Antioxidant Activity and Anticarcinogenic Effect of Extracts from Bouvardia ternifolia (Cav.) Schltdl.

Abstract

According to the available ethnobotanical data, the Bouvardia ternifolia plant has long been used in Mexican traditional medicine to relieve the symptoms of inflammation. In the present study, the cytotoxic effect of extracts obtained from the flowers, leaves and stems of B. ternifolia using hexane, ethyl acetate (AcOEt) and methanol (MeOH) was evaluated by applying them to the SiHa and MDA-MB-231 cancer cell lines. An MTT reduction assay was carried out along with = biological activity assessments, and the content of total phenols, tannins, anthocyanins, betalains and saponins was quantified. According to the obtained results, nine extracts exhibited a cytotoxic effect against both the SiHa and MDA lines. The highest cytotoxicity was measured for leaves treated with the AcOEt (ID50 of 75 µg/mL was obtained for MDA and 58.75 µg/mL for SiHa) as well as inhibition on ABTS•+ against DPPH• radical, while MeOH treatment of stems and AcOEt of flowers yielded the most significant antioxidant capacity (90.29% and 90.11% ABTS•+ radical trapping). Moreover, the highest phenolic compound content was measured in the stems (134.971 ± 0.294 mg EAG/g), while tannins were more abundant in the leaves (257.646 mg eq cat/g) and saponins were most prevalent in the flowers (20 ± 0 HU/mg). Screening tests indicated the presence of flavonoids, steroids, terpenes and coumarins, as well as ursolic acid, in all the studied extracts. These results demonstrate the biological potential of B. ternifolia.

Αnti-prion effects of anthocyanins

“…Oenin (0911S, Extrasynthese Genay, France) and Myrtillin (0938S, Extrasynthese Genay, France) were dissolved in dimethyl sulfoxide (DMSO). Lethal Dose 50% (LD 50 ) values were estimated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) Assay (M6494, Invitrogen, Waltham, MA, USA), following 48-h incubation [ 65 , 66 ] with gradually increased concentrations of Oenin and Myrtillin. Control cells were treated with DMSO in concentrations matching those delivered with the compounds.…”

Anti-Prion Effects of Anthocyanins

“…Cells were cultured in Opti-MEM (51985042, Invitrogen Waltham, MA, USA) supplemented with 10% Fetal Bovine Serum (FBS) under a 5% CO 2 at 37 • C. Oenin (0911S, Extrasynthese Genay, France) and Myrtillin (0938S, Extrasynthese Genay, France) were dissolved in dimethyl sulfoxide (DMSO). Lethal Dose 50% (LD 50 ) values were estimated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) Assay (M6494, Invitrogen, Waltham, MA, USA), following 48-h incubation [65,66] with gradually increased concentrations of Oenin and Myrtillin. Control cells were treated with DMSO in concentrations matching those delivered with the compounds.…”