Antioxidant, Phytochemical and Antibacterial Action of Himalayan Medicinal Herbs Peristrophe Bicalyculata Leaves Extract Against Respiratory Tract Pathogens

Abstract

Objective: Evaluate the antioxidant, phytochemicals and antibacterial screening of leaves extracts of Peristrophe bicalyculata.Methods: The antibacterial activity against the respiratory tract pathogens i.e., Staphylococcus aureus (MTCC 1144), Streptococcus pneumoniae (MTCC 655), Streptococcus pyogenes (MTCC 442), Pseudomonas aeruginosa (MTCC 2474) and Klebsiella pneumoniae (MTCC 4030) was examined by the method agar well diffusion and the minimum inhibitory concentration (MICs) was determined by the method of twofold serial dilution. Broad spectrum antibiotic erythromycin was used as positive control and dimethyl sulphoxide (DMSO) used as negative control. The qualitative method was adapted for phytoconstituents screening and antioxidant activity of plant extract was examined by DPPH free radical scavenging method.Results: The results showed that the chloroform (CHF) extract has a higher degree of antibacterial potency then the other extract. The zone of inhibition showed by chloroform extract against tested bacteria ranged between 9.3±0.59 mm to 26.6±0.66 mm, respectively. MICs values were recorded between 6.25 mg/ml to 25 mg/ml against all the test organisms. Phytoconstituents analysis of P. bicalyculata extract exposed the presence of alkaloids, flavonoids, glycosides, steroids, saponins and tannins. The methanolic extract of P. bicalyculata % inhibition of DPPH radical is up to 86.33%. The P. bicalyculata (Methanolic extract) gives best antioxidant activity than another extract.Conclusion: This investigation ropes a good answer to the use of P. bicalyculata as a natural antioxidant and in herbal medicine as a support for the development of new drugs and phytomedicine in the foundation for its use in remedial of respiratory infectious diseases.

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Chaksini (Peristrophe Bicalyculata (Retz) Nees.: A Comprehensive Review on a Lesser-Known Herb of Unani Medicine

“…Another study conducted by Giwa OE et al found that ethanolic extract of Peristrophe bicalyculata leaves inhibited growth of Staphylococcus aureus, Klebsiella spp., Pseudomonas aeruginosa, Aspergillus niger, Aspergillus clavatus and [38] Rhizopus stolonifer . Chloroform extract of Peristrophe bicalyculata also demonstrated and was found significant anti-bacterial property against Staphylococcus aureus (MTCC 1144) (26±0.66 mm) and lowest inhibition against K. pneumonia (MTCC 4030)(18.3±0.79 mm) [39] Antioxidant activity A study conducted by Arya P (2018) showed that methanolic leaves extract of Peristrophe bicalyculata has potent DPPH free radical scavenging ability in comparison to other [39] extracts . Victor DS et al performed a comparative in vitro study regarding antioxidant properties between Ocimum gratissimum, Vitex doniana, Carica papaya and Peristrophe bicalyculata using DPHH free radical scavenging activity.…”