Apical periodontitis induces changes on oxidative stress parameters and increases Na+/K+-ATPase activity in adult rats

Barcelos, Raquel Cristine Silva; Rosa, Higor Zuquetto; Roversi, Karine; Tibúrcio-Machado, Camilla dos Santos; Inchaki, Paula Tassoni; Burger, Marilise Escobar; Bier, Carlos Alexandre de Souza

doi:10.1016/j.archoralbio.2020.104849

Cited by 21 publications

(18 citation statements)

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“…These results suggest the importance of deficiency of antioxidant enzymes in cardiac damage that has developed as a result of AP. Also, Barcelos et al (2020) showed that there were no changes in the CAT activity of the AP group in the heart compared to the control group, which correlates with the result of our research.…”

Section: Discussionsupporting

confidence: 92%

“…Until now there have been no studies that have analysed and compared the values of pro‐oxidants in coronary venous effluent during AP, so the results were compared with methodologically similar research. Barcelos et al (2020) showed that the experimental model of AP induced increase levels of reactive species in the rat heart, which partly coincides with the results of the study, except for the fact that the animals were hypertensive in contrast to theirs, who did not possess this systemic disease. Also, a clinical study, which dealt with connectivity endodontic infection and endothelial dysfunction, showed that values of ROS in serum were increased significantly only in women (Cotti et al, 2015).…”

Section: Discussionsupporting

confidence: 85%

“…Experimental studies have reported that AP is related to a rise in the activity of lactate dehydrogenase, creatine kinase and alkaline phosphatase, as well as reduced activity of superoxide dismutase in heart tissue (Sehirli et al, 2019). Also, AP has been reported to be associated with increased production of reactive species in the heart and decreased catalase activity and vitamin C levels in the rat heart and pancreas (Barcelos et al, 2020). On the other hand, clinical investigations suggested that patients with AP are 5.3 times more likely to have CVDs than patients without AP (An et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Cardiac, biochemical and histopathological analysis reveals impaired heart function in hypertensive rats with apical periodontitis

et al. 2021

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Aim To investigate the association between experimentally induced apical periodontitis (AP) and heart function in hypertensive rats. Methodology Forty‐eight normotensive Wistar albino and spontaneously hypertensive (SHR) rats were divided into four equal groups: control (C), normotensive with AP (AP), SHR and SHR with AP (SHR + AP). AP was induced on the first right mandibular molars by exposing the pulp chambers to the oral environment for four weeks and confirmed radiographically. Thereafter, the animals were sacrificed by cervical dislocation, whilst hearts were isolated and perfused according to the Langendorff technique gradually increasing coronary perfusion pressures 40–120 cmH2O. The hemimandibles were analysed radiographically (mm2 and pixels) to verify the presence of AP. Biomarkers of cardiac oxidative stress (OS) were determined in coronary venous effluent and cardiac tissue homogenate. Cardiac tissue was analysed histopathologically for signs of heart damage (oedema, fibrosis and necrosis). All data were analysed by Kruskal–Wallis and one‐way anova tests (p < .05). Results The levels of the maximum left ventricular pressure development rate of the SHR + AP group were significantly increased compared to the AP and C groups, and of the SHR group compared with the C group (p < .05). The levels of the minimum left ventricular pressure development rate of the SHR + AP group were significantly decreased compared to the AP, SHR and C groups, and of the SHR group compared to the C group (p < .05). The radiographic AP area was significantly larger in the SHR + AP group than in the AP group (p < .01). The levels of superoxide anion were significantly higher in the SHR + AP group than in the AP, SHR and C groups (p < .05). The activities of superoxide dismutase in cardiac tissue homogenate were significantly lower in the SHR + AP and AP groups compared with the SHR and C groups (p < .05). Conclusions In rats, AP was associated with impaired cardiodynamics, disturbed cardiac OS, antioxidant defence and cardiac pathologic changes in hypertensive conditions. Hypertension was associated with an increase in the AP radiographic area. Further studies should confirm whether root canal treatment can have a cardioprotective effect and reduce cardiac OS in hypertensive conditions.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 85%

