Antioxidant biocompatible composite collagen dressing for diabetic wound healing in rat model

Abstract

Associated with persistent oxidative stress, altered inflammatory responses, poor angiogenesis and epithelization, wound healing in diabetic patients is impaired. N-acetylcysteine (NAC) is reported to resist excess reactive oxygen species (ROS) production, prompt angiogenesis and maturation of the epidermis. Studies have revealed that graphene oxide (GO) can regulate cellular behavior and form cross-links with naturally biodegradable polymers such as collagen (COL) to construct composite scaffolds. Here, we reported a COL-based implantable scaffold containing a mixture of GO capable of the sustained delivery of NAC to evaluate the wound healing in diabetic rats. The morphological, physical characteristics, biocompatibility and NAC release profile of the GO-COL-NAC (GCN) scaffold were evaluated in vitro. Wound healing studies were performed on a 20 mm dorsal full-skin defect of streptozotocin (STZ)-induced diabetic rats. The injured skin tissue was removed at the 18th day post-surgery for histological analysis and determination of glutathione peroxidase (GPx), catalase (CAT) and superoxide dismutase (SOD) activity. In diabetic rats, we confirmed that the GCN scaffold presented a beneficial effect in enhancing the wound healing process. Additionally, due to the sustained release of NAC, the scaffold may potentially induce the antioxidant defense system, upregulating the expression levels of the antioxidant enzymes in the wound tissue. The findings revealed that the antioxidant biocompatible composite collagen dressing could not only deliver NAC in situ for ROS inhibition but also promote the wound healing process. This scaffold with valuable therapy potential might enrich the approaches for surgeon in diabetic wound treatment in the future.

Photocrosslinking silver nanoparticles–aloe vera–silk fibroin composite hydrogel for treatment of full-thickness cutaneous wounds

“…As shown in Fig. 3a , C–N stretching (1451.88 cm −1 ) in the SF transferred into the new C–N (1017.68 cm −1 ); at the same time, the intensity of C–O (3339.11 cm −1 ), C = O (1634.70 cm −1 and 1249.80 cm −1 ) and N–H stretching (1543.73 cm −1 ) in the Ag–AV–SF system significantly improved compared with that in the SF [ 25 ]. The phenomenon of stretching vibration was attributed to the α-amino nitrogen and α-carboxylic oxygen from the tyrosine formed complexes with silver ions through coordination bonds [ 33 ].…”

“…Thermogravimetric analysis (TGA) is a physicochemical property that could be treated as the colloidal stability of the hydrogel [ 24 , 25 ]. TGA was performed using a thermal gravimetric analyzer (TA SDT 650, TA, USA).…”

Rescuing Nucleus Pulposus Cells From Senescence via Dual‐Functional Greigite Nanozyme to Alleviate Intervertebral Disc Degeneration

“…[26] In recent years, NAC showed extensive and far-reaching potential in the treatment of ROS-related kidney injury, myocardial injury, diabetes-related wound healing and so on. [68][69][70][71][72] In addition, NAC appears as a raw material in the synthesis of nanomaterials for a wide application, which has dabbled in the fields of drug delivery, medical imaging and so on. [73][74][75] In the microenvironment of intervertebral disc degeneration, the accumulation of ROS triggers a series of problems including mitochondrial dysfunction, NPC senescence, and the continued secretion of SASP.…”

Oxidative Stress Level as a Predictor of Anastomotic Leakage after Rectal Surgery

“…Our study found these indicators have a similar AL predictive effect as CRP. SOD and CAT are antioxidant enzymes, which constitute the primary cellular antioxidant defenses [ 43 ]. Studies have reported that the rise of SOD, CAT, and other antioxidant enzymes is an indication of tissue repair [ 44 ].…”