Antioxidant, anticancer and antimicrobial effects of In vitro developed protocorms of Dendrobium longicornu

Abstract

Highlights In vitro protocorms development for Dendrobium longicornu was successfully established on endophytic elicitor supplemented MS-medium. Methanol extract of protocorms biomass was evaluated for its antioxidant, anticancer and antimicrobial effects. Bioactive secondary metabolites present in methanol extract were identified by GC–MS technique. Methanol extract showed moderate antioxidant, anticancer and antimicrobial effects. Protocorms of this plant are the good source of bioactive compounds for the industrial applications.

Analysis of Streptomyces Volatilomes Using Global Molecular Networking Reveals the Presence of Metabolites with Diverse Biological Activities

“…The production of some plant-associated metabolites and VOCs in Streptomyces has been observed previously ( 18 , 52 ), highlighting the potential for using bacteria to produce such compounds. In addition, VOCs annotated with high confidence using MSHub/GNPS are reported to display a variety of activities, including antibacterial [1-tridecanol, 3-pentadecanol, and bis(2-ethylhexyl) phthalate] ( 53 – 62 ), antifungal [cis-9-hexadecenal, (Z)-12-octadecenoic acid methyl ester; hexanedioic acid; bis(2-ethylhexyl) ester and octadecyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl) propionate] ( 63 – 69 ), and anticancer [6,10-dimethyl-4-undecanol and 1,2-benzenedicarboxylic acid bis(2-ethylhexyl) ester] ( 70 , 71 ). Bis(2-ethylhexyl) phthalate is often considered a background artifact during the analysis of biologically produced volatiles but has been reported to be produced by many organisms, and there have been some discussions regarding it being classified as a natural product ( 62 ).…”

“…4 ), but the conventional approach annotated some compounds that MSHub/GNPS missed, which included geosmin, cubenol, benzyl alcohol, 1 H -benzocycloheptene, and 4a S -cis-2,4a,5,6,7,8,9,9a-octahydro-3,5,5-trimethyl-9-methylene, all of which have insect repellent activities ( 51 – 54 , 63 , 64 ). In comparison, MSHub/GNPS was able to annotate some VOCs with antibacterial [1-tridecanol, 3-pentadecanol, and bis(2-ethylhexyl) phthalate] ( 53 , 54 ), antifungal [cis-9-hexadecenal, (Z)-12-octadecenoic acid methyl ester, hexanedioic acid, bis(2-ethylhexyl) ester, and octadecyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl)propionate] ( 63 – 69 ), and anticancer [6,10-dimethyl-4-undecanol and 1,2-benzenedicarboxylic acid bis(2-ethylhexyl) ester] ( 70 , 71 ) activities, which were missed by the conventional method. Results suggest that MSHub/GNPS and the conventional method are complementary due to obvious differences in design and workflow, and that the use of both methods is required for thorough volatilome characterization.…”

Comparative Cytotoxic Activity of Wild Harvested Stems and In Vitro-Raised Protocorms of Dendrobium chryseum Rolfe in Human Cervical Carcinoma and Glioblastoma Cell Lines

“…D. longicornu and D. moniliforme showed cytotoxic activities against HeLa and U251 cancer cell lines [ 4 , 5 , 35 ]. Bulbophyllum kwangtungense showed antitumor activities against HeLa and K562 human tumor cell lines [ 53 ] while Bulbophyllum odoratissimum was found to have cytotoxic activities against human cancer cell lines, such as human leukemia cell lines K562 and HL-60, human hepatoma BEL-7402, human lung adenocarcinoma A549, and human stomach cancer cell line SGC-7901 [ 54 ]. Prior to this study, no comparative investigation of the cytotoxic activity of plants grown in vitro versus wild plants has been reported.…”

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“…Antioxidants also show antitumour, antimicrobial, and cytotoxic effects [51][52][53][54][55] . A study on the antioxidant potential in relation to total phenolics, flavonoids, anthocyanins and carotenoids was reported in the whole kernels of different wheat genotypes 56 .…”