Antioxidant Activity, Functional Properties, and Cytoprotective Effects on HepG2 Cells of Tree Peony (Paeonia suffruticosa Andr.) Seed Protein Hydrolysate as Influenced by Molecular Weights Fractionation

Wang, Yingying; Li, Yingqiu; Wang, Chenying; He, Juan; Mo, Haizhen

doi:10.3390/foods11172592

Cited by 13 publications

(6 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, the size of the peptide has an effect on its antioxidant activity; the low molecular weight peptide (less than 10 kDa) easily interacts with free radicals and is good for adsorption in cells [41,42]. showed no toxicity toward both cell lines at 0.98-62.50 µg/mL, however at higher concentration (125-500 µg/mL), it showed slight toxicity that was significantly different from that of the control group.…”

Section: Resultsmentioning

confidence: 99%

“…WB-G protein hydrolysate was shown to have high antioxidant activity and can donate electrons to free radicals or ROS to decrease the damage of H2O2 [49]. In addition, the molecular weight of protein hydrolysate from WB-G was smaller and could more easily interact with free radicals and halt the chain Functional Foods in Health and Disease 2023; 13(4): 208-224 FFHD Page 218 of 224reaction to prevent damage to essential chemicals[50].Watermelon seed protein hydrolysate with low molecular weight proteins or peptides (5-10 kDa)showed a cytoprotective effect against H2O2 in the HepG2 cell line[42]. Soybean protein hydrolysate protected Caco-2 cells against H2O2-induced oxidative damage by downregulating ROS in the cell[51].…”

mentioning

confidence: 99%

See 1 more Smart Citation

Antioxidant potential of gamma ray irradiated winged bean (Psophocarpus tetragonolobus) seed protein hydrolysate

Hlosrichok¹,

Aunpad²

2023

FFHD

View full text Add to dashboard Cite

Background: Winged bean or Psophocarpus tetragonolobus (WB) seeds have high protein content and could be applied as a source of antioxidant proteins and peptides. The utilization of gamma rays in plant protein extraction provides consumers with a safe and harmless technology.Objective: To determine the efficiency of gamma rays in the protein extraction process of WB seeds and investigate the antioxidant activity, cytotoxicity, cytoprotective effect of the gamma ray irradiated WB (WB-G) seed protein hydrolysate.Methods: WB seeds were irradiated with gamma ray (WB-G) and total protein were extracted by acid-base extraction method. The total protein was further hydrolyzed with alcalase enzyme to obtain the protein hydrolysate. The antioxidant activity was evaluated by DPPH and ABTS assay. The cytotoxicity and cytoprotective effect were determined by MTT assay. The cellular reactive oxygen species was further analyzed by ACFDA assay.Results: The protein extracted from WB-G seeds showed higher protein yield and antioxidant activity than that of non-irradiated seeds. After hydrolysis with alcalase enzyme at 55°C for 6 hours, WB-G protein hydrolysate with degree of hydrolysis of 90.00±0.91% exhibited strong antioxidant activity with IC50 value of 10.30±0.02 µg/ml and 3.10±0.06 µg/ml as measured by DPPH and ABTS assay, respectively. Moreover, it showed no toxicity toward L929 mouse fibroblast cells and IPEC-J2 cell lines and can reduce the damage caused by H2O2. As determined in cellular reactive oxygen species by H2-DCFDA assay, the WB-G protein hydrolysate can reduce the level of ROS and subsequently the damage of cell by H2O2. Conclusions: This study demonstrated that gamma ray irradiation on the WB seeds can increase the protein yield and its protein hydrolysate exhibited promising antioxidant activity and cytoprotective effect on cells against H2O2 damage. It might be developed as an alternative food or feed supplement.Keywords:Psophocarpus tetragonolobus; Gamma rays; Protein hydrolysate; Antioxidant activity; Cytoprotective effect

show abstract

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Antioxidant potential of gamma ray irradiated winged bean (Psophocarpus tetragonolobus) seed protein hydrolysate

Hlosrichok¹,

Aunpad²

2023

FFHD

View full text Add to dashboard Cite

show abstract

“…These cells are sensitive to cytotoxic effects and are therefore widely used in test systems in original studies of promising pharmacological drugs [ 48 ]. When studying the systemic metabolism of drugs, cell lines of hepatocyte origin are used, such as the HepG2 cell line [ 49 , 50 , 51 ], which we used to determine the possible risks of the applied combination of ascorbic acid and lysozyme hydrochloride on SH-groups, TBA-active components, and catalase activity as important indicators of antioxidant protection. MAEC line was used as a model system that plays an important role in the endothelial barrier of drug permeability [ 52 , 53 ] to determine possible changes in the morphofunctional features of these cells under the action of ascorbic acid and lysozyme hydrochloride.…”

