Antioxidant and anticoagulant activity of sulfated polysaccharide from Gracilaria debilis (Forsskal)

Sudharsan, S.; Subhapradha, Namasivayam; Seedevi, Palaniappan; Shanmugam, Vairamani; Madeswaran, Perumal; Shanmugam, Annaian; Srinivasan, Alagiri

doi:10.1016/j.ijbiomac.2015.09.046

Cited by 75 publications

(38 citation statements)

References 44 publications

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“…The peak of 3409–3432 cm −1 was caused by the stretching vibration of O-H, and 2920–2928 cm −1 was caused by the stretching vibration of C-H; the peak near 1380 cm −1 was caused by the deforming vibration of C-H bond. The peak at about 1625 cm −1 was attributed to the asymmetric and symmetrical stretching vibration of −COOH [ 24 , 25 ]. The peak at 1370 cm −1 was the signal area of ester sulfate [ 26 ], and the peak near 1260 cm −1 corresponds to S=O vibration of the sulfate groups [ 27 ].…”

Section: Resultsmentioning

confidence: 99%

“…Sparassis crispa polysaccharide fraction with a molecular weight of 75 kDa reduced the accumulation of reactive oxygen species, blocked Ca 2+ influx, and prevented depolarization of the mitochondrial membrane potential to protect PC12 cells against L-Glu-induced injury [ 45 ]. GLP can repair the mitochondria in damaged cells because −OSO 3 H and −COOH functional groups are rich in GLP, which can scavenge reactive oxygen species [ 24 ].…”

Section: Discussionmentioning

confidence: 99%

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Structural Characterization and Repair Mechanism of Gracilaria lemaneiformis Sulfated Polysaccharides of Different Molecular Weights on Damaged Renal Epithelial Cells

Guo

Sun

et al. 2018

Oxidative Medicine and Cellular Longevity

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Natural Gracilaria lemaneiformis sulfated polysaccharide (GLP0, molecular weight = 622 kDa) was degraded by H2O2 to obtain seven degraded fragments, namely, GLP1, GLP2, GLP3, GLP4, GLP5, GLP6, and GLP7, with molecular weights of 106, 49.6, 10.5, 6.14, 5.06, 3.71, and 2.42 kDa, respectively. FT-IR and NMR results indicated that H2O2 degradation does not change the structure of GLP polysaccharides, whereas the content of the characteristic −OSO3H group (13.46% ± 0.10%) slightly increased than that of the natural polysaccharide (13.07%) after degradation. The repair effects of the polysaccharide fractions on oxalate-induced damaged human kidney proximal tubular epithelial cells (HK-2) were compared. When 60 μg/mL of each polysaccharide was used to repair the damaged HK-2 cells, cell viability increased and the cell morphology was restored, as determined by HE staining. The amount of lactate dehydrogenase released decreased from 16.64% in the injured group to 7.55%–13.87% in the repair groups. The SOD activity increased, and the amount of MDA released decreased. Moreover, the mitochondrial membrane potential evidently increased. All polysaccharide fractions inhibited S phase arrest through the decreased percentage of cells in the S phase and the increased percentage of cells in the G2/M phase. These results reveal that all GLP fractions exhibited repair effect on oxalate-induced damaged HK-2 cells. The repair ability is closely correlated with the molecular weight of the fractions. GLP2 with molecular weight of about 49.6 kDa exhibited the strongest repair effect, and GLP with higher or lower molecular weight than 49.6 kDa showed decreased repair ability. Our results can provide references for inhibiting the formation of kidney stones and developing original anti-stone polysaccharide drugs.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Structural Characterization and Repair Mechanism of Gracilaria lemaneiformis Sulfated Polysaccharides of Different Molecular Weights on Damaged Renal Epithelial Cells

Guo

Sun

et al. 2018

Oxidative Medicine and Cellular Longevity

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“…Pereira et al [ 150 ] found that the sulfated galactans from the red alga Botryocladia occidentalis have higher anticoagulant properties than similar polysaccharides isolated from Gelidium crinale with different sulfation patterns. Sudharsan et al [ 151 ] showed that the sulfated polysaccharide isolated from red marine seaweed Gracilaria debilis presented an important anticoagulant ability through APTT and PT (14.11 and 8.23 IU/mg) assays. Silva et al [ 145 ] discussed the anticoagulant properties of sulfated galactans from seaweeds and showed their close dependence on the monosaccharidic composition, the type of glycosidic linkages, the sulfate content, the molar mass and the sulfation position in the backbone structure.…”

