Antioxidative effect of compounds isolated from Globularia alypum L. structure–activity relationship

Es‐Safi, Nour‐Eddine; Kollmann, Albert; Khlifi, Samira; Ducrot, Paul‐Henri

doi:10.1016/j.lwt.2006.08.019

Cited by 64 publications

(59 citation statements)

References 33 publications

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“…These extracts were dryly evaporated and then inserted into the methanol in order to have concentrations of 5mg/ml for each one. The free radical activity of these extracts was measured according to the method described by Es-Safi et al (2007): 25μl of the extract was tested with 2.5 ml of a methanolic solution of DPPH (0,004%). The optical density C was measured by the SHIMADZU spectrophotometer to 517 nm, after 30 minutes of incubation in ambient air temperature room in the darkness.…”

Section: Biological Activitiesmentioning

confidence: 99%

Untitled

2017

Int. J. Curr. Res. Biosci. Plant Biol.

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A b s t r a c t A r t i c l e I n f oThis study is to determine the interest of the people of the rural region Deba-Relizane (West Algeria) for the phytotherapy by using the medicinal plant Thymus vulgaris called zàatar. The density of this plant is around 3-8 plts/ 200 m². An investigation has been conducted on the users of this plant through precise questions about the use of this plant, included a 560 users of the area population. Chemical and biological tests were conducted on the content of this plant about active substances, and determine their effectiveness. The (100g) is put in an the extraction process by maceration a 100g of material vegetal in the ethanol mixture/water (30/70: v/v) during 72 hrs, confrontations with increasing polarity solvents, with ether of oil and ethyl acetate AE, methyl ethyl ketone, MEK. The mobile phase is a mixture of organic solvents which gives the best separations, and revealing with the UV (254Nm, and 365Nm). Thymus vulgaris has 8.97± 0,002 Mg EAT/g; The flavonoids quantity of the ethanolic extracts as quercetin equivalent (EQ) is 8.56 ±0.001 mg/g. Chromatographic behavior of EA and MEK phase of Thymus vulgaris on polyamide plate (DC6), in the solvent (toluene /methanol/ethanol) (3/4/3) and in the solvent (acetic acid /methanol/ water/) (95/5/5). The absorption peaks in the area UV-Screw: BI (329.5 Nm) and BII (280.5 Nm), make us think that this substance belongs to flavonols, all the previous criteria, the molecule like following: keampférol 3OR', 7OR", lutéoline 7OR', quercetin 3OR ', 7OR", chrysin 7OR', apigénine 5OR', 7OR". The biological study was carried out through the effect, free radical scavenger action of the active substances isolated from Thymus vulgaris. The free radical scavenger activity of our products was evaluated by the method of DPPH, (2-2-diphényl-1-picrylhydrazyl), The results of the antibacterial action of the substances isolated from Thymus vulgaris the diameters of the inhibition zones reveal fairly important inhibition zones with respect to Staphylococcus aureus whom diameter of inhibition zones vary from 8-18 mm for Escherichia coli, and of 10-15 mm for Staphylococcus aureus. It is concluded from the present study that the users of Thymus vulgaris are lacking scientific information, but the laboratory research proved the presence of effective and influential substances on various harmful organisms, which can be evaluated in several medical and biological fields.

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Section: Biological Activitiesmentioning

confidence: 99%

Untitled

2017

Int. J. Curr. Res. Biosci. Plant Biol.

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“…One can use several methods for antioxidant capacity determination such as Trolox equivalent antioxidant capacity (TEAC) using usually 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical [13,14], ferric reducing antioxidant power (FRAP) using ferric complex reduction [15] or oxygen radical absorbance capacity (ORAC) [16]. The next feasibility is the method using reduction of the free 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical [17]. This method is commonly used for the reference determination of antioxidant capacity and is pretty easy to perform.…”

Section: Hplc/uv System and Conditionsmentioning

confidence: 99%

Comparison of pressurised fluid and ultrasonic extraction methods for analysis of plant antioxidants and their antioxidant capacity

et al. 2010

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Abstract:The analytical method based on the high-per formance liquid chromatography coupled with UV detection (HPLC/ UV) for determination of selected antioxidants (i.e., esculetin, scopoletin, 7-hydroxycoumarine, r utin, xanthotoxin, 5-methoxypsoralen and quercetin) in plant material was developed. Pressurised fluid extraction (PFE) and ultrasonic extraction (USE) methods for the isolation of these compounds from ten real plant samples were used. Both extraction methods were optimised and compared to each other. For the proposed HPLC/UV method the LOQ values (limit of quantification) in the range from 22.7 (xanthotoxin) to 97.2 ng mL -1 (r utin) were obtained. For all extracts the antioxidant capacity based on the reduction of free 2,2-diphenyl-1-picr ylhydrazyl radical (DPPH) was also determined. Results ranged from 82.04 to 94.43% of DPPH radical inhibition for PFE method and from 76.01 to 89.94% in the case of USE method.

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“…Phenylethanoid glycosides possess variable antioxidant properties, such as verbascoside which is reported to exhibit antimicrobial, anti-inflammatory, immunosuppressive, antitumour, diuretic and antibacterial activities [26]. Moreover, both these structural isomers are known to exhibit analgesic properties [27]. Many analysis methods have been developed to study various kinds of phenylethanoid glycosides compounds in different medicine plants [28][29].…”

Section: Introductionmentioning

confidence: 99%

Separation and analysis of phenylethanoid glycosides inLamiophlomis rotataby high-performance liquid chromatography

et al. 2013

Acta Chromatographica

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Summary.A simple hydrolysis method has been developed for determination of phenylethanoid glycosides in Lamiophlomis rotata (L.R.). Different kinds of phenylethanoid glycosides were hydrolyzed in hydrochloric acid solution to produce corresponding phenethyl alcohols and cinnamic acids, mainly containing hydroxytyrosol, homovanillyl alcohol, 3,4-dimethoxyphenethyl alcohol, caffeic acid, fumalic acid and 3,4-dimethoxycinnamic acid. The six analytes could be determined simultaneously by high-performance liquid chromatography (HPLC). The effects of mobile phase, pH and concentration of running buffer, detection wavelength, flow rate and injection volume were also investigated. Under the optimum conditions, the six hydrolyzates could be perfectly separated within 45 min. The response was linear over four orders of magnitude with detection limits (S/N ＝ 3) ranging from 1 × 10 −8 to 1.5 × 10 −4 mol L −1 for the analytes. The method has been successfully applied to the analysis of real sample Du-Yi-Wei capsule and Qi-Zheng-Yan-Tong patch, with satisfactory results.

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Antioxidative effect of compounds isolated from Globularia alypum L. structure–activity relationship

Cited by 64 publications

References 33 publications

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Comparison of pressurised fluid and ultrasonic extraction methods for analysis of plant antioxidants and their antioxidant capacity

Separation and analysis of phenylethanoid glycosides inLamiophlomis rotataby high-performance liquid chromatography

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