Antioxidant peptides derived from the hydrolyzate of purple sea urchin (Strongylocentrotus nudus) gonad alleviate oxidative stress in Caenorhabditis elegans

Zhao, Sixu; Cheng, Qian; Peng, Qiong; Yu, Xuesong; Yin, Xiquan; Ming, Liang; Wah, Chung; Huang, Zebo; Jia, Weizhang

doi:10.1016/j.jff.2018.07.060

Cited by 49 publications

(49 citation statements)

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“…However, it is also observed that the antioxidant activity does not show in descending molecular weight order. This result is slightly different from previous reports that the lower molecular size fraction had the higher antioxidant activities [19,[26][27][28], but similar to the result reported by Wong et al [27]. It could be conjectured that the peptides with lower molecular weight (HPH-3, <3 kDa) could react with free radicals much easier and convert them to more stable products and then terminate the radical chain reactions [29], which might make it clear that HPH-3 shows the best antioxidant activity.…”

Section: Purification Of Antioxidative Peptidescontrasting

confidence: 99%

“…The DPPH radical scavenging activity of purified fractions was evaluated according to the published method with minor modifications [19]. Briefly, the sample group and blank group comprised 50 µL of the sample solution or vehicle mixed with 50 µL of 0.4 mM DPPH; and the control group comprised 50 µL of sample solution and 50 µL of ethanol (the vehicle of DPPH radical).…”

Section: Radical Scavenging Activity Assaymentioning

confidence: 99%

“…Housefly (Musca domestica L.) is one of the best-known economic insects with many features like strong reproductive capacity, high adaptability, saprophagous peculiarity and pathogenic resistance ability. Housefly larvae and pupae, namely "wuguchong" in traditional Chinese medicine, are rich in biomass with high-quality proteins, polyunsaturated fats, chitosan, vitamins, minerals and other nutrients [19]. It has been used for more than hundreds of years in clinical practice to treat gastrointestinal disease and heal wounds like malnutritional stagnation, damp-heat diarrhea, vomiting, decubital necrosis, ecthyma and lip boils [20,21].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Housefly Pupae-Derived Antioxidant Peptides Exerting Neuroprotective Effects on Hydrogen Peroxide-Induced Oxidative Damage in PC12 Cells

Sun

Zhang

Yang³

et al. 2019

Molecules

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In this study, two antioxidant peptides were identified and characterized from the alcalase-hydrolysate of housefly (Musca domestica L.) pupae guided by ABTS cation radical scavenging activity. Peptides sequences were identified as DFTPVCTTELGR (DR12, 1338.48 Da) and ARFEELCSDLFR (AR12, 1485.66 Da) using nano-liquid chromatography-tandem mass spectrometry (LC-MS/MS). Both DR12 and AR12 exert strong ABTS cation radical scavenging ability with EC50 values of 0.39 and 0.35 mM, respectively. Moreover, AR12 can effectively protect PC12 cells from oxidative damage induced by hydrogen peroxide (H2O2) by decreasing intracellular reactive oxygen species (ROS) and malonaldehyde (MDA), recovering cellular mitochondrial membrane potential (MMP), and increasing the activity of intracellular superoxide dismutase (SOD). Stability tests suggest that AR12 is competent for the challenge of heating, acid, alkali or simulated gastrointestinal (GI) digestion and exhibits great activity to remove ABTS cation radical. DR12 shows a great stability against heating, but its antioxidative ability declines after being treated with acid, alkali or simulated GI digestion. In general, both DR12 and AR12 identified from housefly pupae hydrolysate stand a chance of being potential antioxidants or precursors to antioxidants and AR12 might be applied in the field of neuroprotection.

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Section: Purification Of Antioxidative Peptidescontrasting

confidence: 99%

Section: Radical Scavenging Activity Assaymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Housefly Pupae-Derived Antioxidant Peptides Exerting Neuroprotective Effects on Hydrogen Peroxide-Induced Oxidative Damage in PC12 Cells

Sun

Zhang

Yang³

et al. 2019

Molecules

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“…Total RNA was extracted from approximately 1000 worms using Trizol reagent. The quality and quantity of RNA was assessed with Nucleic acid/Protein analyzer (DU730, Beckman Coulter, Inc., Fullerton, California, USA) and gel electrophoresis [44]. Complementary DNA (cDNA) was obtained using the PrimeScript™ RT Reagent Kit (Takara Biotechnology, Dalian, China) under the following conditions: 37 • C for 15 min, and then to 85 • C for 5 s. A Bio-Rad MiniOption™ Real Time PCR Detection System (Bio-Rad, Hercules, CA, USA) was used to analyze quantitative real-time PCR with SYBR Green Super-mix (Takara Biotechnology, Dalian, China).…”

Section: Real-time Rt-pcrmentioning

confidence: 99%

Effects of Orange Extracts on Longevity, Healthspan, and Stress Resistance in Caenorhabditis elegans

et al. 2020

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Orange, with various bioactive phytochemicals, exerts various beneficial health effects, including anti-cancer, antioxidant, and anti-inflammatory properties. However, its anti-aging effects remain unclear. In this study, the Caenorhabditis elegans (C. elegans) model was used to evaluate the effects of orange extracts on lifespan and stress resistance. The results indicated that orange extracts dose-dependently increased the mean lifespan of C. elegans by 10.5%, 18.0%, and 26.2% at the concentrations of 100, 200, and 400 mg/mL, respectively. Meanwhile, orange extracts promoted the healthspan by improving motility, and decreasing the accumulation of age pigment and intracellular reactive oxygen species (ROS) levels without damaging fertility. The survival rates of orange extract-fed worms were obviously higher than those of untreated worms against thermal and ultraviolet-B (UV-B) stress. Moreover, the activities of superoxide dismutase (SOD) and catalase (CAT) were significantly enhanced while malondialdehyde (MDA) contents were diminished. Further investigation revealed that worms supplemented with orange extracts resulted in upregulated levels of genes, including daf-16, sod-3, gst-4, sek-1, and skn-1, and the downregulation of age-1 expression. These findings revealed that orange extracts have potential anti-aging effects through extending the lifespan, enhancing stress resistance, and promoting the healthspan.

