Antioxidant Indexes and Immune Function of the Intestinal Flora of Compound Microecological Preparations

Sun, Wanzhen; Zhang, Li

doi:10.1155/2022/5498514

Cited by 11 publications

(10 citation statements)

References 18 publications

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“…Consequently, adding probiotics to animal diets can partially replace antibiotics in feed. Due to its green, nontoxic, safe, and effective properties, probiotic supplementation has been increasingly used in animal husbandry (Sun & Zhang, 2022). According to the types of microorganisms, they can be classified as Bacillus , Lactic acid bacteria , yeast, and so on.…”

Section: Introductionmentioning

confidence: 99%

Compound probiotics microcapsules improve milk yield and milk quality of dairy cows by regulating intestinal flora

Wu,

Chang,

Zhang

et al. 2024

Food Bioengineering

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To address the issues of probiotic activity loss during storage and feeding, as well as the limited efficacy of single probiotics, a solution was devised by embedding a mixture of Bacillus coagulans SN‐8 (SN‐8) and Saccharomyces boulardii SN‐6 (SN‐6) in a gel. The initial step involved screening the probiotic microcapsules' preparation method and wall material. Using sodium alginate and β‐cyclodextrin as composite wall material and chitosan as the outer coating material allowed for an embedding rate of 82.11% in composite probiotic microcapsules prepared by the air atomization method. Next, in vitro, simulated digestion experiments were conducted to determine the number of viable bacteria and the release rate of the microcapsules. The results showed that compared to the free strain, the mixed probiotic microcapsules retained a survival rate of 67.5% after 3 h of simulated gastric juice exposure and 70.56% after 42 days of storage at 4°C. This demonstrated higher survival rates and storage stability. The prepared probiotic microcapsules were then administered to dairy cows. 16S rDNA gene sequencing showed that consumption of the microcapsules reduced the number of harmful bacteria, such as Paeniclostridium, in the intestinal tract of dairy cows while accelerating the growth of beneficial bacteria, such as Bifidobacterium. In particular, this resulted in a significant improvement in the lactation performance of the cows, with a 4.5% increase in milk fat content, a 92.5% increase in milk protein content, and a 3.5% increase in milk urea nitrogen content (6.75 mL/dL). In conclusion, probiotic microcapsules can effectively regulate intestinal flora, improving milk production, and quality in dairy cows.

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Section: Introductionmentioning

confidence: 99%

Compound probiotics microcapsules improve milk yield and milk quality of dairy cows by regulating intestinal flora

Wu,

Chang,

Zhang

et al. 2024

Food Bioengineering

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“…Enterococcus were significantly enriched in high‐FCR chickens and inhibited the abundance of pathogenic bacteria 33 . Exogenous probiotics have a subtle physiological effect on the host after colonization and dominant niche in the host intestine 34,35 . To investigate the accurate effects of the CPP supplementation, we performed a long‐term study in young layer chicks.…”

Section: Discussionmentioning

confidence: 99%

“…33 Exogenous probiotics have a subtle physiological effect on the host after colonization and dominant niche in the host intestine. 34,35 To investigate the accurate effects of the CPP supplementation, we performed a long-term study in young layer chicks. Similar to the results of these previous studies, we found that dietary supplementation with CPP significantly increased growth performance and reduced diarrhea, and that the effects of CPP supplementation were similar to those of CTC supplementation in layer chicks.…”

Section: Discussionmentioning

confidence: 99%

Dietary supplementation with probiotics increases growth performance, improves the intestinal mucosal barrier and activates the Wnt/β‐catenin pathway activity in chicks

