Antioxidant and Understanding the Anticancer Properties in Human Prostate and Breast Cancer Cell Lines of Chemically Characterized Methanol Extract from Berberis hispanica Boiss. &amp; Reut

Fakir, Loubna El; Bouothmany, Kaoutar; Alotaibi, Amal; Bourhia, Mohammed; Ullah, Riaz; Zahoor, Saira; Mzibri, Mohamed El; Ghalem, Saïd; Alaoui, Tajelmolk; Zaîd, Abdelhamid; Benbacer, Laïla

doi:10.3390/app11083510

Cited by 17 publications

(10 citation statements)

References 25 publications

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“…This extract showed substantial anticancer efficacy against human breast cancer MCF-7 and MDA-MB-231 ( Isbilen and Volkan, 2021 ). 1,2-benzenedicarboxylic acid was also reported to be present within the methanolic bark extract of Berberis hispanica and also showed anticancer potential against human breast cancer (MCF-7 and MDA-MB-231 cells) and human prostate cancer LnCap and 22 RV1 cells ( El Fakir et al, 2021 ). Similarly, dodecane was also reported from the GC-MS analysis of endophytic fungi Talaromyces purpureogenus extracts and showed its anticancer efficacy against HPV18 + human cervical cancer HeLa cells ( Kumari et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

In Vitro Evaluation of Antioxidant, Anticancer, and Anti-Inflammatory Activities of Ethanolic Leaf Extract of Adenium obesum

et al. 2022

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Adenium obesum commonly known as “desert rose” belongs to the family Apopcynaceae and has previously been reported for its anti-influenza, antimicrobial, and cytotoxic efficacies and well-known for their ethno-medicinal applications. In the present study, ethanolic extracts of A. obesum (AOE) were analyzed by gas chromatography-mass spectrometry (GC–MS) to identify the important phytochemical compounds. The GC–MS analysis of AOE detected the presence of 26 phytochemical compounds. This plant is traditionally used for the treatment of various diseases. In this report, the antioxidant, anti-inflammatory, and anticancer activities of ethanolic leaf extract from A. obesum (AOE) were studied. The antioxidant potential of ethanolic extract of AOE was examined by different antioxidant assays, such as antioxidant capacity by the DPPH, ABTS, superoxide, hydroxyl radical scavenging, and lipid peroxidation inhibition assays. The antioxidant activities of various reaction mixtures of AOE were compared with a reference or standard antioxidant (ascorbic acid). In addition, we also evaluated the anticancer activity of AOE, and it was observed that AOE was found to be cytotoxic against A549 lung cancer cells. It was found that AOE inhibited the viability of A549 lung cancer cells by inducing nuclear condensation and fragmentation. Furthermore, ethanolic AOE demonstrated the anti-inflammatory potential of AOE in murine alveolar macrophages (J774A.1) as an in vitro model system. AOE showed its potential in reducing the levels of inflammatory mediators including the proinflammatory cytokines and TNF-α. The results obtained in the present investigation established the antioxidant, anticancer, and anti-inflammatory potency of AOE, which may account for subsequent studies in the formulation of herbal-based medicine.

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Section: Discussionmentioning

confidence: 99%

In Vitro Evaluation of Antioxidant, Anticancer, and Anti-Inflammatory Activities of Ethanolic Leaf Extract of Adenium obesum

et al. 2022

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“… 17 It has been reported that after an incubation period of 72hrs, methanolic extract of Berberis hispanica showed a 50% reduction in the proliferation of MCF-7 cells. 18 One more study reported the anti-proliferative effect of Zaleya pentandra extract against MCF-7 cells almost 37% after 24hrs of treatment. 19 While another study reported the anti-proliferative effect of Allium autumnale extract which showed 98.5%, 99.68%, and 99.77% reduction in MCF-7 cells growth after 24hrs, 48hrs, and 72hrs of treatment, respectively.…”

Section: Discussionmentioning

confidence: 99%

Anticancer effect of Illicium verum (star anise fruit) against human breast cancer MCF-7 cell line

Pahore¹,

Khan

Karim³

2022

Pak J Med Sci

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Objective: To investigate the anticancer effect of Illicium verum against human breast cancer MCF-7 cell line. Methods: An experimental study was conducted in Multidisciplinary and Tissue Culture Laboratory, Aga Khan University in collaboration with Pharmacology Department of Bahria University Medical and Dental College, Karachi, Pakistan from January 2021 to June 2021. MCF-7 cells of Luminal-A breast cancer were seeded in 96-well plate and treated with I.verum methanol extract. After incubation, MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) dye was used for cell viability and cell proliferation assays to determine the number of dead and viable cells, and the absorbance was measured using an enzyme-linked immunosorbent assay (ELISA) plate reader. In cell viability assay, different doses of I. verum methanol extract were used to treat the MCF-7 (0.25, 0.5, 1, 3, 6, 12, 25, and 50µg/ml) cells. For apoptosis analysis, the cells were processed with 4´, 6-diamidino-2-phenylindole fluorescent nuclear dye (DAPI) and were examined for fluorescence intensity and apoptotic cells. For cell proliferation assay and apoptosis the IC50 dose of 5.5µg/ml I. verum methanol extract was used. Results: The MCF-7 cells showed a significant reduction (p-value <0.01) in cell viability in the presence of all tested doses of I. verum methanol extract, except for the dose of 0.25µg/ml. The IC50 dose 5.5µg/ml of same extract also showed a significant reduction (p-value <0.01) in cell proliferation and apoptosis induction in MCF-7 cells. Conclusions: Illicium verum methanol extract possesses very potent anticancer action against MCF-7 cells through cytotoxicity, reduction, and inhibition of cancer cells and by inducing apoptosis. doi: https://doi.org/10.12669/pjms.39.1.6580 How to cite this: Pahore AK, Khan S, Karim N. Anticancer effect of Illicium verum (star anise fruit) against human breast cancer MCF-7 cell line. Pak J Med Sci. 2023;39(1):---------. doi: https://doi.org/10.12669/pjms.39.1.6580 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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“…About 60% of medications allowed for cancer treatment are of a natural source. The anticancer activity was not reported and published by other researchers to date, but different species of genus Berberis are reported to have in vitro cytotoxic activity against different destructive cell lines (El Fakir et al, 2021;Ilyas et al, 2021). An evaluation of the anticancer activity of the ethanolic extracts of the bark, leaves, and roots of the B. baluchistanica plant was done by using MTT cell assay against HeLa cell line.…”

