Antitumor effect and underlying mechanism of RGD-modified adenovirus mediated IL-24 expression on myeloid leukemia cells

Yu, Xin; Xia, Wei; Li, Shuai; Blumenfeld, Julie; Zhang, Biao; Yang, Jicheng; Jiang, Miao; Gu, Zhifeng

doi:10.1016/j.intimp.2015.07.014

Cited by 5 publications

(4 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The recombinant adenovirus vector was constructed on the basis of a previously reported method (Yu et al, ). The VEGF165 and Ang‐1 genes were ligated into the pAdTrack‐CMV‐PolyA‐promoter shuttle vector expressing GFP to form pAdTrack‐CMV‐VEGF165‐PolyA‐promoter‐Ang‐1 (Ma et al, ).…”

Section: Methodsmentioning

confidence: 99%

“…The recombinant adenovirus vector was constructed on the basis of a previously reported method (Yu et al, 2015). The VEGF165 and Ang-1 genes were ligated into the pAdTrack-CMV-PolyA-promoter shuttle vector expressing GFP to form pAdTrack-CMV-VEGF165-PolyA-promoter-Ang-1 (Ma et al, 2014) The titres of the adenovirus vectors were determined following previously reported methods (He et al, 1998;Hitt et al, 2000;Nyberg-Hoffman, Shabram, Li, Giroux, & Aguilar-Cordova, 1997;Philipson, 1961).…”

Section: Construction Of Recombinant Adenovirus Vectorsmentioning

confidence: 99%

See 1 more Smart Citation

Silk fibroin scaffolds loaded with angiogenic genes in adenovirus vectors for tissue regeneration

Wang

Jiang

et al. 2019

J Tissue Eng Regen Med

Self Cite

View full text Add to dashboard Cite

Vascularization remains a critical challenge in dermal tissue regeneration. In this study, a vascular endothelial growth factor (VEGF165) and angiopoietin‐1 (Ang‐1) dual gene coexpression vector that encoded green fluorescent protein (GFP) was constructed from an arginine–glycine–aspartic acid‐modified adenovirus. Silk fibroin (SF) scaffolds loaded with adenovirus vectors were fabricated by freeze‐drying method. In vitro, the human endothelial‐derived cell line EA.hy926 was infected with adenovirus vectors and then expressed GFP, secreted VEGF165 and Ang‐1, and promoted cell proliferation effectively. The VEGF165 and Ang‐1 genes loaded in the SF scaffolds significantly promoted the formation of abundant microvascular networks in the chick embryo chorioallantoic membrane. In vivo, angiogenic genes loaded in the scaffolds promoted vascularization and collagen deposition in scaffolds, thus effectively accelerating dermal tissue regeneration in a dorsal full‐thickness skin defect wound model in Sprague–Dawley rats. In conclusion, SF scaffolds loaded with arginine–glycine–aspartic acid‐modified adenovirus vectors encoding VEGF165 and Ang‐1 could stimulate the formation of vascular networks through the effective expression of target genes in vascular endothelial cells, thereby accelerating the regeneration of dermal tissue.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Construction Of Recombinant Adenovirus Vectorsmentioning

confidence: 99%

Silk fibroin scaffolds loaded with angiogenic genes in adenovirus vectors for tissue regeneration

Wang

Jiang

et al. 2019

J Tissue Eng Regen Med

Self Cite

View full text Add to dashboard Cite

show abstract

“…Several studies have reported the potential of MDA7/IL-24 in inducing CTL proliferation [ 13 ], and apoptosis through the induction of mitochondrial apoptosis pathways and/or endoplasmic reticulum (ER) stress [ 39 – 41 ]. In one study, the antitumor effects of this cytokine against breast cancer were demonstrated to be applied through apoptosis and stress induction in ER, as well as the inhibition of cell growth and suppression of the self-renewal ability of cancer stem cells [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

Enhanced synergistic antitumor effect of a DNA vaccine with anticancer cytokine, MDA-7/IL-24, and immune checkpoint blockade

