Antioxidative status, immunological responses, and heat shock protein expression in hepatopancreas of Chinese mitten crab, Eriocheir sinensis under the exposure of glyphosate

Hong, Yuhang; Huang, Yi; Yan, Guangwen; Pan, Chao; Zhang, Jilei

doi:10.1016/j.fsi.2018.12.020

Cited by 63 publications

(25 citation statements)

References 41 publications

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“…Under the long‐term stress, the continuous decrease of enzyme activity maybe since the accumulation of two insecticides in the crab has exceeded the tolerance of crabs. Changes of SOD and CAT activity were similar to those reported by Hong et al （Hong, Huang, Yan, Pan & Zhang, 2019), which was induced under low concentration of deltamethrin treatment, and the activity was inhibited with time, this is due to the increase in antioxidants caused by oxidative damage of organisms exposed to low concentrations; while MDA activity was not altered by the presence of deltamethrin （Hong, Huang, Yan, & Huang, 2019) . However, they found no significant change in enzyme activity, possibly because of the duration of exposure, the longest duration of which was 96 hours, while exposure in the current study was 90 days.…”

Section: Discussionsupporting

confidence: 85%

“…Histopathology in this work demonstrated that the two insecticides were respectively damaged the hepatopancreas of E. sinensis . The hepatopancreas is an extremely important organ to the crabs, which can secrete a variety of enzymes, participating in the growth, development, moulting, material metabolism, nutrient metabolism and other life activities (Hong, Huang, Yan, Pan & Zhang, 2019; Wang et al, 2016). The hepatopancreas became deformation, enlargement and had many vacuoles after exposure.…”

Section: Discussionmentioning

confidence: 99%

“…The Chinese mitten crab Eriocheir sinensis is regarded as a high market‐valuable freshwater species for China's cultivation for its rapid growth, high fecundity and nutrition richness (Hong, Huang, Yan, Pan, & Zhang, 2019). Over the past decades, intensive crab cultivation industry has undergone rapid development, while inevitably been limited by the great economic loss suffering from various diseases due to high stocking density (Wang et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

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Hepatopancreatic necrosis disease (HPND) in Chinese mitten crab Eriocheir sinensis tightly linked to low concentration of two insecticides

Shen

Zhang

et al. 2020

Aquaculture Research

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To date, hepatopancreatic necrosis disease (HPND) in Eriocheir sinensis is causing huge financial losses to the aquaculture industry while the cause of HPND remains unclear. Our previous study has implied that HPND is probably relevant to the damages caused by insecticide residues. In this work, the comparative studies regarding histopathology and trends in immune‐related/detoxifying enzymes activities of the crabs were performed between exposed to the low concentration of insecticides (i.e. β‐cypermethrin 8.52 × 10−12 g/L and trichlorfon 5 × 10−5 g/L) in a long‐term (90‐days) and suffered from HPND. The concentration was obtained by a median‐lethal concentration (LC50) and a safe concentration (SC), and it was 10 times lower than the safe concentration in this experiment. This work's principal aim was to investigate the potential chronic effects of low concentrations of insecticides exposure on crabs and their relevance with HPND. As a result, crabs exposed to the low concentration of insecticides caused similar clinical symptoms and severity in hepatopancreas and other tissue damages like HPND. Meanwhile, catalase (CAT), superoxide dismutase (SOD), malondialdehyde (MDA), acid phosphatase (ACP), alkaline phosphatase (AKP), lysozyme (LZM) and carboxylesterase (CarE) enzymatic activity increased after exposure to the two insecticides and then decreased in the later stages, while the activity of glutathione S‐transferase (GSTs) has barely changed. Results of enzymatic activity indicated that the two insecticides induced oxidative damage to the hepatopancreas of E. sinensis. These evidence indicated that the chronic damage caused by the low concentration of insecticides, which is considered insecticide residue, might be part of the causes of HPND in crabs. The present work may enhance our understanding of the pathogenesis of HPND and provide a theoretical basis for the management of intensive crab aquaculture.

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Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Hepatopancreatic necrosis disease (HPND) in Chinese mitten crab Eriocheir sinensis tightly linked to low concentration of two insecticides

Shen

Zhang

et al. 2020

Aquaculture Research

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“…Our previous research evaluating the transcriptome, proteome and metabolome of P. plecoglossicida incubated under virulent (18°C) and avirulent (28°C) temperatures confirmed that P. plecoglossicida was a temperature-dependent facultative pathogen, and showed that htpG was significantly highly expressed under 18°C (16, 17). htpG encodes the protein HtpG, a member of heat-shock proteins (HSPs) family (18, 19), which was first described in Escherichia coli (20). Null mutation of this gene is not lethal to bacterial cells and only results in impaired growth at high temperatures (21).…”

