Antisense Oligonucleotides as Therapeutic Agents

Alama, Angela; Barbieri, Federica; Cagnoli, Monica; Schettini, Gennaro

doi:10.1006/phrs.1997.0227

Cited by 52 publications

(35 citation statements)

References 33 publications

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“…These results are subject to the following criticisms: (i) they used a heterologous virus system, which may not faithfully represent events of HCV infection, and (ii) the actual mechanism(s) contributing to inhibition of HCV (luciferase) expression was not determined. However, considering the enormous number of applications of antisense strategies and the efficiency with which antisense transcripts disrupt gene expression (2,262), antisense targeting of HCV replication remains a viable means of developing anti-HCV therapies. Taken together, these results indicate that antisense oligonucleotides may provide a potent mechanism by which to target viral mRNA translation as an antiviral therapy.…”

Section: Mrna Translation As a Target For Antiviral Therapymentioning

confidence: 99%

Translational Control of Viral Gene Expression in Eukaryotes

Gale

Tan

Katze

2000

Microbiol Mol Biol Rev

299

255

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SUMMARY As obligate intracellular parasites, viruses rely exclusively on the translational machinery of the host cell for the synthesis of viral proteins. This relationship has imposed numerous challenges on both the infecting virus and the host cell. Importantly, viruses must compete with the endogenous transcripts of the host cell for the translation of viral mRNA. Eukaryotic viruses have thus evolved diverse mechanisms to ensure translational efficiency of viral mRNA above and beyond that of cellular mRNA. Mechanisms that facilitate the efficient and selective translation of viral mRNA may be inherent in the structure of the viral nucleic acid itself and can involve the recruitment and/or modification of specific host factors. These processes serve to redirect the translation apparatus to favor viral transcripts, and they often come at the expense of the host cell. Accordingly, eukaryotic cells have developed antiviral countermeasures to target the translational machinery and disrupt protein synthesis during the course of virus infection. Not to be outdone, many viruses have answered these countermeasures with their own mechanisms to disrupt cellular antiviral pathways, thereby ensuring the uncompromised translation of virion proteins. Here we review the varied and complex translational programs employed by eukaryotic viruses. We discuss how these translational strategies have been incorporated into the virus life cycle and examine how such programming contributes to the pathogenesis of the host cell.

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Section: Mrna Translation As a Target For Antiviral Therapymentioning

confidence: 99%

Translational Control of Viral Gene Expression in Eukaryotes

Gale

Tan

Katze

2000

Microbiol Mol Biol Rev

299

255

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“…If any or all of these genes are direct regulators of dierentiation, growth or the tumorigenic process then ectopically expressing these genes, using sense or inducible sense expression constructs (Jiang et al, 1995a(Jiang et al, ,c, 1996b(Jiang et al, , 1997, or blocking expression of these genes, using antisense or ribozyme based technologies (Alama et al, 1997;Su et al, 1998a), may directly modify these phenotypes in melanoma cells. Further studies designed to characterize the presently identi®ed DISH genes and to identify the remaining gene expression changes regulated as a function of induction of de®ned phenotypic changes in human melanoma cells are well warranted.…”

mentioning

confidence: 99%

Identification and temporal expression pattern of genes modulated during irreversible growth arrest and terminal differentiation in human melanoma cells

et al. 1999

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“…Antisense oligonucleotides have been investigated as therapeutics in various ocular diseases. 71,72 A sense oligonucleotide (ODN-1) targeted to a VEGF sequence was shown to reduce VEGF levels produced in hypoxic RPE cells, in vitro. In addition, this ODN-1 injected intravitreally in an animal model of CNV led to the attenuation of CNV, as assessed by fluorescein angiography.…”

Section: Dendrimers and Age-related Macular Degenerationmentioning

confidence: 99%

Dendrimers as tunable vectors of drug delivery systems and biomedical and ocular applications

Kalomiraki¹,

Thermos²,

Chaniotakis³

2015

IJN

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Abstract:Dendrimers are large polymeric structures with nanosize dimensions (1-10 nm) and unique physicochemical properties. The major advantage of dendrimers compared with linear polymers is their spherical-shaped structure. During synthesis, the size and shape of the dendrimer can be customized and controlled, so the finished macromolecule will have a specific "architecture" and terminal groups. These characteristics will determine its suitability for drug delivery, diagnostic imaging, and as a genetic material carrier. This review will focus initially on the unique properties of dendrimers and their use in biomedical applications, as antibacterial, antitumor, and diagnostic agents. Subsequently, emphasis will be given to their use in drug delivery for ocular diseases.

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Antisense Oligonucleotides as Therapeutic Agents

Cited by 52 publications

References 33 publications

Translational Control of Viral Gene Expression in Eukaryotes

Translational Control of Viral Gene Expression in Eukaryotes

Identification and temporal expression pattern of genes modulated during irreversible growth arrest and terminal differentiation in human melanoma cells

Dendrimers as tunable vectors of drug delivery systems and biomedical and ocular applications

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