Antioxidative Potentials of Cooked Bambara Groundnut Based Diet (Vigna Subterranean) on Lipid Peroxidation Status in Pretreated Alloxan Induced Diabetic Rats

Abstract

The present study examines the antioxidant potentials of Cooked Bambara groundnut (Vigna subterranean) based diet in pretreated alloxan induced diabetic rats. Thirty (30) male albino rats (Rattus novergicus) were ramdomly divided into three experimental groups (A, B, C) i.e 10 rats in each group. GroupA received standard formulated diet, GroupB were fed with 50:50 ratio of corn starch and cooked Bambara nut based diet, GroupC received whole cooked Bambara nut based diet for four weeks. After four weeks of feeding, the three groups were sub-divided into subgroups A 1 , A 2 , B 1 , B 2 and C 1 , C 2 .Subgroups A 2 , B 2 and C 2 were induced with 150mg/kg body weight of alloxan by a single intraperitonial injection. Each Subgroups were maintained on their respective diet for two weeks, after which the blood samples were collected via cardiac puncture, heart was removed and assayed for Malondialdehyde (MDA), Reduced glutathione (GSH) and antioxidant enzyme activities (Catalase, Superoxide dismutase). Significant (p < 0.05) elevation in MDA, and a reduction in antioxidant activities were observed in plasma and heart of groupA 2 compared with groupA 1 . A significant decrease in plasma and heart MDA level was observed in groupC 2 compared with groupC 1 .Plasma and heart antioxidant activities of groupC 2 significantly increased (P ≤ 0.05) when compared with groupC 1 . There was no significant difference in plasma and heart MDA level and antioxidant activities in groupB 2 compared with groupB 1 . In conclusion, Cooked Bambara groundnut exhibits its antioxidant potentials by alleviating oxidative stress.However, it appears that increased concentration of cooked Bambara groundnut in the diet

Evaluation of phytochemical constituents, proximate contents and glycemic index of bambara groundnut (Vigna subterranea L. Verdc) varieties grown in Northeastern Nigeria

“…The formulated feed containing seed flour of Bambara groundnut varieties composed seed flour (56%), cray-fish (20%), vegetable oil (5%), rice bran (4%), sucrose (10%), and vitamin/mineral mixture (5%), respectively while the basal feed composed of the same ingredients as the experimental feeds except for Bambara groundnut seed flour was replaced with corn-flour (56%) as described by Olubunmi et al (2017). The Bambara groundnut seed varieties formulated feeds containing seed flour of Bambara groundnut were tagged as, ALK/01, ALK/02, ALK/03 and ALK/04 feed.…”

Effects of Consuming Different Varieties of Bambara Nut (Vigna subterranea) Seeds on Liver and Kidney of Diabetic and Non-diabetic Subject

“…The formulated feed containing flour of Bambara nuts seeds' varieties composed of the following: Bambara nut seed flour (56%), Cray-fish (20%), vegetable oil (5%), rice bran (4%), sucrose (10%), Vitamin/mineral mixture (5%), respectively. The control feed used was animal's feed (Grower's mash) which represented a wellbalanced diet consisting of significant percentage of carbohydrate, protein, fats (lipid), vitamins and minerals while the basal feed composed of same ingredients as the experimental feeds except that Bambara nut seed flour was replaced with cornflour (56%) as prepared by Olubunmi et al [25]. The Bambara nut seeds' varieties formulated feeds were tag as; ALK01 feed, ALK02 feed, ALK03 feed and ALK04 feed.…”

Effect of Consuming Different Varieties of Bambara Groundnut (Vigna subterranea) Seeds on Glycaemia and Lipid Profile of Diabetic and Non-Diabetic Rats

“…The formulated feed containing flour of Bambara groundnut seed varieties composed of the following: Bambara groundnut seed flour (56%), Cray-fish (20%), vegetable oil (5%), rice bran (4%), sucrose (10%), Vitamin/mineral mixture (5%), respectively. The control feed used was animal feed (Grower's mash) which represented a wellbalanced diet consisting of significant percentage of carbohydrate, protein, fats (lipid), vitamins and minerals while the basal feed composed of same ingredients as the experimental feeds except that Bambara groundnut seed flour was replaced with corn-flour (56%) as prepared by Olubunmi et al 19 . The Bambara groundnut seed varieties formulated feeds were tag as; ALK01 feed, ALK02 feed, ALK03 feed and ALK04 feed.…”