Antioxidant Activity and Inhibitory Effect of Some Commonly used Medicinal Plants against Lipid Per-Oxidation in Mice Brain

Abstract

Background: The present study compares the protective properties of aqueous extracts of six medicinal plants, Phyllanthus emblica, Terminalia chebula (black and yellow), Terminalia arjuna, Balsamodendron Mukul and Alium sativum against lipid per-oxidation in mice brain. Methods:The antioxidant activities were analyzed by lipid per-oxidation assay, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical assay, total antioxidant activity and metal chelation. Results: The extracts (fruits and bark) showed inhibition against thiobarbituric acid reactive species (TBARS) induced by pro-oxidant (10 µM FeSO4) in mice brain. Moreover, the free radical scavenging activities of the extracts was evaluated by the scavenging of DPPH radical (IC 50 , 23.23±1.2 µg/ml (Phyllanthus emblica), 20.24±0.9 µg/ml (Terminalia chebula yellow) and 17.33±1.1 µg/ml (Terminalia chebula black), 19.44±0.45 µg/ml (Terminalia arjuna), 56.59±2.1 µg/ml (Balsamodendron Mukul) and ˂ 200 µg/ml (Alium sativum). Conclusion: The higher antioxidant and inhibitory effect of Terminalia chebula black in this study could be attributed to its significantly higher phenolic content, Fe(II) chelating ability, reducing ability and free radical scavenging activity. Therefore oxidative stress in brain could be potentially prevented by the intake of these plants.

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“…There are many chemical compounds in alcuremon, and the main biologically active components of these plants are polyphenols, terpenes, anthocyanins, flavonoids, alkaloids, tannin, glycosides etc. 19,20,21,22,23,24,25,26 .…”

“…Alcuremon contains extract of Gentiana barbata Froel., Carthamus tinctorius L., Terminalia chebula Retz, and Rosa acicularis Lindl., these are composed of many chemical compounds, including xanthones, polyphenols, flavonoids, secoiridoids, rutin, tannins, antioxidant and others. These biologically active compounds had a wide range of pharmacological activities including antioxidant, anticancerogenic, antimutagenic, hepatoprotective, cytoprotective, cardioprotective, neuroprotective, antiviral, antiinflammatory and wound healing [19][20][21][22][23][24][25][26] . D Diosmetin is a flavonoid of the alcuremon and, it barely activates TrkB, like brain-derived neurotrophic factor (BDNF), also suppresses caspase-3 activation in neurons weakly and nonselective 27 .…”

In-vivo Study on Organometallic Compounds as Anticancer Agents

“…Likewise the interaction of one metal with another can also be explored taking synergestic effect into consideration. Not only this, whether the complex acts in unison or metal and ligand act independently inside the body needs a greater depth of understanding by bridging the gap between chemistry and molecular biology [7].…”

In vitro evaluation of free radical-scavenging potentials of ethanol extract of combretum dolichopentalum leaves

“…Nevertheless, EECD prevented peroxidation in rabbit brain homogenate at 64.70 μg/ml (IC 50 ). The brain is particularly vulnerable to oxidative damage because of its high oxygen utilization, high content of oxidizable polyunsaturated fatty acids and the presence of redox-active metals (Cu, Fe) [28][29][30]. Polyphenols cross the blood-brain barrier and can exert its antioxidant and iron chelating properties in the brain [23].…”