Antioxidant, genotoxic and lysosomal biomarkers in the freshwater bivalve (Unio pictorum) transplanted in a metal polluted river basin

Guidi, Patrizia; Frenzilli, Giada; Benedetti, Maura; Bernardeschi, Margherita; Falleni, Alessandra; Fattorini, Daniele; Regoli, Francesco; Scarcelli, Vittoria; Nigro, Marco

doi:10.1016/j.aquatox.2010.07.009

Cited by 63 publications

(36 citation statements)

References 43 publications

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“…Previous relevant scientific literature from this region was unfortunately based on incorrect taxonomy; for these studies, covering a range of scientific fields, including ecology (e.g. Ravera and Sprocati 1997;Ravera et al 2007), physiology (Campanella et al 2005;Carella et al 2016), toxicology (Guidi et al 2010;Polettini et al 2015;Sabbioni et al 2015), and aquaculture (Panini et al 2008;Sicuro et al 2010Sicuro et al , 2015, reanalysis using the taxonomy and distribution revealed in the present study might be necessary. For example, the study species of Ravera and Sprocati (1997), Ravera et al (2007), and Sicuro et al (2010) is U. elongatulus rather than U. mancus, which is only present in southern Italy, and when describing the host fishes of Anodonta cygnea, Giusti et al (1975) were not aware that three Anodonta species occurred in Trasimeno Lake instead of one.…”

Section: Discussionmentioning

confidence: 99%

Lifting the curtain on the freshwater mussel diversity of the Italian Peninsula and Croatian Adriatic coast

et al. 2017

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Freshwater mussels of the order Unionida have been dramatically declining globally. Despite their ecological importance, conservation of these animals has been hindered by unresolved taxonomy and a lack of data on the distribution and status of populations, especially in southern Europe. Although the Italian Peninsula has been noted as a centre of endemism and one of the major refugia of the glacial ages for several taxa, few studies have been performed on the genetic diversity of Unionida. Most importantly, the taxonomic status of several freshwater mussel populations of the Italian Peninsula is still unresolved. Here we present the first comprehensive dataset for the Unionida of the region spanning Italy and the coastal Croatian region (west of the Dinaric Alps). In total, Communicated by Robert Cowie.This article belongs to the Topical Collection: Coastal and marine biodiversity. (COI, 16S and 28S). Genetic analyses were performed to understand major phylogenetic and phylogeographic patterns. Seven species were detected: three Anodonta species (A. anatina, A. cygnea and A. exulcerata), two Unio species (U. mancus and U. elongatulus), Microcondylaea bonellii, and the invasive Sinanodonta woodiana. The presence of three endemic species (A. exulcerata, U. elongatulus and M. bonellii) confirms the importance of the region as a centre of endemism for freshwater mussels. The Apennine Mountains act as an important biogeographic barrier. Electronic supplementary material

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Section: Discussionmentioning

confidence: 99%

Lifting the curtain on the freshwater mussel diversity of the Italian Peninsula and Croatian Adriatic coast

et al. 2017

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“…The micronuclei test is considered to be a sensitive and informative marker of cytogenetic damage caused by mutagenic compounds (Arslan et al, 2015) and has been applied to identify the adverse potential of various genotoxic agents (Barsiene et al, 2012), both in freshwater and marine sentinel species (Guidi et al, 2010). The increase in MN frequency was observed in both treatment groups compared to the control.…”

Section: Discussionmentioning

confidence: 91%

Effects of manganese on fat snook Centropomus parallelus (Carangaria: Centropomidae) exposed to different temperatures

