Antioxidant Effects, Antiproliferative Effects, and Molecular Docking of Clinacanthus nutans Leaf Extracts

Ismail, Noor Zafirah; Toha, Zaleha Md; Muhamad, Musthahimah; Kamal, Nik Nur Syazni Nik Mohamed; Zain, Nur Nadhirah Mohamad; Arsad, Hasni

doi:10.3390/molecules25092067

Cited by 29 publications

(15 citation statements)

References 64 publications

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“…In this study, fraction solvents were consecutively fractionated from the crude methanolic extract due to the fact that fraction extracts may have high inhibition effects against cancer cells [19]. The extraction yields of C. vespertilionis extracts were in line with the study by Ismail et al [20], which showed the similar results with the percentage yield of Clinacanthus nutans extracts. CV-Aqu extract was best at achieving higher extraction than other solvents.…”

Section: Discussionsupporting

confidence: 77%

Christia vespertilionis extract induced antiproliferation and apoptosis in breast cancer (MCF7) cells

et al. 2021

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Background: C. vespertiliomis extracts were evaluated for antiproliferative and apoptosis effect on breast cancer (MCF7) cells.Methods and Results: The leaves extracts were analysed for its antiproliferative effect on breast cancer (MCF7) cells and normal epithelial breast (MCF 10A) cells using Sulforhodamine B (SRB) assay. The selective extract was evaluated for its ability to induce apoptosis using Annexin V-FITC apoptosis staining and the expression of molecular genes using qualitative reverse transcription-polymerase chain reaction (RT-PCR) against MCF7 cells. Gas chromatography-mass spectrometry (GC-MS) was used to identify the compounds from the selective extract. The ndings showed that dichloromethane fraction (CV-Dcm) extract had high antiproliferative effect against MCF7 cells (IC 50 = 24 µg/mL, selective index (SI) = 8.17). The percentages of apoptosis cells in CV-Dcm-treated MCF7 cells was 58.8%. The CV-Dcm extract induced downregulation of PCNA level. The apoptotic genes were also triggered in both extrinsic and intrinsic signaling pathways, affecting a 1.5-fold increase in BAX, 1.4-fold increase in cytochrome c, 1.3-fold increase in caspase-8, 1.7-fold increase in caspase-3 and 0.5-fold-decrease in BCL-2. Treated MCF7 cells also activated P53-dependent apoptotic death pathway.Conclusions: The present work strongly suggests that high e cacy of CV-Dcm extract was attributed to its antiproliferative and apoptosis-inducing activation in MCF7 cells, most likely due to its favourable compounds.

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Section: Discussionsupporting

confidence: 77%

Christia vespertilionis extract induced antiproliferation and apoptosis in breast cancer (MCF7) cells

et al. 2021

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“…Nevertheless, Oleic and Palmitic (n-Hexadecanoic) fatty acids were the two main components of the aqueous extract occupied a peak area of 32.64% and 26.35%, respectively (Table 1). Interestingly, both active compounds are fatty acids that have been characterized with anti-inflammatory (Aparna, 2012; Korbecki and Bajdak-Rusinek, 2019), anticancer (Ismail, 2020), antioxidant (Vaithiyanathan and Mirunalini, 2015), antibacterial and antifungal activities (Ogunlesi, 2009). Nona-and Pentadecane existed in smaller amounts of 4.34% and 3.25%, and both characterized with antioxidant, anti-inflammatory (Ali et al, 2015), and antimicrobial activity (Girija, 2014), respectively.…”

Section: Aqueous Extractmentioning

confidence: 99%

“…However, n-Hexadecanoic and 9-Octadecenoic acids were the two most abundant components of the dry roots aqueous extract with a peak area of 27.69% and 27.28%, respectively (Table 2). Recently, several reports pointed to the substantial potent of such fatty acids, particularly as an anti-inflammatory (Aparna, 2012; Korbecki and Bajdak-Rusinek, 2019), anticancer (Ismail, 2020), antioxidant (Vaithiyanathan and Mirunalini, 2015), antibacterial and antifungal properties (Ogunlesi, 2009). Diterpenoid in the form of 2,6,10,14-Hexadecatetraen-1-ol, 3,7,11,15-tetramethyl-, acetate, (E,E,E), (4.37 %) was detected in the dry roots aqueous extract known with various pharmacological activities like antimicrobial, antiinflammatory, anticancer and diuretic activities (Devi and Muthu, 2015).…”

