentioning

confidence: 99%

Antioxidant and antitumor activities of water extracts from the root of Actinidia kolomikta

Guan

Zhang

Yang

et al. 2010

Experimental and Therapeutic Medicine

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“…), Makino (PGP) were prepared using methods previously described with some modifications (Wang and Luo, 2007;Wang et al, 2012). Briefly, the dried GP were ground up carefully and turned into powder.…”

Section: Preparation Of Polysaccharides From Gynostemma Pentaphyllum mentioning

confidence: 99%

“…Qian et al (1999), report that PGP could markedly enhance the clearance of charcoal particles, increase the natural killer (NK) cells activity in liver cancer mouse model. Wang and Luo (2007), report that crude PGP exhibited scavenging capacities against hydroxyl radical and superoxide radicals. Chen et al (2011), demonstrated that the sulfated polysaccharide from GP have distinct antitumor activities.…”

Section: Introductionmentioning

confidence: 99%

Effects of Polysaccharides from <i>Gynostemma Pentaphyllum</i> (Thunb.), Makino on Physical Fatigue

Lin-Na¹,

Yong-Xiu²

2014

Afr. J. Trad. Compl. Alt. Med.

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Background: Gynostemma pentaphyllum (Thunb.) Makino has been reported to have a wide range of health benefits in Chinese herbal medicines. Polysaccharides from Gynostemma pentaphyllum (PGP), has been identified as one of the active ingredients responsible for its biological activities. Although many pharmacological activities of PGP have received a great deal of attention, there is limited evidence for the anti-fatigue effects of PGP. The purpose of this study was to investigate the effects of polysaccharides from PGP on physical fatigue. Materials and method: The rats were divided into four groups, with 10 animals per group: control (C), group, low-treated (LT), group, mediumtreated (MT), group, and high-treated (HT), group. The C group received distilled water, while LT, MT and HT groups were given various doses of PGP (100, 200, 400 mg/kg· d). After 30 days, forced swimming test was carried out in an acrylic plastic pool, then the exhaustive swimming time of rats and some biochemical parameters related to fatigue were measured. The data obtained showed that PGP could extend the exhaustive swimming time of the rats, as well as decrease the blood lactic acid (BLA), and blood urea nitrogen (BUN), concentrations, and increase the hemoglobin, liver glycogen and muscle glycogen concentrations. Result: The data obtained showed that different doses of PGP could extend the exhaustive swimming time of the rats, as well as decrease the BLA and BUN concentrations, and increase the hemoglobin, liver glycogen and muscle glycogen concentrations, which suggests that PGP had significant anti-fatigue effects on rats. Conclusion: PGP may be of use as a potential anti-fatigue agent, but there is a need for further research on long-term use in order to show its positive effects on physical fatigue.

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“…31,32 Superoxide anion radical, chemically generated in a beta-nicotinamide adenine dinucleotide-reduced (NADH)-phenazine methosulfate system, was detected by its ability to reduce nitroblue tetrazolium to a formazan dye. 32 The percent of superoxide anion radical scavenging activity was calculated as 100 · (C-S)/(C), where C is the absorbance of the control and S is the absorbance in the presence of the sample (extracts, quercetin).…”

Section: Superoxide Anion Radical Scavenging Assaymentioning

confidence: 99%

In VitroStudy on the Antioxidant Activity of a Polyphenol-Rich Extract fromPinus brutiaBark and Its Fractions

Creţu

Karonen

Salminen

et al. 2013

Journal of Medicinal Food

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A crude hydromethanolic extract from Pinus brutia bark and its fractions (diethyl ether, ethyl acetate, n-butanol, and aqueous fractions) were studied with regard to their phenolic content and antioxidant activities. The total phenolics and proanthocyanidins in each extract were quantified by spectrophotometric methods; the polyphenolic profile was analyzed by RP-HPLC-DAD-ESI-MS. All extracts were tested with regard to their ability to scavenge free radicals (ABTS radical cation, superoxide and hydroxyl radicals), reduce ferric ions, and inhibit 15-lipoxygenase. P. brutia bark extracts had high phenolic contents (303.79 -7.34-448.90 -1.39 mg/g). Except diethyl ether extract, all other extracts contained proanthocyanidins ranging from 225.79 -3.94 to 250.40 -1.44 mg/g. Several polyphenols were identified by RP-HPLC-DAD-ESI-MS: taxifolin in diethyl ether extract, a taxifolin-O-hexoside, catechin, procyanidin dimers, and trimers in ethyl acetate extract. Except diethyl ether extract, all other extracts were effective scavengers of superoxide and hydroxyl radicals (EC50 = 33.5 -1.1-54.93 -2.85 lg/mL and 0.47 -0.06-0.6 -0.0 mg/mL, respectively). All extracts had noticeable 15-lipoxygenase inhibitory effects (EC50 = 22.47 -0.75-34.43 -2.25 lg/mL). We conclude that P. brutia bark is very promising for the dietary supplements industry due to its high free radical scavenging and 15-lipoxygenase inhibitory effects.

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Antioxidant activities of different fractions of polysaccharide purified from Gynostemma pentaphyllum Makino

Cited by 159 publications

References 22 publications

Antioxidant and antitumor activities of water extracts from the root of Actinidia kolomikta

Antioxidant and antitumor activities of water extracts from the root of Actinidia kolomikta

Effects of Polysaccharides from <i>Gynostemma Pentaphyllum</i> (Thunb.), Makino on Physical Fatigue

In VitroStudy on the Antioxidant Activity of a Polyphenol-Rich Extract fromPinus brutiaBark and Its Fractions

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