Antioxidant and Total Phenolic Content of Catharanthus roseus Using Deep Eutectic Solvent

Abstract

Deep eutectic solvents as a new type of eco-friendly solvents have attracted attention in chemistry, medicine, and other fields for the extraction and separation of target compounds from medicinal plants. Deep eutectic solvents are easy to prepare and have many advantages as solvents, such as chemical inertness with water, low cost, easy biodegradability, and pharmaceutically acceptable toxicity. In this study, a deep eutectic solvent made up of choline chloride-glycerol (1:2) was used for the extraction of flavonoids from Catharanthus roseus plant parts (flower petal, leaves, stem, and root). The highest amount of phenolic content was detected in flower petal, that is, 194.50 mg GAE/g. In DPPH test, the maximum amount of antioxidant activity determined in the flower petal was 73.13%; IC 50 was calculated by using a linear regression equation; IC 50 value of the standard, stem, root, leaf, and flower petal was 13.22, 90.44, 83.93, 120.14, 79.49 μg/ml, respectively. The result of this research is that Catharanthus roseus has a compatible antioxidant activity. This can be helpful for the treatment of diseases caused by free-radical oxidative stress.

Determination of total phenolic, flavonoids and antioxidant activity in Calligonum polygonoides L. from Thar Desert

“…The results of phenolic content of clove (highest phenolic containing plant food reported till date) in our study were completely resembling with previous reports (Perez-Jimenez et al, 2010). The phenolic content of bark and root tissues of C. polygonoides is higher than that of some medicinal plants like red clover, Catharanthus roseus and Marrubium peregrinum, which posses abou 4688, 18280 and 8978 mg GAE -1 100 g phenolics (Nisar et al, 2017). The flavonoid content was higher in roots and bark while lower in flower bud and foliage than -1 red clover (2661 mg 100 g ) (Esmaeili et al, 2015).…”

Total phenolic content (TPC) in Catharanthus roseus and Clitoria ternatea leaves extract

“…The negative control for the research is ethanol. The step used by Nisar et al (2017), with slight modification where 100mg of gallic acid was dissolved in 100 ml ethanol and then diluted to 20, 40, 60, 80, and 100 mg/ml was used. The curve was obtained by measuring the absorbance of gallic acid at 765nm with a UV spectrophotometer.…”

Ecofriendly ruthenium-containing nanomaterials: synthesis, characterization, electrochemistry, bioactivity and catalysis

“…21 Various plants, particularly Catharanthus roseus , Nephrolepis biserrata , Cycas revoluta , Ocimum tenuiflorum , Diospyros kaki , Gloriosa superba , Aspalathus linearis , Cacumen platycladi , and Dictyota dichotoma , are known for their significant reducing and antioxidant properties. 22 High-temperature treatment and bio extracts cause reduction of Ru 3+ , followed by its facile stabilization via bioactive compounds. However, the oxidation of Ru +3 to Ru +4 (s 0 d 5 to s 0 d 4 ) to synthesize RuO 2 NPs is found to be more facile than the synthesis of Ru and Ag NPs.…”