Antioxidant additive melatonin in tris-based egg yolk extender improves post-thaw sperm attributes in Hariana bull

YADAV, DILEEP KUMAR; Kumar, Anuj; Gupta, Shashikant; Sharma, Pratishtha; Kumar, Gyanesh; Sachan, Vikas; Yadav, Brijesh; Yadav, Sarita; Saxena, Atul; Swain, Dilip Kumar

doi:10.1016/j.anireprosci.2023.107214

Cited by 2 publications

(2 citation statements)

References 69 publications

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“…The performance and emission behavior of methyl ester Mangifera indica oil (MEMSO), used in diesel engines, were studied by Yadav. 6 All of the mixes had lower BTEs and smoke and HC emissions than diesel under all load circumstances. Additionally, because of their reduced calorific value, MEMSO blends' BSFC is greater than diesel's.…”

Section: Biodieselmentioning

confidence: 99%

Enhancing Performance and Emission Characteristics of Biodiesel-Operated Compression Ignition Engines through Low Heat Rejection Mode and Antioxidant Additives: A Review

Rajendran,

Venkatesan,

Dhairiyasamy

et al. 2023

ACS Omega

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Depending on the heat content and compression ignition (CI) engine combustion, biodiesel is a viable substitute fuel. Biodiesel is an oxygenated, safe, sulfur-free, biodegradable, and renewable fuel. It may be utilized in CI engines in any combination with diesel fuel without requiring the engine to be significantly modified. Many research studies have been made with several biodiesels as diesel substitutes, including Pongamia pinnata, Jatropha curcas, Mangifera indica, and Madhuca longifolia. The topic of the current review is the potential of renewable fuels to outperform diesel fuel in terms of performance, combustion, and emission characteristics. In the present study, CI engines are fueled with biodiesels made from Man. indica, Mad. longifolia, and pongamia seed oil. Adopting low heat rejection (LHR) mode CI engines and adding an antioxidant agent in addition to the biodiesel blends may resolve the issue of these biodiesels’ poorer performance and increased NO emission. Both these additions may provide positive approaches in both performance and emission.

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Section: Biodieselmentioning

confidence: 99%

Enhancing Performance and Emission Characteristics of Biodiesel-Operated Compression Ignition Engines through Low Heat Rejection Mode and Antioxidant Additives: A Review

Rajendran,

Venkatesan,

Dhairiyasamy

et al. 2023

ACS Omega

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“…Decreasing the ROS damage by reducing ROS levels is essential to maintain sperm quality during preservation in vitro. Indeed, studies have shown that antioxidants such as ascorbic acid [11], lycopene [12], melatonin [13], resveratrol [14], vitamin E [15], and epimedium [16] have been added to sperm diluents to improve ram sperm quality during storage at 4 • C or cryopreservation. In addition, Buck et al (2012) [17] found that the addition of 2 and 4 mM methionine, one of the precursors of glutathione, improved ram sperm viability and mitochondrial activity during the 96 h of storage at 5 • C, when compared to the control.…”

Section: Introductionmentioning

confidence: 99%

Pyrroloquinoline Quinone Improves Ram Sperm Quality through Its Antioxidative Ability during Storage at 4 °C

Zhu,

Li,

Yang

et al. 2024

Antioxidants

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Sperm motility is an important factor in the migration of sperm from the uterus to the oviduct. During sperm preservation in vitro, sperm generates excessive ROS that damages its function. This study aims to investigate whether the addition of pyrroloquinoline quinone (PQQ) to the diluted medium could improve chilled ram sperm quality, and then elucidates the mechanism. Ram semen was diluted with Tris-citric acid-glucose (TCG) medium containing different doses of PQQ (0 nM, 10 nM, 100 nM, 1000 nM, 10,000 nM), and stored at 4 °C. Sperm motility patterns, plasma membrane integrity, acrosome integrity, mitochondrial membrane potential, reactive oxygen species (ROS) levels, malondialdehyde (MDA) levels, superoxide dismutase (SOD) activity, and ATP levels were measured after preservation. Furthermore, the expressions of NADH dehydrogenase 1 (MT-ND1) and NADH dehydrogenase 6 (MT-ND6) in sperm were also detected by western blotting. In addition, sperm capacitation and the ability of sperm to bind to the zona pellucina were also evaluated. It was observed that the addition of PQQ significantly (p < 0.05) improved ram sperm motility, membrane integrity, and acrosome integrity during preservation. The percentage of sperm with high mitochondrial membrane potential in the PQQ treatment group was much higher than that in the control. In addition, supplementation of PQQ also decreased the sperm MDA and ROS levels, while increasing ATP levels. Interestingly, the levels of MT-ND1 and MT-ND6 protein in sperm treated with PQQ were also higher than that of the control. Furthermore, the addition of 100 nM PQQ to the medium decreased ROS damage in MT-ND1 and MT-ND6 proteins. The addition of 100 nM PQQ significantly (p < 0.05) increased protein tyrosine phosphorylation in ram sperm after induced capacitation. Furthermore, the value of the sperm–zona pellucida binding capacity in the 100 nM PQQ treatment group was also much higher than that of the control. Overall, during chilled ram- sperm preservation, PQQ protected ram sperm quality by quenching the ROS levels to reduce ROS damage and maintain sperm mitochondrial function, and preserved the sperm’s high ability of fertilization.

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Antioxidant additive melatonin in tris-based egg yolk extender improves post-thaw sperm attributes in Hariana bull

Cited by 2 publications

References 69 publications

Enhancing Performance and Emission Characteristics of Biodiesel-Operated Compression Ignition Engines through Low Heat Rejection Mode and Antioxidant Additives: A Review

Enhancing Performance and Emission Characteristics of Biodiesel-Operated Compression Ignition Engines through Low Heat Rejection Mode and Antioxidant Additives: A Review

Pyrroloquinoline Quinone Improves Ram Sperm Quality through Its Antioxidative Ability during Storage at 4 °C

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