Miri

Pourhossein

Hosseini

et al. 2022

Virol J

View full text Add to dashboard Cite

Background MDA-7/IL-24 cytokine has shown potent antitumor properties in various types of cancer without exerting any significant toxicity on healthy cells. It has also been proved to encompass pro-immune Th1 cytokine-like behavior. Several E7 DNA vaccines have developed against human papillomavirus (HPV)-related cervical cancer. However, the restricted immunogenicity has limited their clinical applications individually. To address this deficiency, we investigated whether combining the E7 DNA vaccine with MDA-7/IL-24 as an adjuvant would elicit efficient antitumor responses in tumor-bearing mouse models. Next, we evaluated how suppression of immunosuppressive IL-10 cytokine would enhance the outcome of our candidate adjuvant vaccine. Methods For this purpose, tumor-bearing mice received either E7 DNA vaccine, MDA-7/IL-24 cytokine or combination of E7 vaccine with MDA-7/IL-24 adjuvant one week after tumor challenge and boosted two times with one-week interval. IL-10 blockade was performed by injection of anti-IL-10 mAb before each immunization. One week after the last immunization, mice were sacrificed and the treatment efficacy was evaluated through immunological and immunohistochemical analysis. Moreover, the condition of tumors was monitored every two days for six weeks intervals from week 2 on, and the tumor volume was measured and compared within different groups. Results A highly significant synergistic relationship was observed between the E7 DNA vaccine and the MDA-7/IL-24 cytokine against HPV-16+ cervical cancer models. An increase in proliferation of lymphocytes, cytotoxicity of CD8+ T cells, the level of Th1 cytokines (IFN-γ, TNF-α) and IL-4, the level of apoptotic markers (TRAIL and caspase-9), and a decrease in the level of immunosuppressive IL-10 cytokine, together with the control of tumor growth and the induction of tumor regression, all prove the efficacy of adjuvant E7&IL-24 vaccine when compared to their individual administration. Surprisingly, vaccination with the DNA E7&IL-24 significantly reduced the population of Regulatory T cells (Treg) in the spleen of immunized mice compared to sole administration and control groups. Moreover, IL-10 blockade enhanced the effect of the co-administration by eliciting higher levels of IFN-γ and caspase-9, reducing Il-10 secretion and provoking the regression of tumor size. Conclusion The synergy between the E7 DNA vaccine and MDA-7/IL-24 suggests that DNA vaccines’ low immunogenicity can be effectively addressed by coupling them with an immunoregulatory agent. Moreover, IL-10 blockade can be considered a complementary treatment to improve the outcome of conventional or novel cancer therapies.

show abstract

“…Interleukin 24 (IL-24), a newly identified antitumor gene, can inhibit the growth of tumor cells, including lung, breast and ovarian cancer cells, and is considered to be combinable with radiotherapy or chemotherapy, which could improve the effects of radiotherapy and chemotherapy on tumor cells ( 15 – 17 ). The IL-24 gene has been widely investigated in various cancers for its role as a tumor-suppressor gene, particularly with regard to tumor gene therapy ( 18 ); however, its role in reversing MDR has not been investigated in detail. In the present study, we used adenovirus-mediated human IL-24 gene (Ad-hIL-24) transfection and the cisplatin (DDP)-resistant human lung adenocarcinoma cell line A549/DDP to study whether IL-24 can reverse the MDR of lung cancer as well as investigate its mechanism.…”

Section: Introductionmentioning

confidence: 99%

Reversal effect of adenovirus-mediated human interleukin 24 transfection on the cisplatin resistance of A549/DDP lung cancer cells

Tang

Sun

et al. 2017

Oncology Reports

View full text Add to dashboard Cite

Interleukin-24 (IL-24) is a tumor-suppressor gene that has been documented in human melanoma cells. IL-24 has marked antitumor activities on various types of human cancer, but its underlying mechanism remains unclear. In the present, we investigated the effects of human IL-24 (hIL-24) on the chemotherapy resistance of lung cancer cells. The cisplatin (DDP)-resistant lung carcinoma cell line A549/DDP was subjected to adenovirus-mediated transfection with the human IL-24 gene (Ad-hIL-24). The growth-inhibitory and apoptotic effects of Ad-hIL-24 on A549/DDP cells were observed, and the expression levels of AKT, phosphorylated-AKT (p-AKT) and P-glycoprotein (P-gp) were detected. Ad-hIL-24 significantly decreased the levels of p-AKT and P-gp, and effectively inhibited A549/DDP cell growth. Furthermore, A549/DDP cells exhibited a significantly increased rate of apoptosis, as well as G2/M-phase arrest, following transfection with Ad-hIL-24, and these effects were increased in cells treated with Ad-IL-24 combined with DDP when compared with those treated with Ad-hIL-24 or DDP alone. These results suggest that hIL-24 can reverse the DDP resistance of lung cancer cells, and that the associated mechanism involves the induction of apoptosis and G2/M-phase arrest through the phosphoinositide3-kinase (PI3K)/AKT signaling pathway, as well as a decrease in drug resistance through P-gp expression.

show abstract

Antitumor effect and underlying mechanism of RGD-modified adenovirus mediated IL-24 expression on myeloid leukemia cells

Cited by 5 publications

References 31 publications

Silk fibroin scaffolds loaded with angiogenic genes in adenovirus vectors for tissue regeneration

Silk fibroin scaffolds loaded with angiogenic genes in adenovirus vectors for tissue regeneration

Enhanced synergistic antitumor effect of a DNA vaccine with anticancer cytokine, MDA-7/IL-24, and immune checkpoint blockade

Reversal effect of adenovirus-mediated human interleukin 24 transfection on the cisplatin resistance of A549/DDP lung cancer cells

Contact Info

Product

Resources

About