Section: Discussionmentioning

confidence: 99%

Elucidating Therapeutic and Biological Potential of Berberis baluchistanica Ahrendt Bark, Leaf, and Root Extracts

2022

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Berberis baluchistanica Ahrendt is a medicinal plant known to have potential for the treatment of various diseases. In the present study, the ethanolic extracts of the bark, leaves, and roots of B. baluchistanica plant were evaluated for in vitro antimicrobial, anti-leishmanial, anticancer, and anti-inflammatory activities. The antibacterial and antifungal activities were determined by agar mix and agar well diffusion method. All extracts showed potential activity against the target bacteria (Bacillus subtilis, Bacillus licheniformis, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Rhodococcus erythropolis, Salmonella typhi, and Staphylococcus aureus) and fungal strains (Aspergillus flavus, Aspergillus niger, and Mucor mucedo). S. aureus proved to be the most sensitive strain for each extract, with a maximum zone of inhibition for bark at 23 ± 0.12 mm, for leaves at 22 ± 0.36 mm, and for root extracts at 20.21 ± 0.06 mm). The minimum inhibitory concentration values of B. baluchistanica bark, leaves, and roots for different target bacterial strains ranged from 1.56 to 25 mg ml–1, and the minimum bactericidal concentrations were in the range of 3.12 to 25 mg ml–1, respectively. The root extract possessed potent antifungal activity against A. flavus with 83% of growth inhibition, A. niger with 80%, and M. mucedo with 73%. The bark extract was found active against M. mucedo with 86% of inhibition, followed by 70% against A. flavus and 60% against A. niger. The leaf extract showed a significant response by 83% inhibition against M. mucedo, followed by A. flavus and A. niger with 73 and 72% inhibition, respectively. In an anti-leishmanial bioassay, the inhibitory concentration (IC50) was observed for each extract against Leishmania major. The bark showed good activity (IC50 = 4.95 ± 0.36 mg/ml), followed by the roots (IC50 = 7.07 ± 0.18 mg/ml) and the leaves (IC50 = 8.25 ± 0.29 mg/ml). An evaluation of anticancer activity was done by using MTT cell assay against HeLa cell line. Upon comparing the values of each extract to the standard, it was revealed that the ethanolic bark extract showed the highest anticancer activity with IC50 = (12 ± 0.15 μg/ml), followed by the roots (14 ± 0.15 μg/ml) and the leaves (17 ± 0.21 μg/ml), respectively. The anti-inflammatory assay was undertaken by the inhibition of albumin denaturation activity, proteinase inhibitory activity, and heat-induced hemolysis activity. The IC50 value for protein denaturation of the bark was IC50 = 0.64 ± 0.25 mg/ml, followed by the roots (0.67 ± 0.21 mg/ml) and the leaves (0.73 ± 0.13 mg/ml). The proteinase inhibitory activity of the bark extract was IC50 = 0.55 ± 0.12 mg/ml, followed by the leaves (0.62 ± 0.23 mg/ml) and the roots (0.69 ± 0.15 mg/ml), respectively. For heat-induced hemolysis assay, the bark showed the lowest IC50 value (0.48 ± 0.15 mg/ml) compared to the leaves (0.52 ± 0.35 mg/ml) and the roots (0.58 ± 0.05 mg/ml) of the plant. All analyzed parts of the B. baluchistanica plant showed significant biological activities which make the plant medicinally important and a good candidate for the isolation of antimicrobial, inflammatory, and anticancer compounds. Further studies may lead us to determine the active compounds responsible for the biological activities of the plant extracts.

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Antioxidant and Understanding the Anticancer Properties in Human Prostate and Breast Cancer Cell Lines of Chemically Characterized Methanol Extract from Berberis hispanica Boiss. & Reut

Cited by 17 publications

References 25 publications

In Vitro Evaluation of Antioxidant, Anticancer, and Anti-Inflammatory Activities of Ethanolic Leaf Extract of Adenium obesum

In Vitro Evaluation of Antioxidant, Anticancer, and Anti-Inflammatory Activities of Ethanolic Leaf Extract of Adenium obesum

Anticancer effect of Illicium verum (star anise fruit) against human breast cancer MCF-7 cell line

Elucidating Therapeutic and Biological Potential of Berberis baluchistanica Ahrendt Bark, Leaf, and Root Extracts

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