Antivenom action on renal effects induced by Thalassophryne nattereri venom

Martins, Alice Maria Costa; Barbosa, Paula; Sousa, Daniel Freire de; Alves, Claudênio Diógenes; Menezes, Dalgimar Beserra de; Lima, Carla; Lopes-Ferreira, Mônica; Fonteles, M. C.; Monteiro, Helena Serra Azul

doi:10.1590/s1678-91992009000100011

Cited by 3 publications

(4 citation statements)

References 16 publications

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“…Changes in kidney function include increase in renal parameters such as perfusion pressure, vascular resistance, glomerular filtration rate and urinary flow. Renal changes observed in this study corroborate earlier reports with other animal venoms which registered similar changes in renal function (24,25).…”

Section: Discussionsupporting

confidence: 93%

Renal- and calcium-dependent vascular effects of Polybia paulista wasp venom

Vinhote¹,

Torres²,

Dantas³

et al. 2011

J. Venom. Anim. Toxins incl. Trop. Dis

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Abstract:In the present study, the effects of Polybia paulista venom (PPV) on renal and vascular tissues were investigated. Isolated kidneys perfused with PPV (1 and 3 µg/mL) had increased perfusion pressure, renal vascular resistance , urinary flow, and glomerular filtration rate; and reduced sodium tubular transport. Histological evaluation demonstrated deposits of proteins in Bowman's space and tubular lumen, and focal areas of necrosis. The venom promoted a cytotoxic effect on Madin-Darby canine kidney (MDCK) cells. A significant increase in lactic dehydrogenase levels was observed in response to venom exposure. In isolated mesenteric vascular beds, pressure and vascular resistance augmented in a dose-dependent manner. PPV increased the contractility of aortic rings maintained under basal tension. This contractile response was inhibited when preparations were maintained in Ca 2+ -free medium. Likewise, verapamil, a voltage-gated calcium channel blocker, also inhibited the contractile response. In this study, phentolamine, a blocker of α-adrenergic receptor blocker, significantly reduced the contractile effect of PPV in the aortic ring. In conclusion, PPV produced nephrotoxicity, which suggests a direct effect on necrotic cellular death in renal tubule cells. The vascular contractile effect of PPV appears to involve calcium influx through voltage-gated calcium channels via adrenergic regulation.

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Section: Discussionsupporting

confidence: 93%

Renal- and calcium-dependent vascular effects of Polybia paulista wasp venom

Vinhote¹,

Torres²,

Dantas³

et al. 2011

J. Venom. Anim. Toxins incl. Trop. Dis

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“…We may suggest that the venom affects directly kidney cells by causing the release of vasoactive factors and the decrease of electrolyte transport [10,11]. …”

Section: Reviewmentioning

confidence: 99%

“…Additionally we demonstrated that T. nattereri venom (0.3, 1.0, and 3.0 μg/mL) promoted increase in perfusion pressure and renal vascular resistance in mice with hyalin casts inside all tubules and proteinaceous material in the urinary space. We may suggest that the venom affects directly kidney cells by causing the release of vasoactive factors and the decrease of electrolyte transport [ 10 , 11 ].…”

Section: Reviewmentioning

confidence: 99%

Thalassophryne nattereri fish venom: from the envenoming to the understanding of the immune system

Lopes-Ferreira

Grund

Lima

2014

J Venom Anim Toxins incl Trop Dis

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Thalassophryne nattereri (niquim) is a venomous fish found off North and Northeast coast of Brazil, where it is known by the severity of the accidents involving humans. This review article is divided into four topics. The first one provides a brief description of the animal biology and its distribution off Brazilian coastal waters, the venom apparatus, signs and symptoms observed in envenomated humans and also describes envenomation in mice. The second topic describes the use of modern genetic approach and mass spectrometry for identification of highly expressed genes in its venom glands and the sequence of major toxins. The third chapter offers a detailed study of tissue injury induced by the venom and reveals the role of toxins that impair inflammation reduction. Finally, the fourth section expands the understanding of many extrinsic and intrinsic essential factors in maintaining survival of memory B cell compartment. Our results demonstrate the wide possibilities for research in the area of toxinology, also the necessity of interconnection among biochemistry, pharmacology and immunology areas for the expansion of knowledge and for generation of innovation.

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“…Although fish envenomation is frequent in Brazil, only pre-clinical studies of neutralization with anti-venom antibodies in experimental models were performed [9,[19][20][21][22], and until now there are no commercially available antisera for human victims. The objective of this work was to produce an antiserum in mice composed of polyclonal IgG antibodies against multiple toxins from the venoms of the most medically important fish species in Brazil such as Thalassophryne nattereri, Scorpaena plumieri, Potamotrygon gr.…”

Section: Introductionmentioning

confidence: 99%

Effective Pre-Clinical Treatment of Fish Envenoming with Polyclonal Antiserum

Ferreira

Falcão

Bruni

et al. 2023

IJMS

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Envenomation by venomous fish, although not always fatal, is capable of causing damage to homeostasis by activating the inflammatory process, with the formation of edema, excruciating pain, necrosis that is difficult to heal, as well as hemodynamic and cardiorespiratory changes. Despite the wide variety of pharmacological treatments used to manage acute symptoms, none are effective in controlling envenomation. Knowing the essential role of neutralizing polyclonal antibodies in the treatment of envenoming for other species, such as snakes, this work aimed to produce a polyclonal antiserum in mice and test its ability to neutralize the main toxic effects induced by the venoms of the main venomous Brazilian fish. We found that the antiserum recognizes the main toxins present in the different venoms of Thalassophryne nattereri, Scorpaena plumieri, Potamotrygon gr. Orbignyi, and Cathorops spixii and was effective in pre-incubation trials. In an independent test, the antiserum applied immediately to the topical application of T. nattereri, P. gr orbygnyi, and C. spixii venoms completely abolished the toxic effects on the microcirculation, preventing alterations such as arteriolar contraction, slowing of blood flow in postcapillary venules, venular stasis, myofibrillar hypercontraction, and increased leukocyte rolling and adherence. The edematogenic and nociceptive activities induced by these venoms were also neutralized by the immediate application of the antiserum. Importantly, the antiserum prevented the acute inflammatory response in the lungs induced by the S. plumieri venom. The success of antiserum containing neutralizing polyclonal antibodies in controlling the toxic effects induced by different venoms offers a new strategy for the treatment of fish envenomation in Brazil.

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Antivenom action on renal effects induced by Thalassophryne nattereri venom

Cited by 3 publications

References 16 publications

Renal- and calcium-dependent vascular effects of Polybia paulista wasp venom

Renal- and calcium-dependent vascular effects of Polybia paulista wasp venom

Thalassophryne nattereri fish venom: from the envenoming to the understanding of the immune system

Effective Pre-Clinical Treatment of Fish Envenoming with Polyclonal Antiserum

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