Antisense telomerase RNA induced human gastric cancer cell apoptosis

Zhang, Fang Xin

doi:10.3748/wjg.v6.i3.430

Cited by 19 publications

(2 citation statements)

References 20 publications

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“…Many factors, such as high temperature, radiation, cytokine, anti-tumor chemotherapy agents, antibody, gene, etc. [12], [13], have a certain effect on modulating tumor cell cycle. Although verbascoside can arrest tumor cell growth, repair DNA oxidative damage and induce cell apoptosis and differentiation [6], [7], [14], [15], whether telomerase inhibition by verbascoside may lead to tumor cell apoptosis, and whether all the cell cycle changes exist as a common mechanism is not known.…”

Section: Resultsmentioning

confidence: 99%

In Vitro Modulation of Telomerase Activity, Telomere Length and Cell Cycle in MKN45 Cells by Verbascoside

Zhang

Jia²,

Deng³

et al. 2002

Planta med

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Screening of natural products with anti-tumor activity as telomerase inhibitor is a new subject in the field of tumor therapy. Using telomerase PCR ELISA, telomere DNA hybridization and flow cytometry analysis, the effects of verbascoside, a phenylpropanoid glucoside extracted from Pedicularis striata Pall, on telomerase activity, telomere length and cell cycle of human gastric carcinoma cells MKN45 was examined in vitro. After being treated with a 50 % inhibition concentration of verbascoside (17.8 microg/ml), telomerase activity in the cells was significantly inhibited but not in the cellular supernatant, the average telomere length became remarkably short, and the sub-G0 /G1 peak and G2/M arrest were also displayed when compared to the control cells. These results suggest that verbascoside mediated-cell differentiation and apoptosis may be affected by telomere-telomerase-cell cycle dependent modulation. Thus, the antitumor mechanism of verbascoside is demonstrated once more by its inhibiting effect on telomerase activity in tumor cells, and the telomerase assay may provide a valuable screening method for antitumor activity of natural products.

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Section: Resultsmentioning

confidence: 99%

In Vitro Modulation of Telomerase Activity, Telomere Length and Cell Cycle in MKN45 Cells by Verbascoside

Zhang

Jia²,

Deng³

et al. 2002

Planta med

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“…The results suggested that the formation of MDR in SGC7901/VCR cells was associated with over expression of ZNRD1. Because gastric cancer is common in China and some areas in the world [14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33] , these results may be important for further study.…”

Section: Discussionmentioning

confidence: 99%

Effect of ZNRD1 gene antisense RNA on drug resistant gastric cancer cells

Zhang

Zhao²,

Pan³

et al. 2003

WJG

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AIM:To investigate the expression level of ZNRD1 gene in gastric cancer cells SGC7901 and gastric cancer MDR (multidrug resistant) cells SGC7901/VCR, and to observe the drug sensitizing and proliferation effect of ZNRD1 antisense nucleic acid transduction on SGC7901/VCR cells. METHODS:Amplification of sequences encoding ZNRD1 from SGC7901/VCR cDNA by PCR. The levels of ZNRD1 mRNA expression were demonstrated using semiquantitative reverse transcription polymerase chain reaction (RT-PCR). Eukaryotic expression vector pcDNA3.1-anti ZNRD1 was constructed and transfected into SGC7901/VCR cells by lipofectamine. Immunochemical method was used to detect the expression of protein in SGC7901/VCR cells and transfectants. The cell cycle alteration and the intracellular adriamycin (ADM) accumulation were observed by FACS. Growth curve and drug sensitization of cells for vincristine (VCR) were analyzed with MTT assay. RESULTS:We cloned the open reading frame of full-length ZNRD1. The expression of ZNRD1 showed higher in SGC7901/VCR than in SGC7901 cells. The antisense ZNRD1 drug-resistant clones were selected after gene transfection. Immunochemical results showed that the expression level of ZNRD1 protein was lower in anti ZNRD1-SGC7901/VCR cells than that in non-transfectants. Comparing to SGC7901/ VCR and pcDNA3

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Targeting telomerase for its advent in cancer therapeutics

Bajaj

Kumar

Peters

et al. 2020

Medicinal Research Reviews

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Telomerase has emerged as an important primary target in anticancer therapy. It is a distinctive reverse transcriptase enzyme, which extends the length of telomere at the 3′ chromosomal end, and uses telomerase reverse transcriptase (TERT) and telomerase RNA template‐containing domains. Telomerase has a vital role and is a contributing factor in human health, mainly affecting cell aging and cell proliferation. Due to its unique feature, it ensures unrestricted cell proliferation in malignancy and plays a major role in cancer disease. The development of telomerase inhibitors with increased specificity and better pharmacokinetics is being considered to design and develop newer potent anticancer agents. Use of natural and synthetic compounds for the inhibition of telomerase activity can lead to an opening of new vistas in cancer treatment. This review details about the telomerase biochemistry, use of natural and synthetic compounds; vaccines and oncolytic virus in therapy that suppress the telomerase activity. We have discussed structure‐activity relationships of various natural and synthetic telomerase inhibitors to help medicinal chemists and chemical biology researchers with a ready reference and updated status of their clinical trials. Suppression of human TERT (hTERT) activity through inhibition of hTERT promoter is an important approach for telomerase inhibition.

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Antisense telomerase RNA induced human gastric cancer cell apoptosis

Cited by 19 publications

References 20 publications

In Vitro Modulation of Telomerase Activity, Telomere Length and Cell Cycle in MKN45 Cells by Verbascoside

In Vitro Modulation of Telomerase Activity, Telomere Length and Cell Cycle in MKN45 Cells by Verbascoside

Effect of ZNRD1 gene antisense RNA on drug resistant gastric cancer cells

Targeting telomerase for its advent in cancer therapeutics

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