Three major cultivars of strawberries ('Yukbo,' 'Seolhyang,' and 'Janghee') harvested from three different locations (Gyeongsan, Nonsan, and Daegu) in Korea were used for this study. The soluble solid content of 'Yukbo' harvested from Nonsan was the highest among the samples. The ranges of titratable acidity and pH from the samples were 0.48-0.65 and 3.70-4.01 %, respectively. Fructose, glucose, and citric acid contents of 'Janghee' harvested from Daegu were significantly higher than other strawberries (p \ 0.05). The ascorbic acid contents of 'Seolhyang' and 'Janghee' grown in Nonsan were significantly higher than in other strawberries (p \ 0.05). Total flavonoid and phenolic concentrations, and total antioxidant activities of 'Janghee' harvested from Daegu were significantly higher than other samples (p \ 0.05). Total flavonoids and total phenolics were highly correlated with the antioxidant activities, and the relationships between total flavonoids and total phenolics were also strong. Cultivars and harvest locations may affect to the physicochemical quality and antioxidant activity of the fruit.