Antioxidant Evaluation and Phytochemical Content of Various Rice Bran Extracts of Three Varieties Rice From Semarang-Central Java, Indonesia

Abstract

Objectives:The objectives of this research were to evaluate antioxidant activity from different polarities rice bran extract of three varieties of rice using two methods of antioxidant testing which were ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH), and correlation of total phenolic, flavonoid and carotenoid content with their exhibitory concentration 50 (EC 50 ) of FRAP and inhibitory concentration 50 (IC 50 ) of DPPH antioxidant activities.Methods: Extraction was conducted by reflux using different polarity solvents. The extracts were evaporated using rotary evaporator. Determination of total phenolic, flavonoid and carotenoid content, antioxidant activities using FRAP and DPPH assays was performed by ultraviolet-visible spectrophotometry and its correlation with EC 50 of FRAP capacities and IC 50 of DPPH scavenging activities was analyzed by Pearson's method. Results:Ethanolic rice bran extract of black rice showed the lowest EC 50 of FRAP capacity 64.35 µg/ml and IC 50 of DPPH scavenging activity 23.92 µg/ml. The highest phenolic content, flavonoid content, and carotenoid content were also given by ethanolic rice bran extract of black rice. There was significantly negative correlation between total phenolic content and carotenoid content in rice bran extract of red rice and black rice with their IC 50 of DPPH. Conclusions:All of the rice bran extracts (except n-hexane rice bran extract of black rice and ethanolic rice bran extract of white rice) were very strong antioxidant by DPPH assay. Phenolic and carotenoid compounds in rice bran extracts of red rice and black rice were the major contributor in antioxidant activity by DPPH assay. Rice bran extracts of black rice had linear results by FRAP and DPPH assays.

Pengembangan Sereal Sarapan Tersubstitusi Bekatul dan Tepung Pisang

“…Nilai aktivitas antioksidan dan IC50 pada sereal sarapan terpilih berturut-turut adalah 40,67 ± 1,71 mg AEAC/100 g dan 84,48 ± 0,99 mg/mL. IC50 (inhibition concentration) merupakan konsentrasi sampel yang dibutuhkan untuk menghambat radikal bebas DPPH sebesar 50% di mana nilai IC50 terendah menunjukkan aktivitas antioksidan tertinggi (Sukrasno et al 2017). Nilai IC50 pada bekatul beras varietas IR 64 adalah 0,35 mg/mL (Sugiat et al 2010).…”

Ethnopharmacognosy study, antioxidant activity, and chemical content in chicken bile

“…The IC50 value is determined through the linear regression equation from the calibration curve, namely the percentage of curing as the y-axis and the concentration of antioxidants as the x-axis. The IC50 value is calculated by entering the 50% value into the regression equation as the y value, then the x value is calculated as the IC50 concentration (Sukrasno et al, 2017).…”

Ethanolic Extract of Tubtim-Chumphae Rice Bran Decreases Insulin Resistance and Intrahepatic Fat Accumulation in High-Fat-High-Fructose Diet Fed Rats

“…Various types of rice (Oryza sativa) are widely cultivated around the world. Rice is not only a major source of energy but also a source of many bioactive compounds and especially antioxidants including phenolic compounds [6][7][8]. Rice bran, a byproduct of rice grain milling process, is also full of many valuable bioactive compounds such as Vitamin E, γ-oryzanol, and anthocyanins [9] with health beneficial effects in decreasing blood cholesterol and reducing the incidence of atherosclerotic disease [10].…”