AOAC SMPR 2016.010:Standard Method Performance Requirements (SMPRs) for DNA-Based Methods of Detecting Burkholderia pseudomallei in Field-Deployable, Department of Defense Aerosol
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Gee, Jay E.; Arce, Jennifer; Beck, Linda; Blank, Thomas R.; Blyn, Larry B.; Cahall, Ryan; Clark, Amanda J.; Currie, Bart J.; Damer, Kenneth; Davenport, Matthew; DeShazer, David; Johns, Malcolm; Keim, Paul; Kiss, Katalin; Lesho, Matthew; Lin, Nancy J.; Morse, Stephen; Naraghi-Arani, Pejman; Ozanich, Richard M.; Roberto, Francisco; Rozak, David A.; Sahl, Jason W.; Schaefer, Frank W.; Schutzer, Steven E.; Schweizer, Herbert P.; Sozhamannan, Shanmuga; Tuanyok, Apichai; Coates, Scott G

doi:10.5740/jaoacint.smpr2016.010

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“…This strain was known to be negative to a species-specific TTS1 PCR assay for B. pseudomallei [58]. The strain was selected for inclusion on the inclusivity panel of B. pseudomallei strains tested for DNA-based assay development [59]. A further analysis by us using its genome to compare with the genome of K96243 has shown that it had lost approximately 273 genes starting from bpss1331 to bpss1603 in chromosome 2.…”

Section: Plos Neglected Tropical Diseasesmentioning

confidence: 99%

Genomic loss in environmental and isogenic morphotype isolates of Burkholderia pseudomallei is associated with intracellular survival and plaque-forming efficiency

et al. 2020

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Background Burkholderia pseudomallei is an environmental bacterium that causes melioidosis. A facultative intracellular pathogen, B. pseudomallei can induce multinucleated giant cells (MNGCs) leading to plaque formation in vitro. B. pseudomallei can switch colony morphotypes under stress conditions. In addition, different isolates have been reported to have varying virulence in vivo, but genomic evolution and the relationship with plaque formation is poorly understood. Methodology/Principle findings To gain insights into genetic underpinnings of virulence of B. pseudomallei, we screened plaque formation of 52 clinical isolates and 11 environmental isolates as well as 4 isogenic morphotype isolates of B. pseudomallei strains K96243 (types II and III) and 153 (types II and III) from Thailand in A549 and HeLa cells. All isolates except one environmental strain (A4) and K96243 morphotype II were able to induce plaque formation in both cell lines. Intracellular growth assay and confocal microscopy analyses demonstrated that the two plaqueforming-defective isolates were also impaired in intracellular replication, actin polymerization and MNGC formation in infected cells. Whole genome sequencing analysis and PCR revealed that both isolates had a large genomic loss on the same region in chromosome 2, which included Bim cluster, T3SS-3 and T6SS-5 genes.

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Section: Plos Neglected Tropical Diseasesmentioning

confidence: 99%