Tong

Zeng

et al. 2023

J Sci Food Agric

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Background Probiotics comprise effective feed additives that can replace antibiotics in animal livestock production. However, mono‐strain probiotics appear less effective because of their instability. Therefore, the present study aimed to investigate dietary supplementation with compound probiotics (CPP) on growth performance, diarrhea rate and intestinal mucosal barrier, as well as the possible molecular mechanism, in chicks. In total, 360 1‐day‐old chicks of the Hy‐Line Brown Chicks were randomly divided into the control group (CON, basal diet), chlortetracycline group (500 mg kg−1 CTC) and compound probiotics group (1000 mg kg−1 CPP, consisting of Bacillus subtilis, Bacillus licheniformis, Enterococcus faecium and yeast). The experiment period was 56 days. Results The results showed that, in comparison with the CON group, CPP significantly increased the average daily feed intake and average daily gain of chicks and reduced diarrhea (P < 0.05). The probiotic group exhibited increased immune organ (i.e. spleen and thymus) mass and increased levels of serum immunoglobulin (Ig)A, IgM and IgG (P < 0.05) compared to the CTC group. In addition, the jejunal mass and morphology were improved in the probiotic group (P < 0.05). Moreover, CPP reinforced jejunal barrier function, as indicated by increased transepithelial electrical resistance, protein expression of occludin and claudin‐1, and diamine oxidase levels in the jejunum (P < 0.05). Likewise, enhanced fluorescence signals of proliferating cell nuclear antigen‐labeled mitotic cells and villin‐labeled absorptive cells in the jejunum (P < 0.05) suggested that CPP promoted intestinal stem cells activity. Mechanistically, the Wnt/β‐catenin signaling pathway, including β‐catenin, TCF4, c‐Myc, cyclin D1 and Lgr5, was amplified in the jejunum by CPP addition (P < 0.05). Conclusion The present study demonstrated that dietary supplementation with CPP reinforced the jejunal epithelial integrity by activating Wnt/β‐catenin signaling and enhanced immune function in chicks. © 2023 Society of Chemical Industry.

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“…Accumulating studies from various animal models in gut microbiota investigations, such as GF (complete absence of microbial exposure) animals, SPF (specific pathogen-free) animals, antibiotic-treated (broad-spectrum antibiotic cocktails) animals, and animals exposed to pathogenic bacterial infections, suggest that the gut microbiota plays an important role in the regulation of anxiety, mood, and cognition, indicating the possibility of using probiotics to modify the gut microbiota to control impulsive and compulsive behaviors in patients with neuropsychiatric disorders [ 158 , 159 , 160 , 161 ]. Like mammals, the gut microbiome plays a critical role in poultry health and welfare [ 119 , 162 , 163 ]. Laying hens showing high or low FP have different gut microbial populations [ 27 , 94 , 95 , 164 ] and metabolite profiles [ 96 , 97 ].…”

Section: Cecal Microbiota Transplantation Social Stress and Injurious...mentioning

confidence: 99%

The Impact of Early-Life Cecal Microbiota Transplantation on Social Stress and Injurious Behaviors in Egg-Laying Chickens

Fu,

Hu,

Zhang

et al. 2024

Microorganisms

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Injurious behaviors (i.e., aggressive pecking, feather pecking, and cannibalism) in laying hens are a critical issue facing the egg industry due to increased social stress and related health and welfare issues as well as economic losses. In humans, stress-induced dysbiosis increases gut permeability, releasing various neuroactive factors, causing neuroinflammation and related neuropsychiatric disorders via the microbiota–gut–brain axis, and consequently increasing the frequency and intensity of aggression and violent behaviors. Restoration of the imbalanced gut microbial composition has become a novel treatment strategy for mental illnesses, such as depression, anxiety, bipolar disorder, schizophrenia, impulsivity, and compulsivity. A similar function of modulating gut microbial composition following stress challenge may be present in egg-laying chickens. The avian cecum, as a multi-purpose organ, has the greatest bacterial biodiversity (bacterial diversity, richness, and species composition) along the gastrointestinal tract, with vitally important functions in maintaining physiological and behavioral homeostasis, especially during the periods of stress. To identify the effects of the gut microbiome on injurious behaviors in egg-laying chickens, we have designed and tested the effects of transferring cecal contents from two divergently selected inbred chicken lines on social stress and stress-related injurious behaviors in recipient chicks of a commercial layer strain. This article reports the outcomes from a multi-year study on the modification of gut microbiota composition to reduce injurious behaviors in egg-laying chickens. An important discovery of this corpus of experiments is that injurious behaviors in chickens can be reduced or inhibited through modifying the gut microbiota composition and brain serotonergic activities via the gut–brain axis, without donor-recipient genetic effects.

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Antioxidant Indexes and Immune Function of the Intestinal Flora of Compound Microecological Preparations

Cited by 11 publications

References 18 publications

Compound probiotics microcapsules improve milk yield and milk quality of dairy cows by regulating intestinal flora

Compound probiotics microcapsules improve milk yield and milk quality of dairy cows by regulating intestinal flora

Dietary supplementation with probiotics increases growth performance, improves the intestinal mucosal barrier and activates the Wnt/β‐catenin pathway activity in chicks

The Impact of Early-Life Cecal Microbiota Transplantation on Social Stress and Injurious Behaviors in Egg-Laying Chickens

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