Antioxidant effects of chrysin-loaded electrospun nanofibrous mats on proliferation and stemness preservation of human adipose-derived stem cells

Abstract

An ideal biomaterial in regenerative medicine should be able to regulate the stem cell proliferation without the loss of its pluripotency. Chrysin (Chr) is a naturally occurring flavone with a wide spectrum of biological functions including anti-inflammatory and anti-oxidant properties. The present study describes the influence of Chr-loaded nanofibrous mats on the regulation of proliferation and stemness preservation of adipose-derived stem cells (ADSCs). For this purpose, Chr-loaded poly (ε-caprolactone)/poly (ethylene glycol) (PCL/PEG) nanofibrous mats were produced via electrospinning process and the successful fabrication of these bioactive mats was confirmed by field emission scanning electron microscopy (FE-SEM) and fourier transform infrared spectroscopy. ADSCs were seeded on the nanofibers and their morphology, viability, and stemness expression were analyzed using FE-SEM, MTT, and qPCR assays after 2 weeks of incubation, respectively. The results display that ADSCs exhibit better adhesion and significantly increased viability on the Chr-loaded PCL/PEG nanofibrous mats in relative to the PCL/PEG nanofibers and tissue culture polystyrene. The greater viability of ADSCs on Chr based nanofibers was further confirmed by higher expression levels of stemness markers Sox-2, Nanog, Oct-4, and Rex-1. These findings demonstrate that Chr-loaded PCL/PEG electrospun nanofibrous mats can be applied to improve cell adhesion and proliferation while concurrently preserving the stemness of ADSCs, thus representing a hopeful potential for application in stem cell therapy strategies.

Curcumin Affects Adipose Tissue-Derived Mesenchymal Stem Cell Aging Through TERT Gene Expression

“…The use of stem cells from different tissues for regenerative medicine applications has increased over the past few years [21][22][23][24]. Similar to normal somatic cells, adult stem cells are exposed to stressors during their life span, leading to an age-dependent decline in their number and function [25].…”

Spotlight on 17‐AAG as an Hsp90 inhibitor for molecular targeted cancer treatment

“…In recent years, advances in nanomaterials‐based drug delivery carriers have paved the way for overcoming these obstacles (Deldar, Zarghami, Pilehvar‐Soltanahmadi, Dadashpour, & Zarghami, ; Farajzadeh et al., ; Montazeri et al., ). The main advantages of nanocarriers for anticancer drugs include improved therapeutic effect, extended half‐life in the bloodstream, increased water solubility, improved stability, selective delivery to specific site, and decreased hepatotoxicity (Firouzi‐Amandi et al., ; Jalilzadeh‐Tabrizi et al., ; Nejati‐Koshki, Mortazavi, Pilehvar‐Soltanahmadi, Sheoran, & Zarghami, ; Zamani, Pilehvar‐Soltanahmadi, Alizadeh, & Zarghami, ).…”

“…In addition 17-AAG delivered via DMSO or Cremophor EL has a short half-life, limited ability to reach target cells, induction of drug resistance, poor specificity, and high hepatotoxicity that restrict its clinical use (Shin & Kwon, 2017). In recent years, advances in nanomaterials-based drug delivery carriers have paved the way for overcoming these obstacles (Deldar, Zarghami, Pilehvar-Soltanahmadi, Dadashpour, & Zarghami, 2017;Montazeri et al, 2017). The main advantages of nanocarriers for anticancer drugs include improved therapeutic effect, extended half-life in the bloodstream, increased water solubility, improved stability, selective delivery to specific site, and decreased hepatotoxicity (Firouzi-Amandi et al, 2018;Jalilzadeh-Tabrizi et al, 2018;Nejati-Koshki, Mortazavi, Pilehvar-Soltanahmadi, Sheoran, & Zarghami, 2017;Zamani, Pilehvar-Soltanahmadi, Alizadeh, & Zarghami, 2018).…”

Antioxidant Small Molecule Compound Chrysin Promotes the Self-Renewal of Hematopoietic Stem Cells

“…In recent years, there are a growing number of researches on chrysin and its derivatives. Some progress has been made in the effect of chrysin on the promotion of mesenchymal stem cell proliferation and maintenance of human adiposederived stem cells stemness (Deldar et al, 2017;Menon et al, 2018). However, in the field of hematopoiesis, the effect of chrysin on HSCs has not been reported.…”