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“…Sea urchins are invertebrates belonging to the class Echinoidea (phylum Echinodermata), which incorporates more than 700 described extant species worldwide. Recently, they have been the object of growing interest as available sources of new biomolecules which, on the basis of their activity as antibacterial, antiviral, antiprotozoal, antifungal, and anticancer agents, may be potential candidates for drug discovery and development [1][2][3][4]. Indeed, these animals have developed complex molecular mechanisms to use in various biological processes, such as communication, defense, reproduction, and antimicrobial resistance.…”

Section: Introductionmentioning

confidence: 99%

Cell-Free Coelomic Fluid Extracts of the Sea Urchin Arbacia lixula Impair Mitochondrial Potential and Cell Cycle Distribution and Stimulate Reactive Oxygen Species Production and Autophagic Activity in Triple-Negative MDA-MB231 Breast Cancer Cells

Luparello

Ragona

Asaro

et al. 2020

JMSE

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Triple-negative breast cancer (TNBC) is a highly malignant tumor histotype which lacks effective targeted therapies, thereby being considered as the most aggressive form of breast carcinoma. To identify novel compounds which could counteract TNBC cell growth, we explored the in vitro effects of crude extracts and <10 kDa-filtered fractions of the coelomic fluid obtained from the sea urchin Arbacia lixula on TNBC MDA-MB231 cells. We examined cell viability, cycle distribution, apoptotic/autophagic activity, and mitochondrial polarization/cell redox status. Here, we report the first data demonstrating an anti-TNBC effect by A. lixula-derived coelomic fluid extracts. Thus, identification of the water-soluble bioactive component(s) contained in the extracts deserve(s) further investigation aimed to devise novel promising prevention and/or treatment agents effective against highly malignant breast tumors.Further studies conducted by Björn et al. [8] demonstrated that the synthetic peptides sequentially derived from the previously characterized centrocin 1 (CEN1 HC-Br and CEN1 HC) showed prominent microbicidal and anti-inflammatory activities on in vivo and in vitro mammalian models, representing an interesting resource for the treatment of infections in humans.Furthermore, a recent study by Katelnikova et al. [9] highlighted that a glycopeptide fraction (GPF) derived from internal organs of the green sea urchins Strongylocentrotus droebachiensis possesses relevant anti-inflammatory effects, especially for the treatment of bronchitis. Moreover, the absorption and pharmacokinetics of GPF following single and repeated intranasal administration were evaluated over the course of seven days in rats [10].Another echinoderm species from which bioactive molecules were studied is Arbacia lixula, commonly found in the Mediterranean Sea and in the African Atlantic coast [11]. So far, the biomedical applications of molecules extracted from this marine invertebrate are very limited. Of note, Stabili et al. [12] demonstrated the powerful antioxidant effect exerted by the lysate of A. lixula's coelomocytes, the immune cells present in the coelomic fluid (CF) of the sea urchin, whereas Cirino et al. [13] highlighted that A. lixula's eggs are a rich source of astaxanthin, a radical scavenger that can prevent neurodegenerative diseases. These promising findings prompted an extension of the study on the pharmacological potential of A. lixula-derived substances, whose massive sourcing represents an easy task due to the abundance of this marine species in waters surrounding Sicily, also being a thermophilic species which is expanding very quickly due to the increase of the surface temperature of the Mediterranean Sea [14].Triple-negative breast cancers (TNBC) are categorized among the highly "aggressive" malignant carcinomas. In fact, the lack of expression of estrogen, progesterone, and epidermal growth factor type 2 (HER2) receptors makes them poorly responsive to hormonal therapies and to HER2-targeting drugs, and the TNBC histotype ...

show abstract

Antioxidant peptides derived from the hydrolyzate of purple sea urchin (Strongylocentrotus nudus) gonad alleviate oxidative stress in Caenorhabditis elegans

Cited by 49 publications

References 50 publications

Housefly Pupae-Derived Antioxidant Peptides Exerting Neuroprotective Effects on Hydrogen Peroxide-Induced Oxidative Damage in PC12 Cells

Housefly Pupae-Derived Antioxidant Peptides Exerting Neuroprotective Effects on Hydrogen Peroxide-Induced Oxidative Damage in PC12 Cells

Effects of Orange Extracts on Longevity, Healthspan, and Stress Resistance in Caenorhabditis elegans

Cell-Free Coelomic Fluid Extracts of the Sea Urchin Arbacia lixula Impair Mitochondrial Potential and Cell Cycle Distribution and Stimulate Reactive Oxygen Species Production and Autophagic Activity in Triple-Negative MDA-MB231 Breast Cancer Cells

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