Section: Resultsmentioning

confidence: 99%

The Study of the Cytotoxicity, Proliferative and Microbiological Activity of the Medicated Chewing Gum with Ascorbic Acid and Lysozyme Hydrochloride Using Different Culture of Cells

et al. 2023

View full text Add to dashboard Cite

Medicated chewing gum with lysozyme hydrochloride and ascorbic acid as active pharmaceutical ingredients was developed for application in dentistry. The aim of this research was to study the cytotoxicity, proliferative, and microbiological activities of the active ingredients in different types of cell cultures. The preclinical study of active pharmaceutical ingredients and their combinations was carried out using culture lines such as HepG2 (human hepatocarcinoma cells), Hek293 (human embryonic kidney cells), and MAEC (mouse aortic endothelial cells). MTT assays were used to analyse cytotoxicity and proliferative activity, while the state of antioxidant protection was assessed by the content of sulfhydryl groups and catalase activity. The determination of lipid peroxidation products was based on the level of TBA-active products. As a microbiological model for studying the effect of the developed dental medicine on the ability of the oral cavity microorganisms to form biofilms, the following strains were used: Streptococcus mutans, Staphylococcus aureus, Staphylococcus epidermidis, Lactobacillus plantarum, and Candida albicans. The optical density of the formed biofilm was evaluated by the intensity of the experimental sample’s colour on a StatFax 303 Plus photometer at a wavelength of 630 nm. The combination of ascorbic acid and lysozyme hydrochloride in the established concentrations (20 mg and 10 mg per 1 gum, respectively) resulted in a slight stimulation of cell proliferation without any toxic effects and increased antioxidant protection, preventing the development of oxidative stress. It was found that, in contrast to the separately used active substances, the combination of lysozyme hydrochloride and ascorbic acid inhibits the biofilm formation of all studied microorganisms and shows the ability to destroy diurnal biofilms of L. plantarum and fungi of the genus Candida, indicating potentiation and summation of the active pharmaceutical ingredients’ composition effects in the developed dental medicine. Due to the observed positive pharmacological and microbiological action, the combination of lysozyme hydrochloride and ascorbic acid in the medicated chewing gum serves as a promising tool for the prevention and treatment of infectious and inflammatory diseases of the periodontium and mucous membranes and the prevention of caries.

show abstract

“…This indicated that the molecular weights of prolamins from highland barley are significantly larger than those in zein. Almost all physico-chemical properties of proteins, including viscosity, emulsifying property, gel property, and filming-forming properties, etc., are closely related to the molecular weight [ 15 , 16 ]. This indicates that prolamins from highland barley may exhibit quite different functional properties from zein.…”

Section: Resultsmentioning

confidence: 99%

The Composition, Structure, and Functionalities of Prolamins from Highland Barley

Xing

Zhang

et al. 2023

Molecules

View full text Add to dashboard Cite

The composition, structure, and functionalities of prolamins from highland barley were investigated. These parameters were compared with those of the commonly applied prolamins (zein). There are more charged and hydrophilic amino acids in highland barely prolamins than zein. The molecular weight of highland barely prolamins was between 30 and 63 kDa, which was larger than that of zein (20 and 24 kDa). The main secondary structure of highland barely prolamins was β-turn helices, while α-helical structures were the main secondary structure in zein. The water holding capacity, thermal stability, emulsifying capacity, and stability of prolamins from highland barley were significantly higher than in zein, while the opposite results were observed for oil absorption capacity between the two. The diameter of fibers prepared using highland barely prolamins was almost six times that of zein, while highland barely prolamins formed ribbon structures instead of fibers. Therefore, the results provide guidance for applications of prolamins from highland barley.

show abstract

Antioxidant Activity, Functional Properties, and Cytoprotective Effects on HepG2 Cells of Tree Peony (Paeonia suffruticosa Andr.) Seed Protein Hydrolysate as Influenced by Molecular Weights Fractionation

Cited by 13 publications

References 35 publications

Antioxidant potential of gamma ray irradiated winged bean (Psophocarpus tetragonolobus) seed protein hydrolysate

Antioxidant potential of gamma ray irradiated winged bean (Psophocarpus tetragonolobus) seed protein hydrolysate

The Study of the Cytotoxicity, Proliferative and Microbiological Activity of the Medicated Chewing Gum with Ascorbic Acid and Lysozyme Hydrochloride Using Different Culture of Cells

The Composition, Structure, and Functionalities of Prolamins from Highland Barley

Contact Info

Product

Resources

About