API System: a Multitube Micromethod for Identification of Enterobacteriaceae

Smith, P. B.; Tomfohrde, K. M.; Rhoden, D. L.; Balows, A.

doi:10.1128/aem.24.3.449-452.1972

Cited by 135 publications

(61 citation statements)

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“…Yeasts were identified by sugar fermentation (Lodder 1970), and 'germ-tube' teebniques (Mackenzie 1962). The blood agar and MacConkey's agar plates were examined for coliforms, after 18 h incubation, at 37°C and the organisms identified using the API analytical profile index (API Laboratory Products, Basingstoke, England; Smith et al 1972).…”

Section: Saliva Collection Attd Titicrobial Estimatiottmentioning

confidence: 99%

The oral carriage of yeasts and coliforms in patients on cytotoxic therapy

Samaranayake

Calman

Ferguson

et al. 1984

J Oral Pathology Medicine

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The oral carriage of yeasts and eoliforms in a healthy adult population and a group of patients with malignancies, undergoing cytotoxic therapy was investigated. A quantitative increase in the intra-oral carriage of Candida species and coliforms was observed during cytotoxic therapy. The most frequent yeast and the coliform isolated were Candida albicans and Escherichia coli, respectively. Thus, the oral Ciivity may constitute a reservoir of potentially pathogenie flora in patients on cytotoxic therapy.

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Section: Saliva Collection Attd Titicrobial Estimatiottmentioning

confidence: 99%

The oral carriage of yeasts and coliforms in patients on cytotoxic therapy

Samaranayake

Calman

Ferguson

et al. 1984

J Oral Pathology Medicine

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“…Quality control media evaluation was performed routinely at 24-48 hours and again before termination of the culture at 7-10 days. Identification o f isolates was accomplished by standard clinical microbiological methods, including the primary R/B system [2] (Diagnostic Research, Inc. Long Island, New York), supplemented as needed by standard tubed biochemicals and by API strips [3] , (Analytab Products, Plainview, New York). Antimicrobial susceptibility tests were performed by the agar overlay modification [4] of the Bauer-Kirby disc-diffusion test, accompanied by rigid control of standarization and daily processing of quality control strains.…”

Section: Microbiological Methodsmentioning

confidence: 90%

Septicemia in acute leukemia

Cesario

Slater

Armentrout

et al. 1978

Med. Pediatr. Oncol.

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We have surveyed septicemic episodes occuring in individuals with acute leukemia and have found two factors favorably influencing survival, mainly granulocyte counts over 1,000 mm3 and gram-positive bacteremias. In contrast, blood cultures persistently positive for longer than 48 hours were a bad prognosticator. Significantly, patients with gram-positive bacteremia had received less antibiotics in the week prior to septicemia than had patients with gram-negative bacteremia.

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“…Pure cultures of each organism were established by standard protocol. Isolates of gram-negative rods, Escherichia coli, Citrobacter freundii, Pasteurella pneumotropica, Pasteurella hemolytica, Pasteurella multocida, and Pseudomonas aeruginosa were speciated by biochemical and/or fermentative criteria using a standard API-20e (Analytical Profile Index) system (Analytab Products, Plainview, NY) (6). Streptococcal and staphylococcal isolates were identified by standard methods (7).…”

Section: Methodsmentioning

confidence: 99%

Non-dinitrophenyl-binding immunoglobulin that bears a dominant idiotype (Id460) associated with antidinitrophenyl antibody is specific for an antigen on Pasteurella pneumotropica.

Marion¹,

Dzierzak²,

Lee³

et al. 1984

The Journal of Experimental Medicine

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We have previously described an idiotype (Id460) that transiently dominates anti-2,4-dinitrophenyl (DNP) antibody responses of mice that possess the appropriate Igh-V and V kappa genotypes. Normal serum has significant levels of Id460 that does not bind DNP, and hybridomas derived from spleen cell fusions that produce monoclonal antibodies with these characteristics have been generated. Many of these monoclonal, Id460-positive antibodies bind the opportunistic mouse pathogen Pasteurella pneumotropica. P. pneumotropica induces a marked increase in serum Id460 titers without significantly increasing serum anti-DNP titers. Both normal serum and P. pneumotropica-induced Ig460-positive immunoglobulin specifically bind to P. pneumotropica. These results suggest that the normal serum Id460-positive immunoglobulin is induced by environmentally encountered antigens on P. pneumotropica. We propose that this naturally occurring Id460 activates antiidiotypic regulatory cells that in turn promote production of Id460-positive anti-DNP antibody following DNP-ovalbumin immunization. These data are compatible with those obtained in several other idiotypic systems that suggest that dominant idiotypes may be associated with antibodies that have been evolutionarily selected for expression because of their specificity for antigens on environmentally encountered pathogens.

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API System: a Multitube Micromethod for Identification of Enterobacteriaceae

Abstract: The API system for identification of Enterobacteriaceae was evaluated with 366 cultures. Overall accuracy of identification was 96.4%; of the 13 cultures misidentified, 7 were atypical strains.

Cited by 135 publications

References 0 publications

The oral carriage of yeasts and coliforms in patients on cytotoxic therapy

The oral carriage of yeasts and coliforms in patients on cytotoxic therapy

Septicemia in acute leukemia

Non-dinitrophenyl-binding immunoglobulin that bears a dominant idiotype (Id460) associated with antidinitrophenyl antibody is specific for an antigen on Pasteurella pneumotropica.

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