Antitussive, antispasmodic, bronchodilating and cardiac inotropic effects of the essential oil from Blepharocalyx salicifolius leaves

Hernández, Jehison Jiménez; Ragone, María Inés; Bonazzola, Patricia; Bandoni, Arnaldo L.; Consolini, Alicia E.

doi:10.1016/j.jep.2017.08.013

Cited by 19 publications

(10 citation statements)

References 38 publications

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“…These results for major compounds are similar to those reported in the literature regarding globulol [ 17 , 18 ], γ-eudesmol and α-eudesmol [ 22 ], ( E )-caryophyllene [ 21 , 23 ] and α-pinene [ 17 , 18 , 20 , 21 , 27 ]. Other terpenes, such as bicyclogermacrene, viridiflorol, rosifoliol, cubeban-11-ol and palustrol, are being described here for the first time as major components of the essential oil in leaves of B. salicifolius .…”

Section: Resultssupporting

confidence: 90%

“…The chemical composition of the essential oil in leaves extracted from this species has been reported and mainly includes 1,8-cineol, α-pinene, limonene, β-pinene, α-terpineol, ( E )-caryophyllene and linalool [ 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 ]. However, few studies relating to the biological activities of the essential oil from this plant are available.…”

Section: Introductionmentioning

confidence: 99%

“…However, few studies relating to the biological activities of the essential oil from this plant are available. Until now, only fungistatic activity against Phyllosticta citricarpa and antitussive, antispasmodic, bronchodilating and cardiac inotropic effects have been described for the essential oil from leaves of this plant [ 27 , 28 ].…”

Section: Introductionmentioning

confidence: 99%

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Chemical Composition and Bioactivity of Essential Oil from Blepharocalyx salicifolius

Furtado

Borges

Teixeira

et al. 2018

IJMS

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Natural products represent a source of biologically active molecules that have an important role in drug discovery. The aromatic plant Blepharocalyx salicifolius has a diverse chemical constitution but the biological activities of its essential oils have not been thoroughly investigated. The aims of this paper were to evaluate in vitro cytotoxic, antifungal and antibacterial activities of an essential oil from leaves of B. salicifolius and to identify its main chemical constituents. The essential oil was extracted by steam distillation, chemical composition was determined by gas chromatography/mass spectrometry, and biological activities were performed by a microdilution broth method. The yield of essential oil was 0.86% (w/w), and the main constituents identified were bicyclogermacrene (17.50%), globulol (14.13%), viridiflorol (8.83%), γ-eudesmol (7.89%) and α-eudesmol (6.88%). The essential oil was cytotoxic against the MDA-MB-231 (46.60 μg·mL−1) breast cancer cell line, being more selective for this cell type compared to the normal breast cell line MCF-10A (314.44 μg·mL−1). Flow cytometry and cytotoxicity results showed that this oil does not act by inducing cell death, but rather by impairment of cellular metabolism specifically of the cancer cells. Furthermore, it presented antifungal activity against Paracoccidioides brasiliensis (156.25 μg·mL−1) but was inactive against other fungi and bacteria. Essential oil from B. salicifolius showed promising biological activities and is therefore a source of molecules to be exploited in medicine or by the pharmaceutical industry.

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Section: Resultssupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Chemical Composition and Bioactivity of Essential Oil from Blepharocalyx salicifolius

Furtado

Borges

Teixeira

et al. 2018

IJMS

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“…( Table 1). The sample CH1 sold as C. sinensis did not correspond to this species but to Blepharocalyx tweediei [30] Berg. Blepharocalyx tweediei is a tree native to Argentina and Uruguay that is traditionally used as infusion for cough, bronchospasm, diarrhea, and other intestinal disorders.…”

Section: Dna Barcoding Analysismentioning

confidence: 89%

DNA-Based Authentication and Metabolomics Analysis of Medicinal Plants Samples by DNA Barcoding and Ultra-High-Performance Liquid Chromatography/Triple Quadrupole Mass Spectrometry (UHPLC-MS)

