Antiproliferation and cell apoptosis inducing bioactivities of constituents from Dysosma versipellis in PC3 and Bcap-37 cell lines

Abstract

BackgroundRecently, interest in phytochemicals from traditional Chinese medicinal herbs with the capability to inhibit cancer cells growth and proliferation has been growing rapidly due to their nontoxic nature. Dysosma versipellis as Bereridaceae plants is an endemic species in China, which has been proved to be an important Chinese herbal medicine because of its biological activity. However, systematic and comprehensive studies on the phytochemicals from Dysosma versipellis and their bioactivity are limited.ResultsFifteen compounds were isolated and characterized from the roots of Dysosma versipellis, among which six compounds were isolated from this plant for the first time. The inhibitory activities of these compounds were investigated on tumor cells PC3, Bcap-37 and BGC-823 in vitro by MTT method, and the results showed that podophyllotoxone (PTO) and 4'-demethyldeoxypodophyllotoxin (DDPT) had potent inhibitory activities against the growth of human carcinoma cell lines. Subsequent fluorescence staining and flow cytometry analysis indicated that these two compounds could induce apoptosis in PC3 and Bcap-37 cells, and the apoptosis ratios reached the peak (12.0% and 14.1%) after 72 h of treatment at 20 μM, respectively.ConclusionsThis study suggests that most of the compounds from the roots of D. versipellis could inhibit the growth of human carcinoma cells. In addition, PTO and DDPT could induce apoptosis of tumor cells.

Antiproliferative activity of yatein isolated fromAustrocedrus chilensisagainst murine myeloma cells: Cytological studies and chemical investigations

“…The most active compounds are phenolic diterpenes and lignans, and among them matairesinol, podophyllotoxin, yatein, ferruginol, and 6,7-dehydroferruginol (Banskota et al, 2003;Donoso et al, 2008, Donoso-Fierro et al, 2009. Lignans represent one of the most important and interesting classes of biologically active compounds, because lignan molecules can induce cancer cell apoptosis (Ayella et al, 2010;Chavez et al, 2011;Huong et al, 2011;Singh et al, 2007;Xu et al, 2006Xu et al, , 2011. Podophyllotoxin inhibits microtubule assembly of the mitotic apparatus, acting as a competitive inhibitor of the binding site for colchicine to tubulin (Loike et al, 1978); this process occurs during mitosis, where the microtubules are rearranged to form the mitotic spindle, which is essential for cell division (Ayres & Loik, 1990;Islam & Iskander, 2004;Schmidt & Bastians, 2007).…”

Antiproliferative and cell apoptosis-inducing activities of compounds from Buddleja davidii in Mgc-803 cells

“…Since AO is a crucial dye and can stain nuclear DNA across an unbroken cell membrane, whereas EB can only stain cells that had lost an intact cell membrane [43]. Thus, the following phenomena were observed after AO/EB staining: (1) viable cells have been uniformly stained green; (2) early apoptotic cells have been stained green 9 yellow or displayed green yellow fragments; (3) late apoptotic cells have been stained orange or displayed orange fragments; and (4) necrotic cells have been stained orange to red fluorescing nuclei with no indication of chromatin fragmentation.…”

“…Live cells with homogeneous light blue nuclei could be observed under fluorescence microscopy after staining with Hoechst 33258, and the apoptotic cells displayed bright blue due to karyopyknosis and chromatin condensation after treating with Hoechst 33258, and the nuclei of dead cells could not be stained [43]. As shown in Figure 5, all the mentioned changes could be observed.…”

“…TUNEL staining is a common method for detecting DNA fragmentation that results from apoptotic signaling cascades [44]. Apoptotic cells thus showing a brown color [43]. The experimental results are demonstrated in Figure 6.…”

Synthesis and biological evaluation of betulonic acid derivatives as antitumor agents

“…(4) The cells were washed three times with PBS, and were consequently incubated with diaminobenzidine solution (200 µL per specimen) for 10 min. At last, the cells were rewashed twice with PBS, and were consequentlyimaged under an XDS-1B inverted biological microscope[42].…”