API System: a Multitube Micromethod for Identification of
            <i>Enterobacteriaceae</i>

Smith, P. B.; Tomfohrde, K. M.; Rhoden, D. L.; Balows, Albert

doi:10.1128/am.24.3.449-452.1972

Cited by 155 publications

(26 citation statements)

References 3 publications

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“…Enzymatic activities such as catalase and oxydase activities were analysed with a BBL DrySlide following manufacturer's instructions (Becton Dickinson, Le Pont de Claix, France). API strips (API ZYM 40 – 42 , API 20NE 43 , 44 , API 20E 45 , 46 and API 50CH 47 – 50 , bioMérieux) were used to study strains biochemical characteristics.…”

Section: Methodsmentioning

confidence: 99%

Chryseobacterium schmidteae sp. nov. a novel bacterial species isolated from planarian Schmidtea mediterranea

Kangale

Raoult

Ghigo

et al. 2021

Sci Rep

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Marseille-P9602T is a Chryseobacterium-like strain that we isolated from planarian Schmidtea mediterranea and characterized by taxono-genomic approach. We found that Marseille-P9602T strain exhibits a 16S rRNA gene sequence similarity of 98.76% with Chryseobacterium scophthalmum LMG 13028T strain, the closest phylogenetic neighbor. Marseille-P9602T strain was observed to be a yellowish-pigmented, Gram-negative, rod-shaped bacterium, growing in aerobic conditions and belonging to the Flavobacteriaceae family. The major fatty acids detected are 13-methyl-tetradecanoic acid (57%), 15-methylhexadecenoic acid (18%) and 12-methyl-tetradecanoic acid (8%). Marseille-P9602 strain size was found from genome assembly to be of 4,271,905 bp, with a 35.5% G + C content. The highest values obtained for Ortho-ANI and dDDH were 91.67% and 44.60%, respectively. Thus, hereby we unravel that Marseille-P9602 strain is sufficiently different from other closed related species and can be classified as a novel bacterial species, for which we propose the name of Chryseobacterium schmidteae sp. nov. Type strain is Marseille-P9602T (= CSUR P9602T = CECT 30295T).

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Section: Methodsmentioning

confidence: 99%

Chryseobacterium schmidteae sp. nov. a novel bacterial species isolated from planarian Schmidtea mediterranea

Kangale

Raoult

Ghigo

et al. 2021

Sci Rep

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“…The catalase test was not used in this study. A complete description of the strip is given in other reports (1,5,10,12).…”

Section: Methodsmentioning

confidence: 99%

Evaluation of the API 20E system for identification of nonfermentative Gram-negative bacteria

Shayegani¹,

Maupin²,

McGlynn³

1978

J Clin Microbiol

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The API 20E system for Enterobacteriaceae, recently broadened to include identification of nonfermentative gram-negative bacteria, was evaluated and compared with the conventional method for complete identification of 221 nonfermenters, which were well distributed into 48 species or biotypes and included organisms not listed in the API 20E data base. The results of 16 tests common to both systems were in close agreement. The API 20E system correctly identified 71 (43%) of the 165 organisms included in the API 20E data base. However, almost 90% of Acinetobacter calcoaceticus, three species of Pseudomonas, and Bordetella bronchiseptica were correctly identified to species.

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“…Catalase and oxidase activities were tested using a BBL DrySlide according to the manufacturer's instructions (Becton Dickinson, Le Pont de Claix, France). API strips (API ZYM [34][35][36], API 20NE [37,38], API 20E [39,40] and API 50CH [41][42][43][44], bioMérieux) were used to study the biochemical characteristics for Marseille-P9898 strain and Metabacillus niabensis strain 4T19 T [45].…”

Section: Phenotypic and Biochemical Featuresmentioning

confidence: 99%

Metabacillus schmidteae sp. nov., Cultivated from Planarian Schmidtea mediterranea Microbiota

Kangale

Raoult

Ghigo

et al. 2021

Microbiology Research

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Taxonogenomics combines phenotypic assays and genomic analysis as a means of characterizing novel strains. We used this strategy to study Marseille-P9898T strain, an aerobic, motile, Gram-negative, spore-forming, and rod-shaped bacterium isolated from planarian Schmidtea mediterranea. Marseille-P9898T is catalase-positive and oxidase-negative. The major fatty acids detected are 12-methyl-tetradecanoic acid, 13-methyl-tetradecanoic acid, and hexadecanoic acid. Marseille-P9898T strain shared more than 98% sequence similarity with the Metabacillus niabensis strain 4T19T (98.99%), Metabacillus halosaccharovorans strain E33T (98.75%), Metabacillus malikii strain NCCP-662T (98.19%), and Metabacillus litoralis strain SW-211T (97.15%). Marseille-P9898 strain belongs to Metabacillus genus. Genomic analysis revealed the highest similarities with Ortho-ANI and dDDH, 85.76% with Metabacillus halosaccharovorans, and 34.20% with Bacillus acidicola, respectively. These results show that the Marseille-P9898T strain is a novel bacterial species from Metabacillus genus, for which we propose the name of Metabacillus schmidteae sp. nov. (Type strain Marseille-P9898T = CSUR P9898T = DSM 111480T).

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API System: a Multitube Micromethod for Identification of Enterobacteriaceae

Abstract: The API system for identification of Enterobacteriaceae was evaluated with 366 cultures. Overall accuracy of identification was 96.4%; of the 13 cultures misidentified, 7 were atypical strains.

Cited by 155 publications

References 3 publications

Chryseobacterium schmidteae sp. nov. a novel bacterial species isolated from planarian Schmidtea mediterranea

Chryseobacterium schmidteae sp. nov. a novel bacterial species isolated from planarian Schmidtea mediterranea

Evaluation of the API 20E system for identification of nonfermentative Gram-negative bacteria

Metabacillus schmidteae sp. nov., Cultivated from Planarian Schmidtea mediterranea Microbiota

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