Section: Introductionmentioning

confidence: 99%

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Cardiac, biochemical and histopathological analysis reveals impaired heart function in hypertensive rats with apical periodontitis

et al. 2021

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“…Studies in rats have been previously used in different models to assess tissue response in subcutaneous ( Bueno et al, 2016 , Bueno et al, 2019 ), apical periodontitis ( Barcelos et al, 2020 , Cosme‐Silva et al, 2020Cosme‐Silva et al, 2020Cosme‐Silva et al, 2020 ), dental alveolus ( Bueno et al, 2017 ), or periodontal ( Cintra et al, 2013 ), whether in normal or under systemic conditions, such as diabetic ( Cintra et al, 2013 , Cintra et al, 2014b ), hypertensive ( Martins et al, 2016 ) or even under alcohol consumption ( Dal‐Fabbro et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Accuracy of radiographic pixel linear analysis in detecting bone loss in periodontal disease: Study in diabetic rats

Bueno

Sumida

Duarte

et al. 2021

The Saudi Dental Journal

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Introduction Periodontitis, a complex infectious disease that may lead to irreversible loss of periodontium, is considered a predisposing agent for developing insulin resistance due to the release of inflammatory mediators, showing a bilateral relationship with diabetes mellitus. The investigation of periodontal disease requires a clinical approach and complete intraoral radiographs, even with increasing concerns about radiation exposure. Thus, this study assesses pixel linear analysis accuracy using digital radiography via Digora® in detecting alveolar bone destruction in diabetic rats with periodontal disease. Methodology 40 rats were divided into groups (n = 10): control (C), rats with periodontal disease (PD), experimental diabetic rats (ED), experimental diabetic rats with periodontal disease (ED-PD). Diabetes was induced by streptozotocin and periodontal disease by periodontal ligature. After 30 days, maxillae bone destruction was obtained by linear analysis of vertical bone loss using digital radiography and then assessed by micro-CT and histology. Data were analyzed by ANOVA and Tukey’s test (p < 0.05). Results Radiographic, micro-CT and histological analysis presented accurate and similar results. PD and ED-PD groups showed higher bone destruction than C and ED groups (p < 0.05). Moreover, the ED-PD group had higher bone loss than the PD group (p < 0.05). Conclusion The pixel linear analysis via digital radiography was an accurate, low-cost alternative in detecting alveolar bone loss in this rat model. Micro-CT and histological analysis may also be used to obtain linear measures to assess and compare periodontal bone destruction in diabetic rats.

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“…22 Elevated breakdown products of peroxidation, and reduced GSH activity in periapical granulomas are also reported. 23…”

Section: Discussionmentioning

confidence: 99%

Effect of Endodontic Treatment on Serum Glutathione (GSH) Levels in Patients with Chronic Apical Periodontitis: A Clinical Pilot Study

Bains

Tikku

Chandra

et al. 2020

Journal of Advanced Oral Research

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Aim: To assess the before- and after-treatment levels of glutathione (GSH) in the serum of patients with chronic apical periodontitis. Materials and Methods: The study involved 20 systemically healthy patients with radiographic evidence of chronic apical periodontitis in at least one tooth, of which 11 were finally assessed. 10 mL of the patient’s venous blood was obtained before commencing the endodontic treatment to assess the pre-operative serum GSH levels (T0). The canals cleaned and shaped, and irrigated with 2.5% sodium hypochlorite, and filled with a calcium hydroxide based intracanal medicament. At the subsequent appointment after one week, teeth were obturated. The patients were recalled 30 days after the completion of endodontic therapy, and blood samples were assessed again at time frame T1 for serum GSH. The difference in pre- and post-treatment values for T0 and T1 was statistically analyzed. Results: The mean age of the subjects ( N = 11) was 21.09±3.24 years, with 19.50±0.71 years in female group and 21.44±3.50 years in the male group. The mean GSH at T0 was 11.73±8.30 units, which was increased to 14.04±13.96 units at T1. However, no significant change was observed in the GSH level from T0 to T1 (change = –2.30, t-value = –0.49, P = .635). Conclusion: The results of this study suggest that endodontic therapy may play a role in restoring the systemic oxidative balance, as seen by the increase in post-treatment serum GSH levels.

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Apical periodontitis induces changes on oxidative stress parameters and increases Na+/K+-ATPase activity in adult rats

Cited by 21 publications

References 65 publications

Cardiac, biochemical and histopathological analysis reveals impaired heart function in hypertensive rats with apical periodontitis

Cardiac, biochemical and histopathological analysis reveals impaired heart function in hypertensive rats with apical periodontitis

Accuracy of radiographic pixel linear analysis in detecting bone loss in periodontal disease: Study in diabetic rats

Effect of Endodontic Treatment on Serum Glutathione (GSH) Levels in Patients with Chronic Apical Periodontitis: A Clinical Pilot Study

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