Antioxidant and metal chelating activities of Lagenaria siceraria (Molina) Standl peel, pulp and aerial parts in relation to their total phenol and flavonoid content

Abstract

Objective: The aim of the present study was to evaluate the antioxidant activity and total phenol and flavonoid content of different parts (peel, pulp and aerial parts) of Lagenaria siceraria. Method: Successive extraction was done by cold percolation method using solvents of different polarity viz. petroleum ether, toluene, ethyl acetate, acetone, water. Total phenol content was determined by Folin-Ciocalteu's reagent method and flavonoid was determined by aluminium chloride colorimetric method. The antioxidant assays evaluated were 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity, 2, 2'-Azino-bis-(3-ethyl) benzothiazoline-6-sulfonic acid (ABTS) radical cation scavenging activity and Ferric reducing antioxidant power. Results: L. siceraria peel possessed maximum phenol and flavonoid content in acetone and toluene extracts respectively. FRAP was maximum in acetone extract of peel. The peel extract demonstrated stronger DPPH activity with IC 50 value of 111 µg/ml followed by pulp extract. The same extract was effective in scavenging ABTS radical with an IC 50 value of 39 µg/ml while other parts were ineffective. Conclusion: This work demonstrated good antioxidant activity of L. siceraria vegetable cultivated in India and recommends that the peel of this vegetable may be of interest from a functional point of view as a major source of natural antioxidant.

Optimizing the Integration of Microwave Processing and Enzymatic Extraction to Produce Polyphenol-Rich Extracts from Olive Pomace

“…The aqueous ABTS + solution was diluted with water to obtain an absorbance of 0.7 at 734 nm. Subsequently, 3 mL of ABTS + solution was added to 30 µL of the extracts [25]. After 6 min, absorbance was measured at 734 nm using a Thermo Genesys 10S spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA).…”

In vitro screening of elastase, collagenase, hyaluronidase, and tyrosinase inhibitory and antioxidant activities of 22 halophyte plant extracts for novel cosmeceuticals

“…In addition, the exalted levels of ROS can cause not only the senescence and damage of biological functions such as lipid membrane peroxidation, DNA deterioration, cell death but also human's disease, namely, cancer, stroke, Parkinson's disease, heart disease, arteriosclerosis, infection, and autoimmune disease (Popoola et al 2015). It has been carried out that an antioxidant provided a great potential to defend ROS and deplete the oxidative stress, thus, a compound with the strong antioxidant activity additionally facilitates the skin protecting against the oxidative damages along with delaying the skin aging process (Chanda et al 2015;Palmer and Kitchin 2010).…”