Section: Health Claims Of Algal Polysaccharidesmentioning

confidence: 99%

Bioactive Polysaccharides from Seaweeds

et al. 2020

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Bioactive compounds with diverse chemical structures play a significant role in disease prevention and maintenance of physiological functions. Due to the increase in industrial demand for new biosourced molecules, several types of biomasses are being exploited for the identification of bioactive metabolites and techno-functional biomolecules that are suitable for the subsequent uses in cosmetic, food and pharmaceutical fields. Among the various biomasses available, macroalgae are gaining popularity because of their potential nutraceutical and health benefits. Such health effects are delivered by specific diterpenes, pigments (fucoxanthin, phycocyanin, and carotenoids), bioactive peptides and polysaccharides. Abundant and recent studies have identified valuable biological activities of native algae polysaccharides, but also of their derivatives, including oligosaccharides and (bio)chemically modified polysaccharides. However, only a few of them can be industrially developed and open up new markets of active molecules, extracts or ingredients. In this respect, the health and nutraceutical claims associated with marine algal bioactive polysaccharides are summarized and comprehensively discussed in this review.

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“…The optical rotation of AGP33 was found to be rαs An earlier study has shown that polysaccharides from abalone gonads were sulfated polymers accounting for one-tenth of the total gonad mass [2]. It is widely recognized that most of the sulfated polysaccharides can serve as anticoagulants [3][4][5][6]. For example, the potent anticoagulant heparin can activate antithrombin III, which blocks thrombin from clotting the blood stream.…”

Section: Preparation and Characterization Of Abalone Gonad Polysacchamentioning

confidence: 99%

“…It is widely recognized that most of the sulfated polysaccharides can serve as anticoagulants [3][4][5][6]. For example, the potent anticoagulant heparin can activate antithrombin III, which blocks thrombin from clotting the blood stream.…”

Section: Introductionmentioning

confidence: 99%

Anticoagulant Activity and Structural Characterization of Polysaccharide from Abalone (Haliotis discus hannai Ino) Gonad

et al. 2016

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Abstract:In this study, we aimed at characterizing the structure and the anticoagulant activity of a polysaccharide fraction (AGP33) isolated from the gonads of Haliotis discus hannai Ino. AGP33 was extracted by enzymatic hydrolysis and purified by ion-exchange and gel-filtration chromatography. The backbone fraction of AGP33 (BAGP33), which appeared to contain of mannose, glucose and galactose, was prepared by partial acid hydrolysis. According to methylation and nuclear magnetic resonance (NMR) spectroscopy, the backbone of AGP33 was identified as mainly consisting of 1Ñ3-linked, 1Ñ4-linked, and 1Ñ6-linked monosaccharides. AGP33 is a sulfated polysaccharide with sulfates occur at 3-O-and 4-O-positions. It prolonged thromboplastin time (APTT), thrombin time (TT) and prothrombin time (PT) compared to a saline control solution in a dosage-dependent manner. AGP33 exhibited an extension (p < 0.01) of APTT compared to the saline group at concentrations higher than 5 µg/mL. AGP33 exhibited higher anticoagulant activity than its desulfated product (AGP33-des) and BAGP33. The results showed that polysaccharide with higher molecular weight and sulfate content demonstrated greater anticoagulant activity.

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Antioxidant and anticoagulant activity of sulfated polysaccharide from Gracilaria debilis (Forsskal)

Cited by 75 publications

References 44 publications

Structural Characterization and Repair Mechanism of Gracilaria lemaneiformis Sulfated Polysaccharides of Different Molecular Weights on Damaged Renal Epithelial Cells

Structural Characterization and Repair Mechanism of Gracilaria lemaneiformis Sulfated Polysaccharides of Different Molecular Weights on Damaged Renal Epithelial Cells

Bioactive Polysaccharides from Seaweeds

Anticoagulant Activity and Structural Characterization of Polysaccharide from Abalone (Haliotis discus hannai Ino) Gonad

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