Antioxidant activity and Cytotoxicity Effect of Various Extracts of Sclerorhachis platyrachis on the Human breast adenocarcinoma Cells

Abstract

Background and Aim: One of the spices of compositae is the Sclerorhachis platyrachis that is Iranian native. This aromatic plant has been known as bacterial growth inhibitor. This study have been attempted to investigate antioxidant activity and cytotoxicity effect of Sclerorhachis platyrachis extractson the MCF-7 cell line. Materials and Methods:The Hexane (HE), Chloroform (CE), Ethyl acetate (EE), Methanolic extracts (ME) of Sclerorhachis platyrachis leaves prepared by usingmaceration method. The DPPH assay was used for determination of antioxidant activity. Also, total phenolic content measured. The cytotoxicity of the extracts on MCF-7 and HEK-293 cells evaluated using MTT assay in both 48h and 72h. Results: evaluation of antioxidant activity indicated that radical scavenging ability was as ascorbic acid> ME > EE > CE > HE, While, total phenolic content was as CE>ME>EE>HE. The highest and the lowest cytotoxicity against MCF-7 cells were related to CE and ME respectively. Except for ethyl acetate extract, all extract were non toxic on the HEK-293 cells. Conclusion: Our findings showed that the chloroform extract of aerial part of Sclerorhachis platyrachis had the strongest effect against breast cancer carcinoma (MCF-7). Whereas, the extracts of this plant had no cytotoxicity on the normal cells.