Antioxidative, Antimicrobial Activity and HPLC Analysis of Ornithogalum pyrenaicum

Abstract

Introduction: The aim of this study was to specify the antioxidant, antimicrobial activity and phenolic contents of Ornithogalum pyrenaicum. Methods: The antioxidant capacity of O. pyrenaicum was determined by 2,2-diphenylpicrylhydrazyl (DPPH) method and ferric reduction antioxidant power by FRAP method. The total phenolic content (TPC) was of the samples was determined using spectrophotometric method. The phenolic contents the samples were analyzed by reverse phase-high performance liquid chromatography (RP-HPLC). Antimicrobial activity was researched on 9 microorganism by agar diffusion method. Results: As a result of the study, high phenolic contents and strong antioxidant capacitiy were observed. Phenolic compounds were detected as p-coumaric acid and benzoic acid. Additionally, it was determined that O. pyrenacium had considerable antimicrobial activity on Yersinia pseudotuberculosis, Staphylococcus aureus and moderate activity on Pseudomonas aeruginosa and Mycobacterium smegmatis. Conclusion: In conclusion, O. pyrenaicum extract could be evaluated in the pharmaceutical and cosmetic fields due to their antioxidant and antimicrobial potential and phenolic compounds.