Antioxidative and superoxide scavenging activities of retrochalcones in Glycyrrhiza inflata

Haraguchi, Hiroyuki; Ishikawa, Hideyuki; Mizutani, Kenji; Tamura, Yukiyoshi; Kinoshita, Takeshi

doi:10.1016/s0968-0896(97)10034-7

Cited by 226 publications

(153 citation statements)

References 44 publications

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“…Lipid peroxidation is initiated by active oxygen species attacking unsaturated fatty acids and is propagated by a chain reaction cycle involving lipids, peroxy radicals, and lipid hydroperoxides. 25) Peroxidation of the cell membrane phospholipids and accumulation of lipid peroxides are expected to alter the membrane fluidity and permeability, consequently leading to disruption of membrane structure and function. The results of the present study suggest that panduratin A is capable of ameliorating hepatocyte lipid peroxidation caused by t-BHP.…”

Section: Discussionmentioning

confidence: 99%

Protective Effects of Panduratin A against Oxidative Damage of <i>tert</i>-Butylhydroperoxide in Human HepG2 Cells

Sohn

Han

Lee

et al. 2005

Biological & Pharmaceutical Bulletin

106

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Reactive oxygen species (ROS) act as subcellular messengers in such complex cellular processes as mitogenic signal transduction, gene expression, and regulation of cell proliferation.1) Excessive production of ROS may, however, cause oxidative stress, loss of cell function, and ultimately apoptosis or necrosis.2) Fortunately, cells possess antioxidant molecules which can neutralize ROS before they induce damage on vital components. However, oxidative stress occurs when antioxidant systems are overwhelmed by ROS. Oxidative stress is considered to play a prominent role in the causation of many diseases including aging, cardiovascular disease, cancer and liver injury. 3,4) tert-Butylhydroperoxide (t-BHP) is an organic hydroperoxidant that can be metabolized to free radical intermediates, which can subsequently initiate lipid peroxidation, affect cell integrity, and form covalent bonds with cellular molecules resulting in cell injury. 5) t-BHP causes leakage of lactate dehydrogenase (LDH) and formation of malondialdehyde (MDA) in hepatocytes, 6) and also mediates DNA base damage in mammalian cells. 7) These phenomena are similar to the oxidative stress occurring in the cell and/or tissue.Because of its unique metabolism and relationship to the gastrointestinal tract, the liver is an important target of the toxicity of drugs, xenobiotics, and oxidative stress. 8) Reactive oxygen species derived from chemicals or drugs exposed to liver cells seem to mediate liver injury, although the mechanisms of free radical toxicity are not well understood. 9)Therefore, primary importance is to understand the role of antioxidants which can reduce the oxidative stress induced by reactive intermediates from various chemicals and drugs. We have investigated hepatoprotective drugs, based on the measurement of cytotoxicity of t-BHP against human-liver-derived HepG2 cells. HepG2 cells have been suggested to be a useful in vitro model for investigation of the metabolism and toxicity of drugs compared with animal cells, since HepG2 cells retain many of the specialized functions which are characteristics of normal human hepatocytes, including the synthesis and secretion of plasma proteins. 10)Kaempferia pandurata ROXB. is a perennial herb of the family Zingiberaceae cultivated in some tropical countries, including Thailand and Indonesia. Its rhizome has been used as a food ingredient, an aphrodisiac, and a folk medicine for the treatment of colic disorder, fungal infections, dry cough, rheumatism, and muscular pains.11,12) Panduratin A (Fig. 1A) is a chalcone derivative isolated from K. pandurata. It was recently reported that panduratin A possessed significant topical anti-inflammatory activity in murine macrophages, 12) and in the TPA-induced ear edema in rats. 13)The objective of the current study was to examine the ability of panduratin A to protect hepatocytic injury induced by t-BHP and to characterize the mechanisms involved. For this purpose, we have studied the cell viability, lipid peroxidation, glutathione (GSH) level, and ROS le...

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Section: Discussionmentioning

confidence: 99%

Protective Effects of Panduratin A against Oxidative Damage of <i>tert</i>-Butylhydroperoxide in Human HepG2 Cells

Sohn

Han

Lee

et al. 2005

Biological & Pharmaceutical Bulletin

106

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“…They also displayed antioxidative and superoxide scavenging effects. 12) This paper describes the cytotoxicity-guided isolation and structure elucidation of a new retrochalcone 4.…”

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confidence: 99%

Cytotoxic Allyl Retrochalcone from the Roots of Glycyrrhiza inflata

2005

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“…Licochalcones are known to be reactive oxygen scavengers. 20) In regard to the regulation of Bcl-2, NF-kB binding activity was considerably retained as compared with the loss of Bcl-2 expression in the same cells (15 mM Lico-E treatment) suggesting that Bcl-2 expression may be regulated by other transcription factors besides NF-kB.…”

Section: Discussionmentioning

confidence: 88%

Induction of Apoptosis by the Licochalcone E in Endothelial Cells via Modulation of NF-.KAPPA.B and Bcl-2 Family

Chang

Yoon

Park

et al. 2007

Biological & Pharmaceutical Bulletin

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Antioxidative and superoxide scavenging activities of retrochalcones in Glycyrrhiza inflata

Cited by 226 publications

References 44 publications

Protective Effects of Panduratin A against Oxidative Damage of <i>tert</i>-Butylhydroperoxide in Human HepG2 Cells

Protective Effects of Panduratin A against Oxidative Damage of <i>tert</i>-Butylhydroperoxide in Human HepG2 Cells

Cytotoxic Allyl Retrochalcone from the Roots of Glycyrrhiza inflata

Induction of Apoptosis by the Licochalcone E in Endothelial Cells via Modulation of NF-.KAPPA.B and Bcl-2 Family

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