et al. 2017

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This study evaluates the effects of exposure to manganese (Mn 2+ ) for 96 hours at two different temperatures (24 and 27°C) on juveniles of Centropomus parallelus through the activities of glutathione S-transferase (GST) and catalase (CAT), micronuclei test (MN) and comet assay. The GST activity did not show any significant difference between the groups exposed to Mn 2+ and the respective control groups; in contrast, a major increase in the CAT activity was observed at 27°C in the group exposed to Mn 2+ compared to the control group. The genotoxic analyses showed that in all animals exposed to Mn 2+, the number of red cells with micronuclei increased significantly compared to the respective control groups. There was also a significant increase in the incidence of DNA damage in the groups exposed to Mn 2+. At a temperature of 24ºC, animals exposed to Mn 2+ had more DNA damage than those at 27°C. It is likely that the increase in temperature can also induce oxidative stress. Thus, we conclude that manganese is toxic to the fat snook juveniles, causing genotoxic damage, and when associated with an increase in temperature, manganese can also provoke an increase in oxidative stress.Keywords: Biomarkers, DNA damage, Enzymes, Genotoxicity, Metal, Micronuclei.Este estudo avaliou os efeitos da exposição ao manganês (Mn 2+ ), após 96 horas, a duas temperaturas (24 e 27°C) em juvenis de Centropomus parallelus por meio de análises bioquímicas (atividade das enzimas glutationa S-transferase (GST) e catalase (CAT)) e genotóxicas (teste do micronúcleo e ensaio cometa). A atividade da GST não mostrou diferença significativa entre os grupos expostos ao Mn 2+ e os seus respectivos grupos controle, enquanto que um aumento significativo na atividade da CAT foi observado a 27°C no grupo exposto ao Mn 2+ , quando comparado ao grupo controle. As análises genotóxicas mostraram que os animais expostos ao Mn 2+ tiveram aumento significativo na quantidade de células com micronúcleo em relação aos seus grupos de controles. Houve também aumento significativo na incidência de danos ao DNA nos grupos expostos a esse contaminante. Na temperatura de 24°C, os animais expostos ao Mn 2+ tiveram maior quantidade de danos no DNA em relação a 27°C. É provável que o aumento da temperatura também possa induzir o estresse oxidativo. Assim, concluímos que o manganês é tóxico para os juvenis de robalo, causando dano genotóxico, e quando associado a um aumento da temperatura, também pode provocar um aumento no estresse oxidativo.

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“…In this regard, marine organisms such as bivalves have indeed been extensively investigated as ecotoxicological indicator species, with assessments of lysosomal/autophagy impairment in their digestive organs amongst the key parameters (Domouhtsidou and Dimitriadis, 2001;Moore and Allen, 2002;Marigómez and BaybayVillacorta, 2003;McVeigh et al, 2006;Moore et al, 2006cMoore et al, , 2007Shaw et al, 2011). Lysosomal membrane stability, as measured by neutral red retention, is also a useful parameter in assessing sublethal stress response in freshwater inhabitants such as the social amoeba Dictyostelium discoideum (Sforzini et al, 2008), as well as the freshwater mussels Dreissena polymorpha (Giambérini and Cajaraville, 2005;Binelli et al, 2009a,b) and Unio pictorum (Guidi et al, 2010). The assay has also been applied to vertebrate species like the freshwater fish Prochilodus lineatus (Monteiro et al, 2011).…”

Section: Autophagy and Lysosomal Pathology In Environmental Stressmentioning

confidence: 99%

“…Binelli et al (2009a,b) Unio pictorum Freshwater mussel Assessment of multiple biomarkers, including lysosomal membrane stability, using Unio pictorum transplanted in a metal polluted river basin. Guidi et al (2010) Dictyostelium discoideum…”

Section: Dreissena Polymorphamentioning

confidence: 99%

Autophagy in response to environmental stresses: New monitoring perspectives

Tang

2016

Ecological Indicators

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Antioxidant, genotoxic and lysosomal biomarkers in the freshwater bivalve (Unio pictorum) transplanted in a metal polluted river basin

Cited by 63 publications

References 43 publications

Lifting the curtain on the freshwater mussel diversity of the Italian Peninsula and Croatian Adriatic coast

Lifting the curtain on the freshwater mussel diversity of the Italian Peninsula and Croatian Adriatic coast

Effects of manganese on fat snook Centropomus parallelus (Carangaria: Centropomidae) exposed to different temperatures

Autophagy in response to environmental stresses: New monitoring perspectives

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