APOA2 −256T>C polymorphism interacts with saturated fatty acids intake to affect anthropometric and hormonal variables in type 2 diabetic patients

Abstract

Recent studies have established the interaction between APOA2 -256T>C polymorphism and dietary saturated fatty acids intake in relation to obesity on healthy individuals. In the current study, we investigate the effects of this interaction on anthropometric variables and serum levels of leptin and ghrelin in patients with type 2 diabetes. In this cross-sectional study, 737 patients with type 2 diabetes mellitus (290 males and 447 females) were recruited from diabetes clinics in Tehran. The usual dietary intake of all participants during the last year was obtained by validated semiquantitative food frequency questionnaire. APOA2 genotyping was performed by real-time PCR on genomic DNA. No significant relation was obtained by univariate analysis between anthropometric variables and APOA2 genotypes. However, after adjusting for age, gender, physical activity and total energy intake, we identified a significant interaction between APOA2-saturated fatty acids intake and body mass index (BMI). After adjusting for potential confounders, serum levels of ghrelin in CC genotype patients were significantly higher than T allele carriers (p = 0.03), whereas the case with leptin did not reveal a significant difference. The result of this study confirmed the interaction between APOA2 -256T>C polymorphism and SFAs intake with BMI in type 2 diabetic patients. In fact, homozygous patients for the C allele with high saturated fatty acids intake had higher BMI. The APOA2 -256T>C polymorphism was associated with elevated levels of serum ghrelin.

APO A2 -265T/C Polymorphism Is Associated with Increased Inflammatory Responses in Patients with Type 2 Diabetes Mellitus

“…Sample size was defined according to a type I error of α=0.05 and a type II error of β=80%. In total, 180 T2DM patients, divided into two groups of 90 individuals with or without obesity (BMI ≥30 or <30 kg/m 2 ), were selected from 816 previously genotyped individuals (the frequency of the APO A2 genotypes was 39.4%, 47.7%, and 12.9% for the TT, TC, and CC genotypes combinations, respectively) [ 18 ]. In the current study, each group contained 30 patients for each TT, TC, or CC genotypes.…”

Association between ApoA-II -265T/C polymorphism and oxidative stress in patients with type 2 diabetes mellitus

“…Of 816 patients with type 2 diabetes who were examined in an earlier study for ApoA-II genotypes (Basiri et al, 2015), two obese (BMI ≥ 30) and non-obese (BMI b 30) groups were selected. Each group included 90 patients with equal numbers of each genotype of TT, CC and TC.…”

Dietary Fatty Acid Composition Modulates Obesity and Interacts with Obesity‐Related Genes

“…[179] 2280 non-Hispanic whites men and women/survey APOA5 t /rs662799 and rs3135506 Rs662799 C minor allele carriers with the high MUFA intake had a lower obesity risk compared with TT carriers [180] 3462 Whites and Hispanic men and women/follow-up survey APOA2 u /rs5082 CC genotype with a high SFA intake was associated with a higher obesity prevalence [181] 2071 Puerto Ricans origin and Northern European ancestry men and women/survey APOA2 u /rs5082 CC carriers who consumed a greater amount of SFA from a high-fat dairy foods had a greater BMI than those who consume less dairy fat [182] 737 Iranian diabetic patients men and women/survey APOA2 u /rs5082 Carriers of CC allele with a high SFA intake had higher BMI [165] 200 Mexican normal-weight and obese men and women/ survey APOA2 u /rs3813627 and rs5082 No interaction was found between SFA intake and APOA2 SNP [183] 1225 Spanish overweight and obese men and women/ survey APOA2 u /rs5082 A high SFA intake was associated with a greater WC in minor allele homozygotes CC compared with non-minor allele carriers [184] 4602 Asian and Mediterranean obese men and women/ survey…”