Sánchez

González-Burgos

Divakar

et al. 2020

Plants

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There is growing interest for medicinal plants in the world drug market. Particularly, Matricaria recutita L., Valeriana officinalis L., Tilia spp., and Camellia sinensis (L.) Kuntze are some of the most consumed medicinal plants for treatment of minor health problems. Medicinal plants are seen as natural and safe; however, they can cause interactions and produce adverse reactions. Moreover, there is lack of consensus in medicinal plants regulation worldwide. DNA barcoding and UHPLC-MS technique are increasingly used to correctly identify medicinal plants and guarantee their quality and therapeutic safety. We analyzed 33 samples of valerian, linden, tea, and chamomile acquired in pharmacies, supermarkets, and herbal shops by DNA barcoding and UHPLC-MS. DNA barcoding, using matk as a barcode marker, revealed that CH1 sold as Camellia sinensis was Blepharocalyx tweediei, and sample TS2 sold as linden belong to Malvales. On the other hand, UHPLC-MS analysis revealed the presence of bioactive compounds (apigenin-7-glucoside, acetoxy valerenic acid, valerenic acid, epigallocatechin, and tiliroside). However, none of samples met minimum content of these active principles (except for valerenic acid in VF3) according to the European Medicines Agency (EMA) and Real Spanish Pharmacopeia. In conclusion, this study revealed the need to incorporate DNA barcoding and HPLC-MS techniques in quality controls of medicinal plants.

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“…Then, rats were quickly sacrificed by opening the thorax and abdomen. The duodenum and ileum (about 2 cm long) were excised and mounted in 20 ml chambers for isolated organs containing Tyrode's solution at 37 ºC and constantly oxygenated with air bubbling, as in previous works (Ragone et al, 2007;Matera et al, 2012;Blanco et , 2013;Jiménez Hernández et al, 2018). The excised bladder was divided in 3 or 4 longitudinal slices respectively mounted in chambers with Tyrode´s solution similarly to the intestinal portions.…”

Section: Pharmacological Studies Biological Preparations and Contractmentioning

confidence: 99%

Intestinal, urinary and uterine antispasmodic effects of isoespintanol, metabolite from Oxandra xylopioides leaves

Buñay¹,

Colareda²,

Ragone³

et al. 2018

Phytomedicine

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Consolini , Intestinal, urinary and uterine antispasmodic effects of isoespintanol, metabolite from Oxandra xylopioides leaves, Phytomedicine (2018), ABSTRACTBackground: Isoespintanol is a monoterpene isolated from the leaves of Oxandra xylopioides Diels. (Annonaceae) with antioxidant and antiinflammatory effects. It was of interest to know whether it has antispasmodic effects such as other known drugs, phloroglucinol and trimethoxybenzene, used in therapeutics for treating biliary, urinary and uterine spasms.Purpose: to assess whether isoespintanol possesses antispasmodic effects on intestine, uterus and bladder.Study design: A preclinical study was performed in which isoespintanol, phloroglucinol and trimethoxybenzene were evaluated with concentration-contractile response curves (CRC) of carbachol in isolated rat intestine and bladder, and with CRC of serotonin (5-HT) in rat uterus.Moreover, it was assessed whether isoespintanol interferes with Ca 2+ influx by making CRC of Ca 2+ in high-K + medium in intestine and bladder.Results: isoespintanol non-competitively inhibited the CRC of carbachol with affinity constant (pK) of 4.78 ± 0.09 in intestine and 4.60 ± 0.09 in bladder. Phloroglucinol and trimethoxybenzene were also non-competitive antagonists, but isoespintanol was 8 times more potent than trimethoxybenzene and similarly potent than phloroglucinol in intestine. In bladder, isoespintanol resulted 8 times more potent than trimethoxybenzene. The maximal inhibition of contraction followed the order of isoespintanol > trimethoxybenzene > phloroglucinol in intestine, and isoespintanol > trimethoxybenzene in bladder. Moreover, isoespintanol also completely and non-competitively inhibited the CRC of Ca 2+ , with a pK of 5.1 ± 0.1 in intestine, and 4.32 ± 0.07 in bladder. In uterus isoespintanol reduced, completely and non-competitively, the contraction produced by 5-HT with pK of 5.05 ± 0.07. Conclusions:Results demonstrate that isoespintanol is a very good intestinal, urinary and uterine antispasmodic, with higher potency than the other drugs used in therapeutics. The mechanism of action of isoespintanol is the interference with Ca 2+ influx, at a difference of ACCEPTED MANUSCRIPT A C C E P T E D M A N U S C R I P T 3 trimethoxybenzene and phloroglucinol.

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Antitussive, antispasmodic, bronchodilating and cardiac inotropic effects of the essential oil from Blepharocalyx salicifolius leaves

Cited by 19 publications

References 38 publications

Chemical Composition and Bioactivity of Essential Oil from Blepharocalyx salicifolius

Chemical Composition and Bioactivity of Essential Oil from Blepharocalyx salicifolius

DNA-Based Authentication and Metabolomics Analysis of Medicinal Plants Samples by DNA Barcoding and Ultra-High-Performance Liquid Chromatography/Triple Quadrupole Mass Spectrometry (UHPLC-MS)

Intestinal, urinary and uterine antispasmodic effects of isoespintanol, metabolite from Oxandra xylopioides leaves

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