Section: Aqueous Extractmentioning

confidence: 99%

BIOCHEMICAL CHARACTERIZATION OF Ferula rutbaensis MEDICINAL PLANT IN IRAQI WESTERN DESERT

Dawood¹,

Alfalahi²,

Neamah³

et al. 2020

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Plants used in folk medicine not only represent rich sources for therapeutic materials, but it also plays a crucial role in developing completely or partially novel synthesized drugs. Mharut plant (Ferula rutbaensis) is an integral part of Bedouin therapeutic practices in the western desert of Anbar province-Iraq. Still, to date, this is the first study describing its phytochemical constituents. The plant was growing near the Iraq-Saudi Arabia borders and adapted to a wide range of soils. Traditionally, F. rutbaensis has been widely used to treat acne, stomach and bowel disorders, food poisoning and respiratory problems. Fresh plant samples were collected and morphologically characterized. Likewise, the ITS-based DNA barcoding technique was efficiently used to approve the morphological identification of F. rutbaensis. The GC-MS spectrum was adopted in the phytochemical characterization of aqueous and methanol extracts of fresh and dry plant parts. The aqueous extract of dry roots was the richest source for bioactive compounds than fresh or methanolic extracts of either fresh or dry plant parts. In general, the detected phytochemicals falling into fatty acids, terpenes, hydrocarbon alkanes, and esters. Notably, fatty acids in Oleic and Palmitic acids were the two most abundant bioactive compounds in both aqueous and methanolic extracts of plant fresh and dry roots. The detected unsaturated fatty acids and/or other bioactive components are laying behind the therapeutic properties of F. rutbaensis that can be useful ingredients to prepare Mharut-based cosmetics such as medical soaps, body lotions, skin conditioners and sunscreens. Additionally, some other components were found to have anti-inflammatory, antioxidants, and antimicrobial properties. Further investigations will be necessary to confirm the antimicrobial activity of F. rutbaensis extracts.

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“…This shows that C. nutans leaf extracts exerted an antioxidant activity in the 4-T1 mouse breast model [ 40 ]. Likewise, a study lead by [ 41 ] has used C. nutans leaves extracted with 80% methanol and further fractionated with n-hexane, dichloromethane, chloroform, n -butanol and aqueous residue ranging between 125 and 4000 µg/mL, whereas the total flavonoid content, total phenolic content and total antioxidant scavenging activity on breast cancer (Michigan Cancer Foundation-7 [MCF7]) and normal breast (Michigan Cancer Foundation-10A [MCF 10A]) cell lines were measured using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical cation decolourization assay. Based on the findings, the total phenolic content in C. nutans leaf extracts was higher than total flavonoid content.…”

Section: Pharmacological and Medicinal Propertiesmentioning

confidence: 99%

A Narrative Review on the Phytochemistry, Pharmacology and Therapeutic Potentials of Clinacanthus nutans (Burm. f.) Lindau Leaves as an Alternative Source of Future Medicine

et al. 2021

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The application of natural products and supplements has expanded tremendously over the past few decades. Clinacanthus nutans (C. nutans), which is affiliated to the Acanthaceae family, has recently caught the interest of researchers from the countries of subtropical Asia due to its medicinal uses in alternative treatment for skin infection conditions due to insect bites, microorganism infections and cancer, as well as for health well-being. A number of bioactive compounds from this plant’s extract, namely phenolic compounds, sulphur containing compounds, sulphur containing glycosides compounds, terpens-tripenoids, terpens-phytosterols and chlorophyll-related compounds possess high antioxidant activities. This literature search yielded about one hundred articles which were then further documented, including the valuable data and findings obtained from all accessible electronic searches and library databases. The promising pharmacological activities from C. nutans leaves extract, including antioxidant, anti-cancer, anti-viral, anti-bacterial, anti-fungal, anti-venom, analgesic and anti-nociceptive properties were meticulously dissected. Moreover, the authors also discuss a few of the pharmacological aspect of C. nutans leaves extracts against anti-hyperlipidemia, vasorelaxation and renoprotective activities, which are seldom available from the previously discussed review papers. From the aspect of toxicological studies, controversial findings have been reported in both in-vitro and in-vivo experiments. Thus, further investigations on their phytochemical compounds and their mode of action showing pharmacological activities are required to fully grasp both traditional usage and their suitability for future drugs development. Data related to therapeutic activity and the constituents of C. nutans leaves were searched by using the search engines Google scholar, PubMed, Scopus and Science Direct, and accepting literature reported between 2010 to present. On the whole, this review paper compiles all the available contemporary data from this subtropical herb on its phytochemistry and pharmacological activities with a view towards garnering further interest in exploring its use in cardiovascular and renal diseases.

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Antioxidant Effects, Antiproliferative Effects, and Molecular Docking of Clinacanthus nutans Leaf Extracts

Cited by 29 publications

References 64 publications

Christia vespertilionis extract induced antiproliferation and apoptosis in breast cancer (MCF7) cells

Christia vespertilionis extract induced antiproliferation and apoptosis in breast cancer (MCF7) cells

BIOCHEMICAL CHARACTERIZATION OF Ferula rutbaensis MEDICINAL PLANT IN IRAQI WESTERN DESERT

A Narrative Review on the Phytochemistry, Pharmacology and Therapeutic Potentials of Clinacanthus nutans (Burm. f.) Lindau Leaves as an Alternative Source of Future Medicine

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