Apigenin Glucuronide from Perilla nankinensis Leaves

Elsholtzia splendens (Lamiaceae) is a copper-tolerant plant species growing on copper deposits in the south of China. Chromatographic separation of n-BuOH extracts from the flowering aerial biomass afforded apigenin-7-O-β-D-glycoside, using macroporous resin, Sephadex™ LH-20 gel, polyamide resin as well as preparative highperformance liquid chromatography (P-HPLC) columns. Chemical structure was elucidated using HPLC/ESI-MS (electrospray ionization-mass spectrometry), Fourier transform infrared (FTIR), and 1 D-and 2 D-nuclear magnetic resonance (NMR). Apigenin-7-O-β-D-glycoside could be the post-harvesting product from E. splendens biomass.

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“…1 H NMR data fit exactly with previously (Shen et al, 1993;Berashvili et al, 2005). Besides this, glycosidic δ H at 5.…”

Section: Resultssupporting

confidence: 87%

“…An FTIR spectrum (KBr) (Fig. 3) (Berashvili et al, 2005). An IR spectrum (KBr) at 3423, 1655, and 1590 cm −1 had the characteristic bands of apigenin.…”

Section: Resultsmentioning

confidence: 99%

Apigenin-7-O-β-d-glycoside isolation from the highly copper-tolerant plant Elsholtzia splendens

Peng

Zhang

2016

J. Zhejiang Univ. Sci. B

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“…Figure 6 shows the FTIR spectra of A2.I isolate. FTIR spectra of A2.I isolate shows the absence of a specific band at 1100-1000 cm -1 due to glycosidic bond [12], indicating that the A2.I isolate is not a flavonoidglycoside-furthermore, the band at 3420.42 cm -1 shows stretching vibration of the O-H group [13]. A band at 1651 cm -1 indicates the C=O stretching vibration [14].…”

Section: Structure Identification Of A2i Isolatementioning

confidence: 99%

Isolation and Antioxidant Activity of Flavonoid Compound in Ethanolic Extract of Celery Leaves (Apium graveolens L.)

2022

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Celery (Apium graveolens L.) is a plant that belongs to the Apiaceae family and is widely used as a medical plant for low blood pressure, heart tonic, and to prevent cardiovascular disease. This study aims to obtain flavonoid compounds, identify, and test the antioxidant activity of flavonoid compounds and crude extracts from celery leaves. The research procedures consisted of four steps, the first of which was a preliminary test. The second step involved isolating and separating flavonoid components by vacuum liquid chromatography, gravitational column chromatography, and preparative thin layer chromatography. The third step was to identify flavonoid compounds using reagent shift, FTIR, and LCMS/MS. And finally, antioxidant activity was evaluated using the DPPH method. The preliminary test result showed that the ethanolic extract of leaves and stems had a total flavonoid content of 13.99 and 2.46 mg QE/g of dry weight. Both dry leaves and crude extract of celery leaves contained alkaloids, saponin, flavonoid, tannin, quinone, and steroid/triterpenoid, as determined by phytochemical screening. Isolation and separation of flavonoids yielded A2.I and A2.II isolates, with respective weights of 8 mg and 14 mg. Identification of flavonoid compounds using reagent shift showed that two isolates have the basic structure of the flavone group. A2.I isolate had OH groups at 4’, 5, and 7, while A2.II isolate had OH groups at 3’, 4’, 5, and 7. The FTIR analysis revealed that both compounds contain functional groups, including O-H, C=O, C=C aromatic, C-O ether, C-O alcohol, and C-H aromatic ring. According to LCMS/MS analysis, the molecular weights of A2.I and A2.II were 270 g/mol and 286 g/mol, respectively. All of the identification methods for isolates showed that A2.I was apigenin and A2.II was luteolin. Antioxidant activity by DPPH method for a viscous extract of celery leaves, A2.I, and A2.II were 775.41, 288.95, and 184.35 µg/mL, respectively.

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SEMI-PREPARATIVE SEPARATION AND PURIFICATION OF THREE FLAVONOIDS FROM PEDICULARIS LONGIFLORA VAR. TUBIFORMIS (KLOTZSCH) P. C. TSOONG BY HSCCC

Zhao

Han

et al. 2013

Journal of Liquid Chromatography & Related Technologies

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Apigenin Glucuronide from Perilla nankinensis Leaves

Cited by 5 publications

References 7 publications

Apigenin-7-O-β-d-glycoside isolation from the highly copper-tolerant plant Elsholtzia splendens

Apigenin-7-O-β-d-glycoside isolation from the highly copper-tolerant plant Elsholtzia splendens

Isolation and Antioxidant Activity of Flavonoid Compound in Ethanolic Extract of Celery Leaves (Apium graveolens L.)

SEMI-PREPARATIVE SEPARATION AND PURIFICATION OF THREE FLAVONOIDS FROM PEDICULARIS LONGIFLORA VAR. TUBIFORMIS (KLOTZSCH) P. C. TSOONG BY HSCCC

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