Section: Introductionmentioning

confidence: 99%

Dual RNA-Seq Unveils Pseudomonas plecoglossicida htpG Gene Functions During Host-Pathogen Interactions With Epinephelus coioides

et al. 2019

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Pseudomonas plecoglossicida is a temperature-dependent opportunistic pathogen which is associated with a variety of diseases in fish. During the development of “white nodules” disease, the expression of htpG in P. plecoglossicida was found to be significantly up-regulated at its virulent temperature of 18°C. The infection of htpG -RNAi strain resulted in the onset time delay, reduction in mortality and infection symptoms in spleen of Epinephelus coioides , and affected the bacterial tissue colonization. In order to reveal the effect of htpG silencing of P. plecoglossicida on the virulence regulation in P. plecoglossicida and immune response in E. coioides , dual RNA-seq was performed and a pathogen-host integration network was constructed. Our results showed that infection induced the expression of host genes related to immune response, but attenuated the expression of bacterial virulence genes. Novel integration was found between host immune genes and bacterial virulence genes, while IL6, IL1R2, IL1B , and TLR5 played key roles in the network. Further analysis with GeneMANIA indicated that flgD and rplF might play key roles during the htpG -dependent virulence regulation, which was in accordance with the reduced biofilm production, motility and virulence in htpG -RNAi strain. Meanwhile, IL6 and IL1B were found to play key roles during the defense against P. plecoglossicida , while CELA2, TRY, CPA1, CPA2 , and CPB1 were important targets for P. plecoglossicida attacking to the host.

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“…For this reason, organisms have evolved a set of antioxidant defense mechanisms to prevent oxidative stress damage caused by excessive ROS. Superoxide dismutase (SOD) and catalase (CAT) are considered to be important enzymes for removing ROS in crustaceans (Liu et al, 2018; Hong et al, 2019). Therefore, it is important to study the effects of air exposure stress on both respiratory metabolism and antioxidant responses in crustaceans to understand their full physiological responses to hypoxia stress.…”

Section: Introductionmentioning

confidence: 99%

Respiratory Metabolism and Antioxidant Response in Chinese Mitten Crab Eriocheir sinensis During Air Exposure and Subsequent Reimmersion

Bao

Xing

et al. 2019

Front. Physiol.

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Chinese mitten crab, Eriocheir sinensis , often suffers from severe air exposure stress during transportation and culture; high mortality occurs due to desiccation. In this study, the effects of air exposure stress (0, 2, 4, 8, and 16 h) and reimmersion (2, 6, 12 h) on respiratory metabolism and antioxidant responses in Chinese mitten crabs were studied under laboratory conditions. The results showed that air exposure and reimmersion had a significant impact on the oxygen consumption rate (OCR), ammonia excretion rate (AER), oxygen to nitrogen ratio (O:N), superoxide dismutase (SOD), catalase (CAT), succinate dehydrogenase (SDH), and lactate dehydrogenase (LDH). Significant interaction between air exposure and reimmersion was observed for OCR, AER, O:N, SOD, CAT, SDH, and LDH in Chinese mitten crab. During the air exposure stage, SOD, CAT, and LDH activities in the gills and hepatopancreas first increased and then decreased as air exposure time increased. All of these parameters were significantly higher in the 4-h air exposure group than those in the control group. All the parameters were significantly lower in the 16-h air exposure group than those in the control group, except LDH in the hepatopancreas. However, SDH activity gradually decreased with increased air exposure time, and all the air exposure groups were markedly lower than those in the control group in the gills. During the reimmersion stage, OCR, AER, and O:N restored to normal levels after 12-h reimmersion, except in the 16-h air exposure group, where OCR and O:N were significantly higher than those in the control group and AER was significantly lower than that in the control group. The LDH activity in all groups restored to normal levels after 12-h reimmersion. The SDH, SOD, and CAT activities of the 2- and 4-h air-exposed groups returned to normal levels after 12-h reimmersion; however, these three parameters were still significantly higher in the 16-h air-exposed group than in the control group in the gills and hepatopancreas. Overall, Chinese mitten crabs reduce aerobic respiration and increase anaerobic respiration capacity during desiccation. Under air exposure stress, Chinese mitten crabs change their energy utilization mode to meet their energy demands and adjust their respiratory metabolism and antioxidant enzymes activities to adapt to adverse environments.

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Antioxidative status, immunological responses, and heat shock protein expression in hepatopancreas of Chinese mitten crab, Eriocheir sinensis under the exposure of glyphosate

Cited by 63 publications

References 41 publications

Hepatopancreatic necrosis disease (HPND) in Chinese mitten crab Eriocheir sinensis tightly linked to low concentration of two insecticides

Hepatopancreatic necrosis disease (HPND) in Chinese mitten crab Eriocheir sinensis tightly linked to low concentration of two insecticides

Dual RNA-Seq Unveils Pseudomonas plecoglossicida htpG Gene Functions During Host-Pathogen Interactions With Epinephelus coioides

Respiratory Metabolism and Antioxidant Response in Chinese Mitten Crab Eriocheir sinensis During Air Exposure and Subsequent Reimmersion

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